Ang II induces translocation of α-ENaC toward the apical membrane. Ang II induces translocation of α-ENaC toward the apical membrane. A, representative confocal plane images (top) and corresponding cross-sections of three-dimensional stacks by z axis (bottom) of α-ENaC localization (pseudocolor green) in split-opened ASDNs treated for 30 min with vehicle (left), 500 nm Ang II (middle), and 10 μm forskolin (right). Nuclear DAPI staining is also shown (pseudocolor blue). The positions of cross-section are shown by the arrows. B, distribution of averaged relative fluorescent signals representing α-ENaC localization along a line on z axis crossing a cell upon treatment with vehicle, Ang II, and forskolin. For each individual cell the fluorescent signals were normalized to their corresponding maximal value. The positions of the apical and basolateral sides are shown with arrows at the top. C, summary graph of half-width means for distributions of fluorescent signals shown in B. *, significant decrease versus control. Error bars, S.E. Mykola Mamenko et al. J. Biol. Chem. 2012;287:660-671 © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.