Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling Eun-Sil Park, Ph.D., Dori C. Woods, Ph.D.,

Slides:

Advertisements

Similar presentations

Human chorionic gonadotropin controls luteal vascular permeability via vascular endothelial growth factor by down-regulation of a cascade of adhesion.

Advertisements

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Rong Hu, Ph. D. , Fei-miao Wang, M. D. , Liang Yu, Ph. D. , Yan Luo, M

Differential expression of G-protein-coupled estrogen receptor-30 in human myometrial and uterine leiomyoma smooth muscle Ruijuan Tian, M.Sc., Zengyong.

Transient expression of progesterone receptor and cathepsin-l in human granulosa cells during the periovulatory period Víctor García, M.D., Ph.D., Paulina.

Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus T. Nishida,

Cryoprotectants up-regulate expression of mouse oocyte AQP7, which facilitates water diffusion during cryopreservation Ya-Jing Tan, M.Sci., Yun Xiong,

Expression of neurotrophin 3 and its tropomyosin-related kinase receptor C in human preantral follicles Galia Oron, M.D., Asangla Ao, Ph.D., Or Friedman,

Lapatinib inhibits meiotic maturation of porcine oocyte-cumulus complexes cultured in vitro in gonadotropin-supplemented medium Eva Nagyova, D.V.M.,

2-Methoxyestradiol causes functional repression of transforming growth factor β3 signaling by ameliorating Smad and non-Smad signaling pathways in immortalized.

Inhibition of mammalian target of rapamycin signaling pathway decreases retinoic acid stimulated gene 8 expression in adult mouse testis Pinar Sahin,

Resistin decreases insulin-like growth factor I–induced steroid production and insulin- like growth factor I receptor signaling in human granulosa cells

The WNT/β-catenin signaling pathway and expression of survival promoting genes in luteinized granulosa cells: endometriosis as a paradigm for a dysregulated.

Masanori Ono, M. D. , Ph. D. , Ping Yin, Ph. D. , Antonia Navarro, M

Comparative gene expression profiling of adult mouse ovary-derived oogonial stem cells supports a distinct cellular identity Anthony N. Imudia, M.D.,

The impact of culture conditions on early follicle recruitment and growth from human ovarian cortex biopsies in vitro Jana Liebenthron, M.Sc., Maria.

Chu-Chao Zhu, B. Sc. , Hua Zhang, M. D. , Jin-Shan Zhang, M. D

Ming-hui Chen, M. D. , Tao Li, Ph. D. , Chen-hui Ding, Ph. D

Interleukin-17A is present in neutrophils in endometrioma and stimulates the secretion of growth-regulated oncogene–α (Gro-α) from endometrioma stromal.

Mesenchymal stem cells derived from Wharton jelly of the human umbilical cord ameliorate damage to human endometrial stromal cells Xiaoqing Yang, M.S.,

Androstenedione induces abnormalities in morphology and function of developing oocytes, which impairs oocyte meiotic competence Wataru Tarumi, M.Sc.,

Dienogest enhances autophagy induction in endometriotic cells by impairing activation of AKT, ERK1/2, and mTOR JongYeob Choi, Ph.D., MinWha Jo, M.S.,

Uterine luminal epithelium–specific proline-rich acidic protein 1 (PRAP1) as a marker for successful embryo implantation Honglu Diao, Ph.D., Shuo Xiao,

Comparative evaluation of different in vitro systems that stimulate germ cell differentiation in human embryonic stem cells Mark Richards, Ph.D., Chui-Yee.

Dienogest inhibits nerve growth factor expression induced by tumor necrosis factor-α or interleukin-1β Shizuka Mita, M.S., Yutaka Shimizu, Ph.D., Ayumi.

The antifibrotic drug halofuginone inhibits proliferation and collagen production by human leiomyoma and myometrial smooth muscle cells Meagan M. Grudzien,

Regine Gaetje, M. D. , Uwe Holtrich, Ph. D. , Thomas Karn, Ph. D

In vitro effects of phthalate esters in human myometrial and leiomyoma cells and increased urinary level of phthalate metabolite in women with uterine.

Endometrial expression and in vitro modulation of the iron transporter divalent metal transporter-1: implications for endometriosis Carlos Patricio Alvarado-Díaz,

Expression of growth differentiation factor-9 and bone morphogenetic protein-15 in oocytes and cumulus granulosa cells of patients with polycystic ovary.

Transforming growth factor (TGF)-β1-induced human endometrial stromal cell decidualization through extracellular signal-regulated kinase and Smad activation.

Multidrug-resistant transport activity protects oocytes from chemotherapeutic agents and changes during oocyte maturation Lynae M. Brayboy, M.D., Nathalie.

Tumor necrosis factor α up-regulates endometrial milk fat globule–epidermal growth factor 8 protein production via nuclear factor κB activation, resulting.

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes Lu Zhang, M.Sc., Dan-Yu Lu, M.Sc., Wan-Yun.

Administration of visfatin during superovulation improves developmental competency of oocytes and fertility potential in aged female mice Kyoung-Hwa.

Leiomyoma-derived transforming growth factor-β impairs bone morphogenetic protein-2- mediated endometrial receptivity Leo F. Doherty, M.D., Hugh S. Taylor,

María Guillermina Bilbao, Ph. D. , María Paula Di Yorio, Ph. D

Sana M. Salih, M. D. , Salama A. Salama, Ph. D. , Amin A. Fadl, Ph. D

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Kisspeptin stimulates progesterone secretion via the Erk1/2 mitogen-activated protein kinase signaling pathway in rat luteal cells Jing Peng, M.D., Min.

Marisol Quezada, Ph. D. , Jikui Wang, Ph. D. , Valerie Hoang, M. S

Age-associated alteration of oocyte-specific gene expression in polar bodies: potential markers of oocyte competence Ze-Xu Jiao, M.D., Ph.D., Min Xu,

Aquaporin 7 expression in postimplantation mouse uteri: a potential role for glycerol transport in uterine decidualization Hongying Peng, M.D., Ying.

Estrogenic regulation of testicular expression of stem cell factor and c-kit: implications in germ cell survival and male fertility Sara Correia, M.S.,

Leukemia inhibitory factor increases the invasiveness of trophoblastic cells through integrated increase in the expression of adhesion molecules and pappalysin.

Yan Wen, M. D. , Rudy Quintero, M. D. , Bertha Chen, M. D

Estrogen receptor β agonist diarylpropionitrile inhibits lipopolysaccharide-induced regulated on activation normal T cell expressed and secreted (RANTES)

Mu opioid receptor in the human endometrium: dynamics of its expression and localization during the menstrual cycle Lide Totorikaguena, B.S., Estibaliz.

Dynamics of expression and localization of the cannabinoid system in granulosa cells during oocyte nuclear maturation Ekaitz Agirregoitia, Ph.D., Inés.

Volume 122, Issue 2, Pages (July 2005)

Expression of bone morphogenetic proteins 4 and 7 and their receptors IA, IB, and II in human ovaries from fetuses and adults Ronit Abir, Ph.D., Avi.

Sima Margulis, Ronit Abir, Ph. D. , Carmela Felz, Shmuel Nitke, M. D

Testosterone dependent androgen receptor stabilization and activation of cell proliferation in primary human myometrial microvascular endothelial cells

Bita Nickkholgh, M. D. , Sefika Canan Mizrak, V. M. D. , Ph. D

Requirement of heart and neural crest derivatives–expressed transcript 2 during decidualization of human endometrial stromal cells in vitro Hisayuu Shindoh,

Cryopreservation of testicular tissue before long-term testicular cell culture does not alter in vitro cell dynamics Yoni Baert, M.Sc., Aude Braye, M.Sc.,

Aromatase expression in abdominal omental/visceral and subcutaneous fat depots: a comparison of pregnant and obese women Suman Rice, Ph.D., Bijal Patel,

Glial cell line–derived neurotrophic factor (GDNF) and its receptors in human ovaries from fetuses, girls, and women Jacob Farhi, M.D., Asangla Ao, Ph.D.,

Activin Signals through SMAD2/3 to Increase Photoreceptor Precursor Yield during Embryonic Stem Cell Differentiation Amy Q. Lu, Evgenya Y. Popova, Colin.

Antimüllerian hormone inhibits follicle-stimulating hormone-induced adenylyl cyclase activation, aromatase expression, and estradiol production in human.

Bone morphogenetic protein-6 stimulates gene expression of follicle-stimulating hormone receptor, inhibin/activin β subunits, and anti-Müllerian hormone.

Sorafenib inhibits growth, migration, and angiogenic potential of ectopic endometrial mesenchymal stem cells derived from patients with endometriosis

Histone deacetylase inhibitors down-regulate G-protein-coupled estrogen receptor and the GPER-antagonist G-15 inhibits proliferation in endometriotic.

Fertility and Sterility

Effects of hyperglycemia on the differential expression of insulin and insulin-like growth factor-I receptors in human normal peritoneal and adhesion.

Lipopolysaccharide-promoted proliferation of endometriotic stromal cells via induction of tumor necrosis factor α and interleukin-8 expression Yumiko.

Daniela Zuccarello, Ph. D. , Andrea Garolla, Ph. D

Resveratrol inhibits the mevalonate pathway and potentiates the antiproliferative effects of simvastatin in rat theca-interstitial cells Donna H. Wong,

Bone morphogenetic protein 7 (BMP-7) increases the expression of follicle-stimulating hormone (FSH) receptor in human granulosa cells Jia Shi, M.D.,

Shao-Chen Sun, Ph. D. , Wei-Wei Gao, M. S. , Yong-Nan Xu, Ph. D

Presentation transcript:

Bone morphogenetic protein 4 promotes mammalian oogonial stem cell differentiation via Smad1/5/8 signaling Eun-Sil Park, Ph.D., Dori C. Woods, Ph.D., Jonathan L. Tilly, Ph.D. Fertility and Sterility Volume 100, Issue 5, Pages 1468-1475.e2 (November 2013) DOI: 10.1016/j.fertnstert.2013.07.1978 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Detection of Bmpr1a, Bmpr1b, and Bmpr2 mRNAs in cultured OSCs (no RT, PCR analysis of RNA sample without RT used as a negative control to exclude genomic DNA contamination; β-actin, internal sample loading control). (B–D) Representative immunofluorescence-based detection of BMPR1A, BMPR1B, and BMPR2 proteins (green) in cultured OSCs counterstained with DAPI (blue) to visualize nuclear DNA (scale bars, 10 μm). Fertility and Sterility 2013 100, 1468-1475.e2DOI: (10.1016/j.fertnstert.2013.07.1978) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 (A–C) Effect of BMP4 and Noggin, alone (A, B) or in combination (C), on numbers of IVD oocytes formed per well of 2.5 × 104 OSCs after 16 hours of treatment (mean ± SEM, n = 3–4 independent cultures; different letters, P<.05). (D) Lack of effect of BMP4 (100 ng/mL) and Noggin (150 ng/mL), alone or in combination, on proliferation of OSCs (2.5 × 104 cells initially seeded per well) over a 40-hour culture period (mean ± SEM, n = 4 independent cultures; no significant differences detected). Fertility and Sterility 2013 100, 1468-1475.e2DOI: (10.1016/j.fertnstert.2013.07.1978) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Representative immunoblot depicting the effect of BMP4 (100 ng/mL) and Noggin (150 ng/mL), alone or in combination, on Smad1/5/8 phosphorylation (p-Smad1/5/8) in wells of 5 × 104 OSCs after the indicated durations of treatment. Note that total Smad1 protein and β-actin protein levels remain comparable across treatment groups. Fertility and Sterility 2013 100, 1468-1475.e2DOI: (10.1016/j.fertnstert.2013.07.1978) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 4 (A) Conventional RT-PCR analysis of Stra8, Msx1, and Msx2 mRNA levels in OSCs (2.5 × 104 cells per well) treated for 24 hours without or with 100 ng/mL BMP4, alone or in combination with 150 ng/mL Noggin (no RT, PCR analysis of RNA sample without RT used as a negative control to exclude genomic DNA contamination; β-actin, internal sample loading control). (B) Representative examples of immunofluorescence-based detection of Stra8 protein (green, arrows) in cultured OSCs, counterstained with rhodamine phalloidin (red) and DAPI (blue) to visualize cytoplasmic F-actin and nuclear DNA, respectively (scale bars, 10 μm). (C–E) Real-time (quantitative) RT-PCR analysis of changes in Stra8 (C), Msx1 (D), and Msx2 (E) mRNA levels normalized to β-actin mRNA levels in OSCs (2.5 × 104 cells per well) treated for 24 hours without or with 100 ng/mL BMP4, alone or in combination with 150 ng/mL Noggin (mean ± SEM, n = 4 independent cultures; different letters, P<.05). Fertility and Sterility 2013 100, 1468-1475.e2DOI: (10.1016/j.fertnstert.2013.07.1978) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Supplemental Figure 1 (A) Morphology of IVD oocytes generated by OSCs cultured without (vehicle, Veh) or with 100 ng/mL BMP4. (B) Comparative gene expression analysis of markers for germ cells (Ddx4) and oocytes (Ybx2, Nobox, Zp1, Zp2, Zp3, Gdf9) in IVD oocytes collected from cultures of OSCs treated with vehicle (Veh) or 100 ng/mL BMP4 (no RT, PCR analysis of RNA sample without RT used as a negative control for genomic DNA contamination; β-actin, internal sample loading control). Fertility and Sterility 2013 100, 1468-1475.e2DOI: (10.1016/j.fertnstert.2013.07.1978) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions