IL-5 receptor α levels in patients with marked eosinophilia or mastocytosis Todd M. Wilson, DO, Irina Maric, MD, Juhi Shukla, MSc, Margaret Brown, BS,

Slides:

Advertisements

Similar presentations

Elevated serum tryptase levels identify a subset of patients with a myeloproliferative variant of idiopathic hypereosinophilic syndrome associated with.

Advertisements

High-titer IgE antibody specific for pollen allergens in northern California is associated with both wheezing and total serum IgE Elizabeth A. Erwin,

Production of IL-5 and granulocyte-macrophage colony-stimulating factor by naive human mast cells activated by high-affinity IgE receptor ligation Robert.

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection

Nerve growth factor is preformed in and activates human peripheral blood eosinophils Abraham Solomon, MDa,b, Luigi Aloe, PhDc, Jacob Pe’er, MDa, Joseph.

Toll-like receptor 7–induced naive human B-cell differentiation and immunoglobulin production Mark C. Glaum, MD, PhD, Shilpi Narula, MD, Decheng Song,

Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,

Siglec-7 is an inhibitory receptor on human mast cells and basophils

Julie M. Caldwell, PhD, Carine Blanchard, PhD, Margaret H

Anti–IL-5 (mepolizumab) therapy reduces eosinophil activation ex vivo and increases IL- 5 and IL-5 receptor levels Miguel L. Stein, MD, Joyce M. Villanueva,

Fanny Legrand, PhD, PharmD, Amy D. Klion, MD

Defective killing of Staphylococcus aureus in atopic dermatitis is associated with reduced mobilization of human β-defensin-3 Kevin O. Kisich, PhD, Charles.

MEDI-563, a humanized anti–IL-5 receptor α mAb with enhanced antibody-dependent cell-mediated cytotoxicity function Roland Kolbeck, PhD, Alexander Kozhich,

CXCR4/IgG-expressing plasma cells are associated with human gastrointestinal tissue inflammation Clarisa M. Buckner, PhD, Susan Moir, PhD, Lela Kardava,

D prostanoid receptor 2 (chemoattractant receptor–homologous molecule expressed on TH2 cells) protein expression in asthmatic patients and its effects.

Efficient cytokine-induced IL-13 production by mast cells requires both IL-33 and IL-3 Ilkka S. Junttila, MD, PhD, Cynthia Watson, BSc, Laura Kummola,

Human mast cells arise from a common circulating progenitor

Dawn C. Newcomb, PhD, Madison G

Serum periostin is associated with type 2 immunity in severe asthma

Silvana Balzar, MD, Matthew Strand, PhD, Diane Rhodes, PhD, Sally E

Serum IL-31 levels are increased in a subset of patients with mastocytosis and correlate with disease severity in adult patients Karin Hartmann, MD,

The innate antiviral response upregulates IL-13 receptor α2 in bronchial fibroblasts Gemma Campbell-Harding, PhD, Hannah Sawkins, BSc, Nicole Bedke, PhD,

Frank Kirstein, PhD, Natalie E

Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps Sarah Peterson, MD, Julie A. Poposki, MS, Deepti.

Mastocytosis associated with a rare germline KIT K509I mutation displays a well- differentiated mast cell phenotype Eunice Ching Chan, PhD, Yun Bai, MSc,

Eosinophilia in systemic mastocytosis: Clinical and molecular correlates and prognostic significance Alexandra Böhm, MD, Manuela Födinger, MD, Friedrich.

KIT D816V–associated systemic mastocytosis with eosinophilia and FIP1L1/PDGFRA- associated chronic eosinophilic leukemia are distinct entities Irina Maric,

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

T-cell receptor diversity is selectively skewed in T-cell populations of patients with Wiskott-Aldrich syndrome Junfeng Wu, MD, Dawei Liu, MS, Wenwei.

Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps Dae Woo Kim, MD, PhD, Marianna.

The usefulness of measuring tear periostin for the diagnosis and management of ocular allergic diseases Hiroshi Fujishima, MD, Naoko Okada, PhD, Kenji.

David M. Pyle, BS, Victoria S. Yang, MD, Rebecca S

Human mast cells drive memory CD4+ T cells toward an inflammatory IL-22+ phenotype Nicolas Gaudenzio, PhD, Camille Laurent, MD, Salvatore Valitutti,

Carine Blanchard, PhD, Emily M

Human mast cells are major IL-22 producers in patients with psoriasis and atopic dermatitis Shunya Mashiko, BPharm, Salim Bouguermouh, MD, PhD, Manuel.

“Auto-anti-IgE”: Naturally occurring IgG anti-IgE antibodies may inhibit allergen-induced basophil activation Yih-Chih Chan, PhD, Faruk Ramadani, PhD,

Allison K. Martin, BS, Douglas G. Mack, PhD, Michael T

Mast cell activation syndrome: Proposed diagnostic criteria

Jon Genuneit, MD, MSc Journal of Allergy and Clinical Immunology

Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis Deepti R. Nagarkar, PhD, Julie A. Poposki,

A distinct biomolecular profile identifies monoclonal mast cell disorders in patients with idiopathic anaphylaxis Melody C. Carter, MD, Avanti Desai,

Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,

Expression of programmed cell death ligand-1 in mastocytosis correlates with disease severity Anja Rabenhorst, PhD, Silke Leja, MTA, Juliana Schwaab,

Statins enhance the anti-inflammatory effects of inhaled corticosteroids in asthmatic patients through increased induction of indoleamine 2, 3-dioxygenase

CC chemokine receptors CCR1 and CCR4 are expressed on airway mast cells in allergic asthma Kawa Amin, PhD, Christer Janson, MD, PhD, Ilkka Harvima, MD,

Assessment of clinical findings, tryptase levels, and bone marrow histopathology in the management of pediatric mastocytosis Melody C. Carter, MD, Sarah.

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

Mepolizumab as a corticosteroid-sparing agent in lymphocytic variant hypereosinophilic syndrome Florence Roufosse, MD, PhD, Aurore de Lavareille, PhD,

Changes in regulatory B-cell levels in bone marrow, blood, and sputum of patients with asthma following inhaled allergen challenge John-Paul Oliveria,

Aspirin-sensitive rhinosinusitis is associated with reduced E-prostanoid 2 receptor expression on nasal mucosal inflammatory cells Sun Ying, MD, PhD,

Huie Jing, PhD, Qian Zhang, MD, Yu Zhang, PhD, Brenna J

Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,

Analysis of the lineage relationship between mast cells and basophils using the c-kit D816V mutation as a biologic signature Can N. Kocabas, MD, Akif.

Rebound eosinophilia after treatment of hypereosinophilic syndrome and eosinophilic gastroenteritis with monoclonal anti–IL-5 antibody SCH55700 Yae-Jean.

The eosinophil surface receptor epidermal growth factor–like module containing mucin- like hormone receptor 1 (EMR1): A novel therapeutic target for eosinophilic.

Expression of chemokines and chemokine receptors in lesional and nonlesional upper skin of patients with atopic dermatitis Eva Gros, MSc, Caroline Bussmann,

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

IL-4 upregulates FcϵRI α-chain messenger RNA in eosinophils

A flow cytometry–based diagnosis of eosinophilic esophagitis

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

Peripheral blood and airway tissue expression of transforming growth factor β by neutrophils in asthmatic subjects and normal control subjects Hong Wei.

Cigarette smoke exposure is associated with vitamin D3 deficiencies in patients with chronic rhinosinusitis Jennifer K. Mulligan, PhD, Whitney Nagel,

The eosinophil surface receptor epidermal growth factor–like module containing mucin- like hormone receptor 1 (EMR1): A novel therapeutic target for eosinophilic.

Karl J. Staples, PhD, Timothy S. C. Hinks, MB BS, Jon A

Statins enhance the anti-inflammatory effects of inhaled corticosteroids in asthmatic patients through increased induction of indoleamine 2, 3-dioxygenase

Transforming growth factor β1 increases fibronectin deposition through integrin receptor α5β1 on human airway smooth muscle Lyn M. Moir, PhD, Janette.

Carine Blanchard, PhD, Emily M

Alexander S. Kim, MD, Taylor A. Doherty, MD, Maya R

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Advances in mechanisms of allergic disease in 2016

Presentation transcript:

IL-5 receptor α levels in patients with marked eosinophilia or mastocytosis Todd M. Wilson, DO, Irina Maric, MD, Juhi Shukla, MSc, Margaret Brown, BS, Carlo Santos, MSc, Olga Simakova, PhD, Paneez Khoury, MD, Michael P. Fay, PhD, Alexander Kozhich, PhD, Roland Kolbeck, PhD, Dean D. Metcalfe, MD, Amy D. Klion, MD Journal of Allergy and Clinical Immunology Volume 128, Issue 5, Pages 1086-1092.e3 (November 2011) DOI: 10.1016/j.jaci.2011.05.032 Copyright © 2011 Terms and Conditions

Fig 1 Serum levels of sIL-5Rα are positively correlated with peripheral blood EO. Each circle represents an individual data point. Subjects with eosinophilic disease are shown in red, those with mastocytosis in blue, and normals in green. The dotted line represents the limit of detection of the assay. r = 0.54; P < .0001; Spearman rank correlation. Journal of Allergy and Clinical Immunology 2011 128, 1086-1092.e3DOI: (10.1016/j.jaci.2011.05.032) Copyright © 2011 Terms and Conditions

Fig 2 Serum IL-5Rα levels are increased in patients with EO and mastocytosis without EO. Each symbol represents an individual data point. Subjects with EO are indicated in red, with mastocytosis in blue, and normal controls in green. Group GMs are indicated by horizontal lines. ∗P < .001 or †P < .05 compared with normal group (Holm’s adjusted P value using the Mann-Whitney U test). Journal of Allergy and Clinical Immunology 2011 128, 1086-1092.e3DOI: (10.1016/j.jaci.2011.05.032) Copyright © 2011 Terms and Conditions

Fig 3 Surface expression of IL-5Rα on eosinophils is inversely correlated with peripheral blood EO. Each circle represents an individual data point. Subjects with eosinophilic disease are shown in red, those with mastocytosis in blue, and normal controls in green. ABC, Antibodies bound per cell. r = −0.48; P < .0001; Spearman rank correlation. Journal of Allergy and Clinical Immunology 2011 128, 1086-1092.e3DOI: (10.1016/j.jaci.2011.05.032) Copyright © 2011 Terms and Conditions

Fig 4 Serum levels of sIL-5Rα are correlated with serum tryptase levels in patients with SM, but not HES without SM. Serum tryptase and sIL-5Rα levels are shown for subjects with SM (r = 0.62; P < .0001; Spearman rank correlation, panel A) and EO without SM (r = 0.19; P = .2; panel B). Each circle represents an individual data point. Journal of Allergy and Clinical Immunology 2011 128, 1086-1092.e3DOI: (10.1016/j.jaci.2011.05.032) Copyright © 2011 Terms and Conditions

Fig E1 Bone marrow mast cells express IL-5Rα on their surface. A representative histogram depicts surface expression of IL-5Rα on bone marrow mast cells (left panel) and eosinophils (middle panel) from a patient with KIT D816V-positive SM. IL-5Rα surface expression was not detected on the patient’s bone marrow lymphocytes (right panel). The area under the curve for the isotype control (IgG-PE) is indicated in gray and for IL-5Rα (CDw125-PE) in white. Journal of Allergy and Clinical Immunology 2011 128, 1086-1092.e3DOI: (10.1016/j.jaci.2011.05.032) Copyright © 2011 Terms and Conditions

Fig E2 Human bone marrow mast cells express mRNA for sIL5Rα. Total RNA was isolated from human fibroblasts (ATCC cell line CCL-202), flow-sorted human bone marrow mast cells from a patient with SM, and purified human peripheral blood eosinophils reverse-transcribed and amplified using primers specific for sIL5Rα and β-actin as described in the Methods section. The PCR products were run on a 2% agarose gel and visualized with ethidium bromide. NTC, No template control. Journal of Allergy and Clinical Immunology 2011 128, 1086-1092.e3DOI: (10.1016/j.jaci.2011.05.032) Copyright © 2011 Terms and Conditions