Chromatin Starts to Come Clean

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Chromatin Starts to Come Clean Shengjiang Tu, Gary LeRoy, Danny Reinberg Molecular Cell Volume 64, Issue 3, Pages 439-441 (November 2016) DOI: 10.1016/j.molcel.2016.10.022 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 Schematic Diagram of ChIP-SICAP (A) Formaldehyde cross-linked sheared chromatin prepared from cells. (B) Immunoprecipitation of nucleosomal chains and associated proteins with antibody specific to target protein. (C) Wash immunoprecipitate followed by addition of Biotin-ddUTP by TdT. (D) Precipitate with Strepavidin beads. (E) Successive stringent washes followed by elution and reversal of cross-links. (F) Proteins are trypsinized and analyzed by quantitative mass spectrometry. Molecular Cell 2016 64, 439-441DOI: (10.1016/j.molcel.2016.10.022) Copyright © 2016 Elsevier Inc. Terms and Conditions