Volume 3, Issue 1, Pages 192-211 (January 2010) Metabolomic, Transcriptional, Hormonal, and Signaling Cross-Talk in Superroot2  Morant Marc , Ekstrøm Claus , Ulvskov Peter , Kristensen Charlotte , Rudemo Mats , Olsen Carl Erik , Hansen Jørgen , Jørgensen Kirsten , Jørgensen Bodil , Møller Birger Lindberg , Bak Søren   Molecular Plant  Volume 3, Issue 1, Pages 192-211 (January 2010) DOI: 10.1093/mp/ssp098 Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 1 Phenotype of 10-Day-Old Seedlings Grown on Vertical Agar Plates Showing Phenotypic Differences between sur2 Knockout Mutant and Wild-Type Seedlings. (A) The white arrow underlines the heterogeneity observed among the sur2 mutants. (B) Transverse section of plastic embedded hypocotyls of wild-type and sur2 showing radial expansion of cortical cells and close-up of adventitious root on the hypocotyls. Molecular Plant 2010 3, 192-211DOI: (10.1093/mp/ssp098) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 2 Schematic Representation of Indole Glucosinolate Pathway Showing the Importance of Indole-3-Acetaldoxime as a Central Metabolite for Indole Secondary Metabolism in Arabidopsis thaliana. R can either be a glucose moiety or an amino acid. Known positive regulators of transcription involved in this grid are indicated in magenta. Molecular Plant 2010 3, 192-211DOI: (10.1093/mp/ssp098) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 3 Biological Processes Affected in the sur2 Knockout Mutant Based on Expression Analysis using the Agilent 22K Array. The 301 genes most significantly affected in their expression were analyzed in BiNGO in order to identify the biological processes significantly affected. The figure is based on the data extracted from the BiNGO analysis (Supplemental Data 2). The representation includes sorting by p-value (color) and size of each group. When GO-groups only referred to a small number of genes, those genes were preferred. Molecular Plant 2010 3, 192-211DOI: (10.1093/mp/ssp098) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 4 Metabolites Identified as Accumulating at Significantly Different Levels in the sur2 Mutant and Wild-Type Plants Based on Average Values. Columns represent the metabolites that were significantly detected as up- or down-regulated with a cut-off of two-fold and with a p-value of 0.0001 (see Supplemental File 2). Major metabolites subsequently identified are represented with a *. Sinapoyl glucose (M10) had less than a two-fold variation, but was kept because of its high intensity and because it is known to be co-regulated with sinapoyl malate (M3). Molecular Plant 2010 3, 192-211DOI: (10.1093/mp/ssp098) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 5 Chemical Structures of the Metabolites Identified as Accumulating at Different Levels in the sur2 Mutant and Wild-Type Plants. Molecular Plant 2010 3, 192-211DOI: (10.1093/mp/ssp098) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

Figure 6 Diagram Displaying the Major Intrinsic Metabolomic Network in sur2 Mutant. Molecular Plant 2010 3, 192-211DOI: (10.1093/mp/ssp098) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions