Modulation of the expression of tissue plasminogen activator and its inhibitor by hypoxia in human peritoneal and adhesion fibroblasts  Ghassan M Saed, Ph.D., Michael P Diamond, M.D.  Fertility and Sterility  Volume 79, Issue 1, Pages 164-168 (January 2003) DOI: 10.1016/S0015-0282(02)04557-0

FIGURE 1 Multiplex RT-PCR of tPA and β-actin in adhesion and peritoneal fibroblasts under normal and hypoxic conditions. (A), Multiplex RT-PCR of tPA and β-actin was performed as described in the methods section using total RNA isolated from normal peritoneal fibroblasts before (lane 1) and after hypoxia (lane 2) and adhesion fibroblasts before (lane 3) and after hypoxia (lane 4) treatment. Polymerase chain reaction products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. (B), The density of each band was measured by a scanning densimeter. Relative pixel densities corrected to internal control (β-actin) were used to compare the effect of hypoxia treatment on tPA mRNA levels. Error bars represent the standard error of the mean of triplicates. Results are representative of three independent experiments (P<.05). Gray, control; black, hypoxic. M = molecular weight marker. Saed. tPA and PAI-I expression by peritoneal fibroblasts. Fertil Steril 2003. Fertility and Sterility 2003 79, 164-168DOI: (10.1016/S0015-0282(02)04557-0)

FIGURE 2 Multiplex RT-PCR of PAI-I and β-actin in adhesion and peritoneal fibroblasts under normal and hypoxic conditions. (A), Multiplex RT-PCR of PAI-I and β-actin was performed as described in the methods section using total RNA isolated from normal peritoneal fibroblasts before (lane 1) and after hypoxia (lane 2) and adhesion fibroblasts before (lane 3) and after hypoxia (lane 4) treatment. Polymerase chain reaction products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. (B), The density of each band was measured by a scanning densimeter. Relative pixel densities corrected to internal control (β-actin) were used to compare the effect of hypoxia treatment on PAI-I mRNA levels. Error bars represent the standard error of the mean of triplicates. Results are representative of three independent experiments (P<.05). Gray, control; black, hypoxic. Saed. tPA and PAI-I expression by peritoneal fibroblasts. Fertil Steril 2003. Fertility and Sterility 2003 79, 164-168DOI: (10.1016/S0015-0282(02)04557-0)

FIGURE 3 tPA–PAI-I mRNA ratio in adhesion and peritoneal fibroblasts under normal and hypoxic conditions. The mRNA ratio for tPA and PAI-I were calculated from Figures 1 and 2 for normal peritoneal and adhesion fibroblasts, under normal and hypoxic conditions. Gray, control; black, hypoxic. Saed. tPA and PAI-I expression by peritoneal fibroblasts. Fertil Steril 2003. Fertility and Sterility 2003 79, 164-168DOI: (10.1016/S0015-0282(02)04557-0)