N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress Monireh Mahmoodi, Malek Soleimani.

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N-acetylcysteine improves function and follicular survival in mice ovarian grafts through inhibition of oxidative stress Monireh Mahmoodi, Malek Soleimani Mehranjani, Seyed Mohammad Ali Shariatzadeh, Hussein Eimani, Abdulhussein Shahverdi Reproductive BioMedicine Online Volume 30, Issue 1, Pages 101-110 (January 2015) DOI: 10.1016/j.rbmo.2014.09.013 Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Microscopic images of mice ovary tissues in different groups 28 days after autotransplantation. (A) The control group: follicles are observed in different development stages; (B) autograft plus saline group: fewer follicles are observed in different development stages; (C, c) autograft plus N-acetylcysteine group: a considerable number of different primordial, growing, antral and preovulatory follicles are observed compared with B. Arrows showing the different types of follicles; and (D) another cross-section of the ovaries in the autograft plus N-acetylcysteine group demonstrating the corpus luteum (*). Scale bar = 100 µm. Reproductive BioMedicine Online 2015 30, 101-110DOI: (10.1016/j.rbmo.2014.09.013) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Histomorphological evaluation of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) staining in ovarian grafts 28 days after transplantation. (A) Control group: little sign of apoptosis was detected; (B) saline-treated group; (C) N-acetylcysteine-treated group: few apoptotic cells were observed in the stroma and follicles. Arrows indicate TUNEL-positive follicular cells (dark brown nucleus). Scale bar = 100 µm. Reproductive BioMedicine Online 2015 30, 101-110DOI: (10.1016/j.rbmo.2014.09.013) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Comparison of apoptosis rate in different groups of mice 28 days after heterotopic ovarian autotransplantation and treatment with N-acetylcysteine (150 mg/kg intraperitoneal injection). Values presented as mean ± SD and the different code letters are considered significantly different (one way analysis of variance and Tukey's test); a versus b P < 0.001. NAC = N-acetylcysteine. Reproductive BioMedicine Online 2015 30, 101-110DOI: (10.1016/j.rbmo.2014.09.013) Copyright © 2014 Reproductive Healthcare Ltd. Terms and Conditions