Direct comparison of efficiency and stability of gene transfer into the mammalian heart using adeno-associated virus versus adenovirus vectors Danny.

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Presentation transcript:

Direct comparison of efficiency and stability of gene transfer into the mammalian heart using adeno-associated virus versus adenovirus vectors Danny Chu, MD, Christopher C Sullivan, MS, Matthew D Weitzman, PhD, Lingling Du, MD, Paul L Wolf, MD, Stuart W Jamieson, MB, FRCS, Patricia A Thistlethwaite, MD, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 126, Issue 3, Pages 671-679 (September 2003) DOI: 10.1016/S0022-5223(03)00082-5

Figure 1 Gross photographs of rat hearts cut axially at various time points after gene delivery (X-gal stained for 24 hours, magnification ×2). Adeno, Adeno-lacZ–injected hearts; AAV, AAV-lacZ–injected hearts. The Journal of Thoracic and Cardiovascular Surgery 2003 126, 671-679DOI: (10.1016/S0022-5223(03)00082-5)

Figure 2 Western blots of β-galactosidase and actin filament protein expression from rat hearts at various time points after gene delivery. Actin, Actin filament protein; LacZ, β-galactosidase protein; d, day; kD, kilodalton; m, month; Adeno-lacZ, Adeno-lacZ–injected hearts; AAV-lacZ, AAV-lacZ–injected hearts. The Journal of Thoracic and Cardiovascular Surgery 2003 126, 671-679DOI: (10.1016/S0022-5223(03)00082-5)

Figure 3 Beta-galactosidase activity assay of rat hearts after intramyocardial injection of AAV-lacZ or Adeno-lacZ at various time points. Data are expressed as the mean ± standard error of the mean (SEM). *P < .05 versus Adeno-lacZ group. Adeno, Adeno-lacZ–injected hearts; AAV, AAV-lacZ–injected hearts; d, day; m, month; nm, nanometer. The Journal of Thoracic and Cardiovascular Surgery 2003 126, 671-679DOI: (10.1016/S0022-5223(03)00082-5)

Figure 4 Western blots of β-galactosidase and actin filament protein expression from various rat organs 1 month after intramyocardial injection of AAV-lacZ and 7 days after intramyocardial injection of Adeno-lacZ. Actin, Actin filament protein; kD, kilodalton; LacZ, β-galactosidase protein. The Journal of Thoracic and Cardiovascular Surgery 2003 126, 671-679DOI: (10.1016/S0022-5223(03)00082-5)

Figure 5 Photomicrographs of rat hearts at 7 days and 1 month after intramyocardial injection (hematoxylin and eosin stained, X-gal stained, magnification ×20). Arrows denote examples of cardiomyocytes positive for β-galactosidase expression. Adeno, Adeno-lacZ–injected heart; AAV, AAV-lacZ–injected heart; PBS, phosphate-buffered saline–injected heart. The Journal of Thoracic and Cardiovascular Surgery 2003 126, 671-679DOI: (10.1016/S0022-5223(03)00082-5)