Introduction Glycolysis Bacterial Catabolism Introduction Glycolysis

Bacterial Catabolism Introduction Catabolic activities include the break down of glucose to release energy in form of ATP Reactions include three pathways Glycolysis Krebs’ Cycle Oxidative Phosphorylation (Electron Transport) Alternative pathways

Bacterial Catabolism Glycolysis - Includes: Activation Oxidation ATP Synthesis NADH2 Production

Bacterial Catabolism Glycolysis “splitting” of sugar Glucose to pyruvate Substrate phosphorylation 10 reactions / two phases Activation Energy Production Evolution - probably oldest

Bacterial Catabolism

Bacterial Catabolism Activation of glucose by ATP

Bacterial Catabolism Activate with second ATP

Bacterial Catabolism Split sugar into 2 x 3C molecules

Bacterial Catabolism Oxidation and phosphate addition

Bacterial Catabolism ATP made at substrate level from 1,3-PGA

Bacterial Catabolism

Bacterial Catabolism

Bacterial Catabolism Second ATP made from PEP

Bacterial Catabolism Summary of glycolysis 1 Glucose to 2 pyruvate 2 NADH2 produced 2 ATP consumed 2 ATP for each 3C molecule produced Net yield of 2 ATP

Bacterial Catabolism Anaerobic Fermentation NADH2 must be recycled for glycolysis to continue to produce ATP Pyruvate to lactate Pyruvate to ethanol & CO2 Pyruvate to propionate

Bacterial Catabolism

Bacterial Catabolism