Chandra Goparaju, PhD, Jessica S

Slides:

Advertisements

Similar presentations

Massive Bronchogenic Cyst Joseph B. Levin, BA, Chi Wan Koo, MD, Jessica S. Donington, MD Journal of Thoracic Oncology Volume 5, Issue 11, Pages

Advertisements

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

Pathobiological Implications of MUC4 in Non–Small-Cell Lung Cancer

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Overexpression of CRM1: A Characteristic Feature in a Transformed Phenotype of Lung Carcinogenesis and a Molecular Target for Lung Cancer Adjuvant Therapy

MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.

INHBA Overexpression Promotes Cell Proliferation and May Be Epigenetically Regulated in Esophageal Adenocarcinoma Christopher W. Seder, MD, Wibisono.

Elevated FOXC2 Expression Promotes Invasion of HCC Cell Lines and is Associated with Poor Prognosis in Hepatocellular Carcinoma Cell Physiol Biochem 2017;44:99–109.

Volume 139, Issue 6, Pages e12 (December 2010)

Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling Baoping Cao, MD, PhD, Weili Yang,

Modulation of growth in human esophageal adenocarcinoma cells by group IIa secretory phospholipase A2 David Mauchley, MD, Xianzhong Meng, MD, PhD, Thomas.

Group IIa secretory phospholipase expression correlates with group IIa secretory phospholipase inhibition–mediated cell death in K-ras mutant lung cancer.

The role of Dickkopf-3 overexpression in esophageal adenocarcinoma

Prevention of lung ischemia–reperfusion injury by short hairpin RNA–mediated caspase- 3 gene silencing Yong-Xing Zhang, MD, Hong Fan, PhD, Yu Shi, MD,

WT1 Promotes Invasion of NSCLC via Suppression of CDH1

SIRT1 Regulates the Chemoresistance and Invasiveness of Ovarian Carcinoma Cells David Hamisi Mvunta, Tsutomu Miyamoto, Ryoichi Asaka, Yasushi Yamada,

Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.

Knockdown of secretory phospholipase A2 IIa reduces lung cancer growth in vitro and in vivo Jessica A. Yu, MD, David Mauchley, MD, Howard Li, MD, Xianzhong.

AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma Daniel L. Albertus, BS, Christopher.

INHBA Overexpression Promotes Cell Proliferation and May Be Epigenetically Regulated in Esophageal Adenocarcinoma Christopher W. Seder, MD, Wibisono.

Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT Zhen-Yu He, San-Gang Wu, Fang Peng,

SDHB deficiency promotes TGFβ-mediated invasion and metastasis of colorectal cancer through transcriptional repression complex SNAIL1-SMAD3/4 Haiyu Wang,

A Novel IMP1 Inhibitor, BTYNB, Targets c-Myc and Inhibits Melanoma and Ovarian Cancer Cell Proliferation Lily Mahapatra, Neal Andruska, Chengjian Mao,

MicroRNA-489 Plays an Anti-Metastatic Role in Human Hepatocellular Carcinoma by Targeting Matrix Metalloproteinase-7 Yixiong Lin, Jianjun Liu, Yuqi Huang,

Characterization of microRNA transcriptome in tumor, adjacent, and normal tissues of lung squamous cell carcinoma Jun Wang, MD, PhD, Zhi Li, MD, PhD,

Chandra M.V. Goparaju, PhD Journal of Thoracic Oncology

TGM2: A Cell Surface Marker in Esophageal Adenocarcinomas

Silencing of Discoidin Domain Receptor-1 (DDR1) Concurrently Inhibits Multiple Steps of Metastasis Cascade in Gastric Cancer Ryo Yuge, Yasuhiko Kitadai,

Volume 139, Issue 6, Pages e12 (December 2010)

Interleukin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage- Dominant Microenvironment in Lung Cancer Lunxu Liu, MD, PhD, Dongxia.

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

Identification and Validation of Long Noncoding RNA Biomarkers in Human Non–Small- Cell Lung Carcinomas Hui Yu, MD, Qinghua Xu, PhD, Fang Liu, PhD, Xun.

Characterization of Fibroblast Growth Factor Receptor 1 in Small-Cell Lung Cancer Anish Thomas, MD, Jih-Hsiang Lee, MD, Zied Abdullaev, PhD, Kang-Seo.

Justin D. Blasberg, MD, Chandra M. Goparaju, PhD, Harvey I

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Molecular Therapy - Nucleic Acids

Oncogenic Potential of CYP24A1 in Lung Adenocarcinoma

The Long Noncoding MALAT-1 RNA Indicates a Poor Prognosis in Non-small Cell Lung Cancer and Induces Migration and Tumor Growth Lars Henning Schmidt,

Overexpression of Muscarinic Receptor 3 Promotes Metastasis and Predicts Poor Prognosis in Non–Small-Cell Lung Cancer Gengpeng Lin, MD, Longhua Sun,

Activated Leukocyte Cell-Adhesion Molecule (ALCAM) Promotes Malignant Phenotypes of Malignant Mesothelioma Futoshi Ishiguro, MD, Hideki Murakami, MD,

Yuen Yee Cheng, PhD, Michaela B

Combining the Multitargeted Tyrosine Kinase Inhibitor Vandetanib with the Antiestrogen Fulvestrant Enhances Its Antitumor Effect in Non-small Cell Lung.

Frequent Coamplification and Cooperation between C-MYC and PVT1 Oncogenes Promote Malignant Pleural Mesothelioma Erick Riquelme, PhD, Milind B. Suraokar,

Thyroid Transcription Factor-1 Amplification and Expressions in Lung Adenocarcinoma Tissues and Pleural Effusions Predict Patient Survival and Prognosis

Volume 144, Issue 5, Pages e4 (May 2013)

Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.

Uc.454 Inhibited Growth by Targeting Heat Shock Protein Family A Member 12B in Non- Small-Cell Lung Cancer Jun Zhou, Chenghai Wang, Weijuan Gong, Yandan.

Myometrial cells undergo fibrotic transformation under the influence of transforming growth factor β-3 Doina S. Joseph, M.S., Minnie Malik, Ph.D., Sahadat.

Inhibiting MDM2-p53 Interaction Suppresses Tumor Growth in Patient-Derived Non– Small Cell Lung Cancer Xenograft Models Josephine Hai, PhD, Shingo Sakashita,

Aromatase Inhibitor Exemestane has Antiproliferative Effects on Human Mesothelioma Cells Daniela Stoppoloni, BSc, Luisa Salvatori, BSc, Annamaria Biroccio,

Molecular Therapy - Nucleic Acids

Prognostic Impact of Fibroblast Growth Factor 2 in Non-small Cell Lung Cancer: Coexpression with VEGFR-3 and PDGF-B Predicts Poor Survival Tom Donnem,

Vascular Endothelial Growth Factor (VEGF) Pathway

MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2 Ji-guang Zhang,

Frequency of Use and Predictors of Cancer-Directed Surgery in the Management of Malignant Pleural Mesothelioma in a Community-Based (Surveillance, Epidemiology,

Lung cancer cell invasion and expression of intercellular adhesion molecule-1 (ICAM-1) are attenuated by secretory phospholipase A2 inhibition Jessica.

Apelin Expression in Human Non-small Cell Lung Cancer: Role in Angiogenesis and Prognosis Judit Berta, MS, Istvan Kenessey, MD, Judit Dobos, PhD, Jozsef.

Laura S. Bisogno, Matthew B. Friedersdorf, Jack D. Keene

Increased Vascular-Endothelial Growth Factor (VEGF) Tumor Expression and Response to Epidermal Growth Factor Receptor (EGF-R) Inhibitor Erlotinib in Non-small.

Upregulation of Hypoxia-Inducible Factor-1α mRNA and its Clinical Significance in Non- small Cell Lung Cancer Tomofumi Yohena, MD, Ichiro Yoshino, MD,

Pathobiological Implications of MUC4 in Non–Small-Cell Lung Cancer

Kun-Peng Zhu, Xiao-Long Ma, Chun-Lin Zhang Molecular Therapy

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

MicroRNAs Dysregulation in Human Malignant Pleural Mesothelioma

Volume 17, Issue 2, Pages (February 2009)

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

Long Noncoding RNA BC as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3 Jiaxin Lin, Xin Tan,

The Expression of MicroRNA-598 Inhibits Ovarian Cancer Cell Proliferation and Metastasis by Targeting URI Feng Xing, Shuo Wang, Jianhong Zhou Molecular.

Overexpression of Muscarinic Receptor 3 Promotes Metastasis and Predicts Poor Prognosis in Non–Small-Cell Lung Cancer Gengpeng Lin, MD, Longhua Sun,

Overexpression of CRM1: A Characteristic Feature in a Transformed Phenotype of Lung Carcinogenesis and a Molecular Target for Lung Cancer Adjuvant Therapy

Presentation transcript:

Overexpression of EPH Receptor B2 in Malignant Mesothelioma Correlates with Oncogenic Behavior Chandra Goparaju, PhD, Jessica S. Donington, MD, Tsungda Hsu, PhD, Ryan Harrington, BS, Nathalie Hirsch, BS, Harvey I. Pass, MD Journal of Thoracic Oncology Volume 8, Issue 9, Pages 1203-1211 (September 2013) DOI: 10.1097/JTO.0b013e31829ceb6a Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 1 EPHB2 is overexpressed in MM tumors and cell lines. A, Affymetrix HG 1.0ST microarrays reveals a 3.75-fold (p = 2.39E-05) increase in EPHB2 gene-expression levels in tumors compared with matched normal peritoneum. B, Western blot analysis of tissue lysates also demonstrates overexpression of EPHB2 in clinical MM tumor specimens compared with matched normal peritoneum. C, Semiquantitative RT-PCR analysis reveals elevated levels of EPHB2 in MM cell lines compared with benign cell lines. PPIA, peptidylproplyl isomerase; EPHB2, ephrin B2 receptor; MM, malignant mesothelioma; NYU, New York University. Journal of Thoracic Oncology 2013 8, 1203-1211DOI: (10.1097/JTO.0b013e31829ceb6a) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 2 Targeted knockdown of EPHB2 with shRNA results in a significant decrease in EPHB2 protein expression (A) in H2595 and HP-1 cells. B, Immunofluorescence studies demonstrate decreased EPHB2 expression in H2595 and HP-1 MM cells after expression of its specific shRNA. EPHB2, ephrin B2 receptor. Journal of Thoracic Oncology 2013 8, 1203-1211DOI: (10.1097/JTO.0b013e31829ceb6a) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 3 A, qRT-PCR and Western blot analysis of EPHB2 downstream genes: Targeted knockdown of EPHB2 expression by its shRNA causes an increased expression of caspase 1 and caspase 8 mRNA (A) and protein (B). A decreased expression of BCL2L1, BIRC5, matrix metalloproteinase, and vascular endothelial growth factor mRNA (A) and protein (B) is noticed in H2595 and HP-1 cells. C, Caspase-8 activity: A significant increase in the caspase-8 activity in apoptotic cells is seen after forced expression of EPHB2-shRNA in H2595 and HP-1 cells compared with scrambled control. Peptidylproplyl isomerase A gene was used as a normalization control in both semi and qRT-PCR analysis. EPHB2, ephrin B2 receptor; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; EPHB2, ephrin B2 receptor. Journal of Thoracic Oncology 2013 8, 1203-1211DOI: (10.1097/JTO.0b013e31829ceb6a) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 4 Biological significance of EPHB2 in MM is demonstrated in in vitro functional assays after suppression of EPHB2 expressions by its shRNA. A, Cell-proliferation assay: a significant decrease in cell proliferation with a fold change of 1.4-fold to 2.0-fold (p < 0.001) was noticed in both H2595 and HP-1 cells. B, Scratch closure assay: a reduction in horizontal migration by 29% (p < 0.0005) in H2595 and 25% (p < 0.0005) in HP-1 is noticed. C, Cell invasion is significantly decreased in H2595 by 2.3-fold to 2.7-fold (p < 2.00E-05) and in HP-1 by 1.5-fold to 1.7 fold (p < 2.00E-05) compared with scrambled controls. D, Colony formation: significant decrease in the size and number of colonies was noted in both H2595 and HP-1 MM cells. However, no significant changes were noticed in LP9 cells. EPHB2, ephrin B2 receptor, MM, malignant mesothelioma. Journal of Thoracic Oncology 2013 8, 1203-1211DOI: (10.1097/JTO.0b013e31829ceb6a) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 5 Blocking peptide and antibody experiments in H2595 and HP-1 cells show a significant decrease in cell proliferation (A) and invasion (B). EPHB2, ephrin B2 receptor. Journal of Thoracic Oncology 2013 8, 1203-1211DOI: (10.1097/JTO.0b013e31829ceb6a) Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions