Volume 61, Issue 1, Pages S68-S72 (January 2002)

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Volume 61, Issue 1, Pages S68-S72 (January 2002) Glomerular β-galactosidase expression following transduction with microsphere- adenoviral complexes Udayan Y. Bhatt, Thomas J. Sferra, Amy Johnson, Caroline Williams, Kristen Shirey, Tammy Venema, Gerard J. Nuovo, N. Stanley Nahman Kidney International Volume 61, Issue 1, Pages S68-S72 (January 2002) DOI: 10.1046/j.1523-1755.2002.0610s1068.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 Kidney and liver sections demonstrating the patterns of histologic expression of the βgal transprotein from rats transduced with adenoviral-microsphere complexes (A and B) or free adenovirus (C and D). (A) Representative glomerulus demonstrating extensive βgal expression (blue pigment) (×400). (B) Representative liver section from the same rat showing a single transduced hepatocyte (×400). (C) Glomerulus showing no transprotein expression (no glomeruli from this rat exhibited any βgal activity). (D) Representative liver section demonstrating multiple foci of transduction (×100). Kidney International 2002 61, S68-S72DOI: (10.1046/j.1523-1755.2002.0610s1068.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 RT in situ PCR demonstrating expression of the βgal transgene in the glomerulus from a rat transduced with microspheres complexed to adenovirus containing a CMV-lacZ expression cassette. (A) Glomerular tuft showing diffuse expression of βgal mRNA (dense blue pigment). (B) Higher power view of (A) demonstrating βgal expression (white arrows) and pink cell nucleus (black arrow). (C) Control section probed with irrelevant primers. (D) Control section not subjected to DNAase I treatment. The expected diffuse blue pigmentation is evident (A, C, and D, ×1000; B, ×3000; MS, microsphere). Kidney International 2002 61, S68-S72DOI: (10.1046/j.1523-1755.2002.0610s1068.x) Copyright © 2002 International Society of Nephrology Terms and Conditions