Decellularized vein as a potential scaffold for vascular tissue engineering Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M.

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Presentation transcript:

Decellularized vein as a potential scaffold for vascular tissue engineering Patrick J Schaner, MD, Niels D Martin, MD, Thomas N Tulenko, PhD, Irving M Shapiro, PhD, Nicholas A Tarola, MD, Rhoda F Leichter, MD, R.Anthony Carabasi, MD, Paul J DiMuzio, MD Journal of Vascular Surgery Volume 40, Issue 1, Pages 146-153 (July 2004) DOI: 10.1016/j.jvs.2004.03.033

Fig 1 Light micrograph of human greater saphenous vein before (left) and after (right) treatment with 0.075% SDS (hematoxylin-eosin stain, 20× original magnification). SDS treatment completely removes the luminal endothelial cells (arrow) and >94% of the cells within the vessel wall. Journal of Vascular Surgery 2004 40, 146-153DOI: (10.1016/j.jvs.2004.03.033)

Fig 2 Scanning electron micrograph of luminal surface of human greater saphenous vein before (left) and after (right) treatment with 0.075% SDS (500×). Endothelial cells seen on the left (arrow) are completely absent on the right, where only basement membrane is seen. Journal of Vascular Surgery 2004 40, 146-153DOI: (10.1016/j.jvs.2004.03.033)

Fig 3 Light micrograph of human greater saphenous vein before (left) and after (right) treatment with 0.075% SDS (Verhoff-vonGieson stain, 40× original magnification) revealing preservation of collagen (pink fibers, arrows) and elastin (black fibers, arrowheads) morphology. Journal of Vascular Surgery 2004 40, 146-153DOI: (10.1016/j.jvs.2004.03.033)

Fig 4 Quantification of area occupied by collagen (left) and elastin (right) fibers in human greater saphenous vein before and after treatment with 0.075% SDS, suggesting preservation of collagen and a slight decrease in elastin content. Values represent average fiber area per hpf ± SD (n = 10); *P < .02 compared with fresh vein. Journal of Vascular Surgery 2004 40, 146-153DOI: (10.1016/j.jvs.2004.03.033)

Fig 5 Basement membrane architecture of human greater saphenous vein before (left) and after (right) treatment with 0.075% SDS (200×). Immunohistochemistry using monoclonal antibody to human collagen Type IV stains the basement membrane brown, and reveals preservation of this protein along the luminal surface (arrow) as well as surrounding the areas formally occupied by the smooth muscle cells (arrowhead). Journal of Vascular Surgery 2004 40, 146-153DOI: (10.1016/j.jvs.2004.03.033)

Fig 6 In vitro strength studies measuring both burst pressure (left) and suture-holding (right) strength in human greater saphenous vein before and after cell extraction with 0.075% SDS. No significant alteration in strength was noted following decellularization. Values represent average ± SD (n = 10). Journal of Vascular Surgery 2004 40, 146-153DOI: (10.1016/j.jvs.2004.03.033)