Grading of smears examined in ordinary microscopy at 1000X magnification after ZN staining Exercise: Quantification of AFB in ZN Smears.

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Presentation transcript:

Grading of smears examined in ordinary microscopy at 1000X magnification after ZN staining Exercise: Quantification of AFB in ZN Smears

----- smear contains no AFB in 100 fields. NEG

How will you grade a smear with 9 AFB in 100 fields ? positive(Actual) 09 AFB

------ Smear with more than 10 AFB per field. 3 +

A smear with 1 to 9 bacilli in majority of the fields will be graded as: 2 +

(1-9 AFB /field in at least 50 fields) Smear grade: 2 + (1-9 AFB /field in at least 50 fields) Exercise: Quantification of AFB in ZN Smears

10 - 99 AFB in 100 fields Smear Grade: 1 + Exercise: Quantification of AFB in ZN Smears

Smear Grade:actual ( < 10 AFB in 100 fields) Exercise: Quantification of AFB in ZN Smears

Grade the following smears: Exercise: Quantification of AFB in ZN Smears

Exercise: Quantification of AFB in ZN Smears

Positive (6AFB) Exercise: Quantification of AFB in ZN Smears

Exercise: Quantification of AFB in ZN Smears

Positive 1+ Exercise: Quantification of AFB in ZN Smears

Exercise: Quantification of AFB in ZN Smears

1 + (86 AFB /96 HPF) Exercise: Quantification of AFB in ZN Smears

Exercise: Quantification of AFB in ZN Smears

08 AFB (Actual)

Exercise: Quantification of AFB in ZN Smears

Positive 2 + Exercise: Quantification of AFB in ZN Smears

Exercise: Quantification of AFB in ZN Smears

Positive (8 AFB) Exercise: Quantification of AFB in ZN Smears

Exercise: Quantification of AFB in ZN Smears

2 +

Exercise: Quantification of AFB in ZN Smears

Positive 1 + Exercise: Quantification of AFB in ZN Smears

Credit Revised National Tuberculosis Control Program-Tuberculosis Research Center Chennai, India

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