Two novel serum biomarkers for endometriosis screened by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and their change.

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Two novel serum biomarkers for endometriosis screened by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and their change after laparoscopic removal of endometriosis Jianhong Jing, M.D., Yuhuan Qiao, M.D., Hiroshi Suginami, Ph.D., Fumiaki Taniguchi, M.D., Huirong Shi, Ph.D., Xiuli Wang, M.S. Fertility and Sterility Volume 92, Issue 4, Pages 1221-1227 (October 2009) DOI: 10.1016/j.fertnstert.2008.08.078 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (a) Peak intensity of proteins analyzed with Biomarker Wizard software. Diamond (S1) represents group of endometriosis patients, square (S5) represents control group. Peaks of 34 of 98 proteins were significantly different between the two groups. (b) Serum proteomic pattern in endometriosis and control samples with mass spectra (left) and gel view (right) of SELDI analysis. Two serum proteins were screened and selected as potential biomarkers of endometriosis by SELDI-TOF-MS. Fertility and Sterility 2009 92, 1221-1227DOI: (10.1016/j.fertnstert.2008.08.078) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (a) Protein intensities analyzed by Biomarker Wizard software. Diamond (S1) represents preoperative endometriosis group, square (S4) represents postoperative endometriosis group. Thirty potential protein masses were screened from 47 proteins peaks in preoperative endometriosis patients (P<.05). (b) Serum proteomic pattern of SELDI analysis in preoperative and postoperative samples with mass spectra (left) and gel view (right). The mass spectrographic profiles reveal an up-regulation of m/z 5830 peak in preoperative samples. Fertility and Sterility 2009 92, 1221-1227DOI: (10.1016/j.fertnstert.2008.08.078) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Serum protein content at 5,830 and 8,865 Da in four groups. For 5,830 Da and 8,865 Da: (3) vs. (2) and (3) vs. (1) and (3) vs. (4) P<.05; (1) vs. (4) P>.05. Note: Values are means ± SD. Fertility and Sterility 2009 92, 1221-1227DOI: (10.1016/j.fertnstert.2008.08.078) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Serum protein content at 5,830 and 8,865 Da in four stages. From stage I to stage IV, serum protein content at m/z 5,830 and m/z 8,865 had a trend of increase; however, there were no difference between stage I-II and stage III-IV (P>.05). Note: Values are means ± SD. Fertility and Sterility 2009 92, 1221-1227DOI: (10.1016/j.fertnstert.2008.08.078) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions