Substantial Sex-Dependent Differences in the Response of Human Scalp Hair Follicles to Estrogen Stimulation In Vitro Advocate Gender-Tailored Management.

Slides:

Advertisements

Similar presentations

Vladimir A. Botchkarev, Jiro Kishimoto

Advertisements

Nestin in Human Skin: Exclusive Expression in Intramesenchymal Skin Compartments and Regulation by Leptin Stephan Tiede, Jennifer E. Kloepper, Nancy.

Female Pattern Hair Loss and its Relationship to Permanent/Cicatricial Alopecia: A New Perspective Elise A. Olsen Journal of Investigative Dermatology.

NF-κB Activity Is Required for Anagen Maintenance in Human Hair Follicles In Vitro Jennifer E. Kloepper, Nancy Ernst, Karsten Krieger, Enikő Bodó, Tamás.

Profiling the Response of Human Hair Follicles to Ultraviolet Radiation Zhongfa Lu, Tobias W. Fischer, Sybille Hasse, Koji Sugawara, York Kamenisch, Sven.

Human Female Hair Follicles Are a Direct, Nonclassical Target for Thyroid-Stimulating Hormone Enikö Bodó, Arno Kromminga, Tamás Bíró, István Borbíró,

Corticotropin-Releasing Hormone Stimulates the In Situ Generation of Mast Cells from Precursors in the Human Hair Follicle Mesenchyme Natsuho Ito, Koji.

Hypothalamic–Pituitary–Thyroid Axis Hormones Stimulate Mitochondrial Function and Biogenesis in Human Hair Follicles Silvia Vidali, Jana Knuever, Johannes.

Expression patterns of the glial cell line–derived neurotrophic factor, neurturin, their cognate receptors GFRα-1, GFRα-2, and a common signal transduction.

The Distribution of Estrogen Receptor β Is Distinct to That of Estrogen Receptor α and the Androgen Receptor in Human Skin and the Pilosebaceous Unit

István Borbíró, Erika Lisztes, Balázs I

Nathan J. Hawkshaw, Iain S. Haslam, David M

Kai Kretzschmar, Denny L. Cottle, Pawel J. Schweiger, Fiona M. Watt

The Peripheral Clock Regulates Human Pigmentation

A Meeting of Two Chronobiological Systems: Circadian Proteins Period1 and BMAL1 Modulate the Human Hair Cycle Clock Yusur Al-Nuaimi, Jonathan A. Hardman,

The Thyroid Hormone Analogue KB2115 (Eprotirome) Prolongs Human Hair Growth (Anagen) Ex Vivo Attila Oláh, Jennifer Gherardini, Marta Bertolini, Jérémy.

Hair Follicle Mesenchyme-Associated PD-L1 Regulates T-Cell Activation Induced Apoptosis: A Potential Mechanism of Immune Privilege Xiaojie Wang, Alexandra.

P-Cadherin Regulates Human Hair Growth and Cycling via Canonical Wnt Signaling and Transforming Growth Factor-β2 Liat Samuelov, Eli Sprecher, Daisuke.

Enhancing Structural Support of the Dermal Microenvironment Activates Fibroblasts, Endothelial Cells, and Keratinocytes in Aged Human Skin In Vivo Taihao.

James M. Waters, Jessica E. Lindo, Ruth M. Arkell, Allison J. Cowin

Differences in Expression of Specific Biomarkers Distinguish Human Beard from Scalp Dermal Papilla Cells Susan E. Rutberg, Meredith L. Kolpak, John A.

Analysis of Hair Follicles in Mutant Laboratory Mice

Estrogen Receptor α, but not Estrogen Receptor β, is Involved in the Regulation of the Hair Follicle Cycling as well as the Thickness of Epidermis in.

The mRNA for Protease Nexin-1 is Expressed in Human Dermal Papilla Cells and its Level is Affected by Androgen Tadashige Sonoda, Yuji Asada, Sotaro Kurata,

Estrogen Upregulates Slug to Enhance the Migration of Keratinocytes

Prevalence of Male and Female Pattern Hair Loss in Maryborough

Nestin in Human Skin: Exclusive Expression in Intramesenchymal Skin Compartments and Regulation by Leptin Stephan Tiede, Jennifer E. Kloepper, Nancy.

Vanilloid Receptor-1 (VR1) is Widely Expressed on Various Epithelial and Mesenchymal Cell Types of Human Skin Enikő Bodó, Ilona Kovács, Andrea Telek,

Tomoyasu Hattori, Lukasz Stawski, Sashidhar S

Plasticity of Rodent and Human Hair Follicle Dermal Cells:Implications for Cell Therapy and Tissue Engineering Gavin D. Richardson, Elisabeth C. Arnott,

Characterization of Human Keratin-Associated Protein 1 Family Members

Integrin β6-Deficient Mice Show Enhanced Keratinocyte Proliferation and Retarded Hair Follicle Regression after Depilation Yanshuang Xie, Kevin J. McElwee,

Topical Estrogen Accelerates Hair Regrowth in Mice After Chemotherapy-Induced Alopecia by Favoring the Dystrophic Catagen Response Pathway to Damage

Adenosine Stimulates Fibroblast Growth Factor-7 Gene Expression Via Adenosine A2b Receptor Signaling in Dermal Papilla Cells Masato Iino, Ritsuko Ehama,

NF-κB Activity Is Required for Anagen Maintenance in Human Hair Follicles In Vitro Jennifer E. Kloepper, Nancy Ernst, Karsten Krieger, Enikő Bodó, Tamás.

Thyrotropin-Releasing Hormone Selectively Stimulates Human Hair Follicle Pigmentation Erzsébet Gáspár, Kim T. Nguyen-Thi, Celine Hardenbicker, Stephan.

Immunology of the Human Nail Apparatus: The Nail Matrix Is a Site of Relative Immune Privilege Taisuke Ito, Natsuho Ito, Matthias Saathoff, Barbara Stampachiacchiere,

Sustained Activation of Fibroblast Transforming Growth Factor-β/Smad Signaling in a Murine Model of Scleroderma Shinsuke Takagawa, Gabriella Lakos, Yasuji.

Supriya Kapas, Paula M. Farthing Journal of Investigative Dermatology

Mitochondrial Function in Murine Skin Epithelium Is Crucial for Hair Follicle Morphogenesis and Epithelial–Mesenchymal Interactions Jennifer E. Kloepper,

High Invasive Melanoma Cells Induce Matrix Metalloproteinase-1 Synthesis in Fibroblasts by Interleukin-1α and Basic Fibroblast Growth Factor-Mediated.

The Role of Chronic Inflammation in Cutaneous Fibrosis: Fibroblast Growth Factor Receptor Deficiency in Keratinocytes as an Example Michael Meyer, Anna-Katharina.

Satoshi Itami, Shigeki Inui

Yuko Oda, Lizhi Hu, Vadim Bul, Hashem Elalieh, Janardan K

Fate of Prominin-1 Expressing Dermal Papilla Cells during Homeostasis, Wound Healing and Wnt Activation Grace S. Kaushal, Emanuel Rognoni, Beate M. Lichtenberger,

Advanced Inhibition of Undesired Human Hair Growth by PPARγ Modulation? Yuval Ramot, Arianna Mastrofrancesco, Erika Herczeg-Lisztes, Tamás Bíró, Mauro.

Distinct Roles for Nerve Growth Factor and Brain-Derived Neurotrophic Factor in Controlling the Rate of Hair Follicle Morphogenesis Natalia V. Botchkareva,

Towards Dissecting the Pathogenesis of Retinoid-Induced Hair Loss: All-Trans Retinoic Acid Induces Premature Hair Follicle Regression (Catagen) by Upregulation.

Protease-Activated Receptor 2, a Receptor Involved in Melanosome Transfer, is Upregulated in Human Skin by Ultraviolet Irradiation Glynis Scott, Cristina.

YAP and TAZ Regulate Skin Wound Healing

Journal of Investigative Dermatology

Dihydrotestosterone-Inducible IL-6 Inhibits Elongation of Human Hair Shafts by Suppressing Matrix Cell Proliferation and Promotes Regression of Hair Follicles.

Characterization of Human Keratin-Associated Protein 1 Family Members

Society for Investigative Dermatology 2010 Meeting Minutes

Distinct Roles for Nerve Growth Factor and Brain-Derived Neurotrophic Factor in Controlling the Rate of Hair Follicle Morphogenesis Natalia V. Botchkareva,

Hair Growth–Promoting Effects of Adiponectin In Vitro

Research Snippets Journal of Investigative Dermatology

A Potential Suppressor of TGF-β Delays Catagen Progression in Hair Follicles Yumiko Tsuji, Sumiko Denda, Tsutomu Soma, Laurel Raftery, Takashi Momoi,

Edar Signaling in the Control of Hair Follicle Development

James Gailit, Mary J. Marchese, Richard R. Kew, Barry L. Gruber

Thrombospondin-1 Plays a Critical Role in the Induction of Hair Follicle Involution and Vascular Regression During the Catagen Phase Kiichiro Yano, Michael.

MHC Class I Expression in Murine Skin: Developmentally Controlled and Strikingly Restricted Intraepithelial Expression During Hair Follicle Morphogenesis.

Vladimir A. Botchkarev, Jiro Kishimoto

Hair Breakage in Normal and Weathered Hair: Focus on the Black Patient

A Human Folliculoid Microsphere Assay for Exploring Epithelial– Mesenchymal Interactions in the Human Hair Follicle Blanka Havlickova, Tamás Bíró, Alessandra.

Marie-Louise T. Johnson

Liren Tang, Shabnam Madani, Harvey Lui, Jerry Shapiro

Estrogens and Human Scalp Hair Growth—Still More Questions than Answers Franziska Conrad, Ulrich Ohnemus, Enikö Bodo, Albrecht Bettermann, Ralf Paus

Mind the (Gender) Gap: Does Prolactin Exert Gender and/or Site-Specific Effects on the Human Hair Follicle? Ewan A. Langan, Yuval Ramot, Vincent Goffin,

Rolf Hoffmann, Shiro Niiyama Journal of Investigative Dermatology

Presentation transcript:

Substantial Sex-Dependent Differences in the Response of Human Scalp Hair Follicles to Estrogen Stimulation In Vitro Advocate Gender-Tailored Management of Female Versus Male Pattern Balding Franziska Conrad, Ulrich Ohnemus, Enikö Bodo, Tamas Biro, Birte Tychsen, Bernhard Gerstmayer, Andreas Bosio, Thomas Schmidt- Rose, Silke Altgilbers, Albrecht Bettermann, Matthias Saathoff, Wilfried Meyer, Ralf Paus Journal of Investigative Dermatology Symposium Proceedings Volume 10, Issue 3, Pages 243-246 (December 2005) DOI: 10.1111/j.1087-0024.2005.10115.x Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Sex-dependent differences in estrogen receptor type β (ER-β)-like immunoreactivity in human frontotemporal scalp hair follicles. ER-β-like immunoreactivity was found in (A) male microdissected frontotemporal scalp hair follicles predominantly in the matrix keratinocytes (MK), (B) in female hair follicles immunoreactivity was predominantly found in the dermal papilla (DP) fibroblasts (ABC staining, substrat DAB (diaminobenzadine) as chromogen, methylengreene-counterstaining, gray scale, and inverted, original magnification × 100). (C) Quantitative analysis of sex-dependent differences in ER-β-like immunoreactivity in the MK and DP was performed using Scion image (mean intensity), n=36 hair follicles per group, SEM. **0.001≤p≤0.01. Journal of Investigative Dermatology Symposium Proceedings 2005 10, 243-246DOI: (10.1111/j.1087-0024.2005.10115.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 17-β-estradiol (E2) significantly increases estrogen receptor type β (ER-β)-like immunoreactivity in male frontotemporal scalp hair follicles. Stimulation with E2 (10 nM) on organ-cultured male frontotemporal scalp hair follicles increased the immunoreactivity of ER-β 503 in the dermal papilla fibroblasts. Quantitative analysis using Scion image (mean intensity), SEM, *0.01≤p≤0.05. Journal of Investigative Dermatology Symposium Proceedings 2005 10, 243-246DOI: (10.1111/j.1087-0024.2005.10115.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Sex-dependent differences in 17-β-estradiol (E2)-responsive target genes in frontotemporal human scalp hair follicles. Suppression and stimulation of genes in human scalp hair follicles using a skin focus chip/microarray (white: female, black: male) after 48 h culture with E2 (10 nM). Bone morphogenetic protein 7 (BMP7), cysteine-rich 61 (angiogenic inducer), fibroblast growth factor receptor 2 (FGFR2), high endothelial venule protein (HEVIN), nuclear receptor subfamily4, groupA, member1 (NR4A1). For ratio list of all genes see Tables S1 and S2. Journal of Investigative Dermatology Symposium Proceedings 2005 10, 243-246DOI: (10.1111/j.1087-0024.2005.10115.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Transforming growth factor-β2 (TGF-β2)-like immunoreactivity expression pattern shows significant differences after 17-β-estradiol (E2) treatment in the lower outer root sheath (ORS) in male and female hair follicles. (A, B) TGF-β2-like immunoreactivity increases significantly in the lower ORS of (A) female scalp hair follicles and declines in the lower ORS of (B) male hair follicles after E2 treatment in vitro. Culture period 48 h (TSA staining, label rhodamine, nuclear counterstaining 4′,6-diamino-2-phenylindole (DAPI), gray scale and inverted, original magnification × 250). (C, D) Quantitative analysis of sex-dependent differences in TGF-β2-like immunoreactivity using Scion image (mean intensity), (C) female, (D) male, E2-treated mean intensity was compared with the non-treated control, n=36 hair follicles per group, SEM, p-value: 0.01<*<0.05, 0.001<**<0.01. Journal of Investigative Dermatology Symposium Proceedings 2005 10, 243-246DOI: (10.1111/j.1087-0024.2005.10115.x) Copyright © 2005 The Society for Investigative Dermatology, Inc Terms and Conditions