C. Keith Ozaki, MD, Matthew D. Phaneuf, BS, Suchen L

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Presentation transcript:

Glycoconjugate mediated endothelial cell adhesion to Dacron polyester film* C.Keith Ozaki, MD, Matthew D. Phaneuf, BS, Suchen L. Hong, PhD, William C. Quist, MD, PhD, Frank W. LoGerfo, MD Journal of Vascular Surgery Volume 18, Issue 3, Pages 486-494 (September 1993) DOI: 10.1016/0741-5214(93)90267-P Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 EDC-mediated conjugation of UEA I to PET. Journal of Vascular Surgery 1993 18, 486-494DOI: (10.1016/0741-5214(93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Schematic demonstrates biotinylated neoglycoprotein fucosylamide albumin binding to immobilized lectin. This interaction is documented with fluorescent avidin. Journal of Vascular Surgery 1993 18, 486-494DOI: (10.1016/0741-5214(93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Binding of Na[125I]-labeled UEA I to hydrolyzed PET with cross-linker EDC. Covalent = (Covalent + Nonspecific) − nonspecific. Journal of Vascular Surgery 1993 18, 486-494DOI: (10.1016/0741-5214(93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 IAEC adherence to various PET surfaces (n = 4 independent experiments for each). Significantly more cells bound to the UEA I/PET surface than to any control surface (Tukey-Kramer multiple comparisons). Journal of Vascular Surgery 1993 18, 486-494DOI: (10.1016/0741-5214(93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Each of five vascular endothelial cell lines tested adhered to UEA I/PET surface, whereas monocytes, SMCs, and fibroblasts did not (n = 4 independent experiments for each). Journal of Vascular Surgery 1993 18, 486-494DOI: (10.1016/0741-5214(93)90267-P) Copyright © 1993 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions