Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech.

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Genotypic characterisation of vancomycin-resistant Enterococcus faecium isolates from haemato-oncological patients at Olomouc University Hospital, Czech Republic M. Kolar, R. Pantucek, I. Vagnerova, M. Kesselova, P. Sauer, I. Matouskova, J. Doskar, D. Koukalova, P. Hejnar Clinical Microbiology and Infection Volume 12, Issue 4, Pages 353-360 (April 2006) DOI: 10.1111/j.1469-0691.2006.01365.x Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Representative SmaI macrorestriction fragment patterns from VanA+ Enterococcus faecium isolates. Clinical Microbiology and Infection 2006 12, 353-360DOI: (10.1111/j.1469-0691.2006.01365.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Dendrogram showing the similarity levels of the SmaI macrorestriction patterns of 59 VanA+ Enterococcus faecium isolates. The isolates are designated by capital letters indicating their isolation source (R, rectal swabs; U, urine; S, sputum; B, blood; H, hospital environment), together with a number/year of isolation. Two predominant clonal types with cut-off values of 95% similarity are indicated by vertical lines. SmaI pulsed-field gel electrophoresis (PFGE) types and subtypes of Tn1546-like elements obtained by ClaI long PCR restriction fragment length polymorphism analysis are indicated by Roman numerals (NT, non-typeable). Antibiograms (V, resistance to vancomycin; E, resistance to erythromycin; A, resistance to ampicillin; F, resistance to nitrofurantoin; T, resistance to tetracycline; C, resistance to chloramphenicol; S, high-level resistance to streptomycin; and G, high-level resistance to gentamicin) are indicated on the right. Clinical Microbiology and Infection 2006 12, 353-360DOI: (10.1111/j.1469-0691.2006.01365.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions