Perigraft vascularization and incorporation of implanted Dacron prostheses are affected by rifampicin coating Mohammed R. Moussavian, MD, Matthias W.

Slides:

Advertisements

Similar presentations

Tumor-Induced Sentinel Lymph Node Lymphangiogenesis and Increased Lymph Flow Precede Melanoma Metastasis Maria I. Harrell, Brian M. Iritani, Alanna Ruddell

Advertisements

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Diet-induced obesity drives negative mouse vein graft wall remodeling

Selective cyclo-oxygenase-2 inhibition induces regression of autologous endometrial grafts by down-regulation of vascular endothelial growth factor-mediated.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff Akio Koyama, MD, Kimihiro.

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD

Robert J. Pitsch, MD, David J. Minion, MD, Margaret L

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis April E. Nedeau, MD,

The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.

Effect of tissue plasminogen activator on vascular smooth muscle cells

Integrin α2β1 Is Required for Regulation of Murine Wound Angiogenesis but Is Dispensable for Reepithelialization Manon C. Zweers, Jeffrey M. Davidson,

Angiogenic effects of stromal cell-derived factor-1 (SDF-1/CXCL12) variants in vitro and the in vivo expressions of CXCL12 variants and CXCR4 in human.

Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft Hongshan Zhong, MD, Osamu Matsui, MD,

VEGF Gene Delivery to Muscle

Infection of vascular prostheses caused by bacterial biofilms

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Gene transduction into aortic wall using plasmid-loaded cationized gelatin hydrogel- coated polyester stent graft Hongshan Zhong, MD, Osamu Matsui, MD,

Matthias W. Laschke, M. D. , Antje Elitzsch, D. V. M

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff Akio Koyama, MD, Kimihiro.

Jeanwan Kang, MD, Hassan Albadawi, MD, Virendra I. Patel, MD, Thomas A

Robert A. Brenes, MD, Caroline C

Blood-derived smooth muscle cells as a target for gene delivery

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold

Increased vascularization of shoulder regions of carotid atherosclerotic plaques from patients with diabetes Fredrik J. Olson, PhD, Sofia Strömberg,

Prevention of perioperative vascular prosthetic infection with a novel triple antimicrobial- bonded arterial graft Ibrahim Aboshady, MD, Issam Raad, MD,

Silver acetate coating promotes early vascularization of Dacron vascular grafts without inducing host tissue inflammation Pascal Jeanmonod, MD, Matthias.

Ulka Sachdev, MD, Xiangdong Cui, MS, Edith Tzeng, MD

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Cellular and molecular mechanism regulating blood flow recovery in acute versus gradual femoral artery occlusion are distinct in the mouse Yagai Yang,

Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material Soichiro Enomoto, MD, PhD, Makoto Sumi, MD,

Murine abdominal aortic aneurysm model by orthotopic allograft transplantation of elastase-treated abdominal aorta Zhenjie Liu, MD, PhD, Qiwei Wang,

Nanoparticle-mediated endothelial cell-selective delivery of pitavastatin induces functional collateral arteries (therapeutic arteriogenesis) in a rabbit.

Diet-induced obesity drives negative mouse vein graft wall remodeling

Matthias W Laschke, Michael D Menger, M.D., Brigitte Vollmar, M.D.

A pilot study of a triple antimicrobial-bonded Dacron graft for the prevention of aortic graft infection Ibrahim Aboshady, MD, Issam Raad, MD, Aamir.

Proof of fallout endothelialization of impervious Dacron grafts in the aorta and inferior vena cava of the dog Qun Shi, MD, Moses Hong-De Wu, MD, Naoki.

Mechanisms of arterial graft failure. II

Spatiotemporal expression and localization of matrix metalloproteinas-9 in a murine model of thoracic aortic aneurysm Jeffrey A. Jones, PhD, John R.

Leptin receptor is elevated in carotid plaques from neurologically symptomatic patients and positively correlated with augmented macrophage density Jacob.

Jing Wen, MD, Peiyi Wang, MS, Sumona V. Smith, MD, Carolyn A

Dietary glycine does not affect physiological angiogenesis and reproductive function, but inhibits apoptosis in endometrial and ovarian tissue by down-regulation.

Matthias W. Laschke, M. D. , Antje Elitzsch, D. V. M

In vivo suppression of vein graft disease by nonviral, electroporation-mediated, gene transfer of tissue inhibitor of metalloproteinase-1 linked to the.

Surgical marking pen dye inhibits saphenous vein cell proliferation and migration in saphenous vein graft tissue Shinsuke Kikuchi, MD, Richard D. Kenagy,

The coronary delivery of marrow stromal cells for myocardial regeneration: Pathophysiologic and therapeutic implications Jih-Shiuan Wang, MD, Dominique.

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Seung-Kee Min, MD, PhD, Richard D. Kenagy, PhD, Joseph P

Delayed inhaled carbon monoxide mediates the regression of established neointimal lesions Michael Madigan, MD, Fateh Entabi, MD, Brian Zuckerbraun, MD,

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

New assessment of platelet deposition in small caliber vascular prostheses using technetium-99m apcitide scintigraphy in rabbit model Tomomi Hasegawa,

Increased Microvascular Density and Enhanced Leukocyte Rolling and Adhesion in the Skin of VEGF Transgenic Mice Michael Detmar, Lawrence F. Brown, Michael.

Shadi J. Abu-Halimah Journal of Vascular Surgery

Subacute limb ischemia induces skeletal muscle injury in genetically susceptible mice independent of vascular density Joseph M. McClung, PhD, Timothy.

Michael A. Rosenbaum, MD, Keiko Miyazaki, MD, Linda M. Graham, MD

Lisheng Zhang, MD, Leigh Brian, MS, Neil J. Freedman, MD

Neointimal hyperplasia on a cell-seeded polytetrafluoroethylene graft is promoted by transfer of tissue plasminogen activator gene and inhibited by transfer.

Autologous skeletal myoblasts transduced with a new adenoviral bicistronic vector for treatment of hind limb ischemia Muhammad I. Niagara, MSc, Husnain.

Novel Anti-Inflammatory Properties of the Angiogenesis Inhibitor Vasostatin Rainer Huegel, Paula Velasco, Maria De La Luz Sierra, Enno Christophers, Jens.

Development of a spontaneously beating vein by cardiomyocyte transplantation in the wall of the inferior vena cava in a rat: A pilot study Wangde Dai,

Reviewer Acknowledgment

Blockade of monocyte chemoattractant protein-1 by adenoviral gene transfer inhibits experimental vein graft neointimal formation Hideki Tatewaki, MD,

Prevention of perioperative vascular prosthetic infection with a novel triple antimicrobial- bonded arterial graft Ibrahim Aboshady, MD, Issam Raad, MD,

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Adenovirus-mediated gene transfer of human inducible nitric oxide synthase in porcine vein grafts inhibits intimal hyperplasia Melina R. Kibbe, MDa,

George D. Lilly 1906–1988 Journal of Vascular Surgery

Marrow stromal cells for cellular cardiomyoplasty: Feasibility and potential clinical advantages Jih-Shiuan Wang, MDa,b, Dominique Shum-Tim, MDa, Jacques.

Presentation transcript:

Perigraft vascularization and incorporation of implanted Dacron prostheses are affected by rifampicin coating Mohammed R. Moussavian, MD, Matthias W. Laschke, MD, Georg Schlachtenberger, Maximilian von Heesen, MD, Matthias Wagner, MD, Matthias Glanemann, MD, Michael D. Menger, MD Journal of Vascular Surgery Volume 64, Issue 6, Pages 1815-1824 (December 2016) DOI: 10.1016/j.jvs.2015.07.104 Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 1 Intravital fluorescent microscopic images of the border of (A) a Dacron-Con (asterisk) and (B) a Dacron-Rifamp graft (asterisk) at day (d) 14 after implantation into the dorsal skinfold chamber of C57BL/6 mice. Note the high density of newly formed microvessels at the border of (A) Dacron-Con (arrows). B, Dacron-Rifamp shows only a few newly formed microvessels (arrows). Blue light epi-illumination with contrast enhancement by 5% fluorescein isothiocyanate-labeled intravenous dextran 150,000. C, Functional capillary density (cm/cm2) within the border of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8), assessed using intravital fluorescence microscopy and computer-assisted image analysis. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 500 μm. Journal of Vascular Surgery 2016 64, 1815-1824DOI: (10.1016/j.jvs.2015.07.104) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 2 Immunohistochemical cross sections of the border zones of (A) a Dacron-Con and (B) a Dacron-Rifamp graft at day 14 after implantation in the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with Hoechst 33,342 to identify cell nuclei (blue) and with an antibody against CD31 for the visualization of microvessels (red). Note the higher microvessel density in the border zone of the (A) Dacron-Con compared with the (B) Dacron-Rifamp graft. C, Microvessel density (mm2) in the border zones of Dacron-Con (white bar; n = 8) and Dacron-Rifamp grafts (black bar; n = 8) at day 14 after implantation. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 125 μm. Journal of Vascular Surgery 2016 64, 1815-1824DOI: (10.1016/j.jvs.2015.07.104) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 3 A, Rolling leukocytes (per minute) and (B) adherent leukocytes (mm2) in postcapillary and collecting venules within the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8), assessed using intravital fluorescence microscopy and computer-assisted image analysis. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. d, Day. Journal of Vascular Surgery 2016 64, 1815-1824DOI: (10.1016/j.jvs.2015.07.104) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 4 Immunohistochemical cross sections of the border zones of (A-C) a Dacron-Con and (D-F) a Dacron-Rifamp graft at day 14 after implantation into the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with an antibody against myeloperoxidase (MPO) for the detection of neutrophils (A and D, arrows), an antibody against CD3 for the detection of lymphocytes (B and E, arrow), and an antibody against MAC387 for the detection of macrophages (C and F, arrows). G-I, MPO-positive neutrophils (mm2), CD3-positive lymphocytes (mm2), and MAC387-positive macrophages (mm2) in the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n =8) at day 14 after implantation. Mean ± standard error of the mean. A-F, Scale bar = 125 μm. Journal of Vascular Surgery 2016 64, 1815-1824DOI: (10.1016/j.jvs.2015.07.104) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 5 Immunohistochemical cross sections of the border zones of (A) a Dacron-Con and (B) a Dacron-Rifamp graft at day 14 after implantation into the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with an antibody against Ki-67 for the visualization of proliferating cells (arrows). C, Ki-67-positive cells (%) in the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8) at day 14 after implantation. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 125 μm. Journal of Vascular Surgery 2016 64, 1815-1824DOI: (10.1016/j.jvs.2015.07.104) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 6 Immunohistochemical cross sections of the border zones of (A) a Dacron-Con and (B) a Dacron-Rifamp graft at day 14 after implantation into the striated muscle tissue within the dorsal skinfold chamber of C57BL/6 mice. The sections were stained with an antibody against caspase-3 (casp-3) for the visualization of apoptotic cells (arrows). C, casp-3-positive cells (%) in the border zones of Dacron-Con (white bars; n = 8) and Dacron-Rifamp grafts (black bars; n = 8) at day 14 after implantation. Mean ± standard error of the mean. ∗P < .05 vs Dacron-Con. A and B, Scale bar = 125 μm. Journal of Vascular Surgery 2016 64, 1815-1824DOI: (10.1016/j.jvs.2015.07.104) Copyright © 2015 Society for Vascular Surgery Terms and Conditions