Targeted DNA vaccination induced protection against nasal challenge with influenza virus 14 d after DNA vaccination. Targeted DNA vaccination induced protection.

Slides:

Advertisements

Similar presentations

Immune Parameters Correlate with Protection Against Ebola Virus Infection in Rodents and Nonhuman Primates by Gary Wong, Jason S. Richardson, Stéphane.

Advertisements

Volume 5, Issue 3, Pages (March 2002)

Antitumor effect of the combination of IL-2 IC and anti–CTLA-4.

Identification of combination treatment–responsive dysfunctional tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

A Combinatorial CRISPR-Cas9 Attack on HIV-1 DNA Extinguishes All Infectious Provirus in Infected T Cell Cultures Gang Wang, Na Zhao, Ben Berkhout, Atze.

Serum IgG (A–D) and IgA (E–H) titer from BALB/c mice (n = 5/group) at day 0 or following COBRA P1 virus i.n., i.p., or i.m. prime at day 21 or following.

Volume 25, Issue 6, Pages (June 2017)

Antiviral activity of Chongkukjang extracts against influenza A virus in vitro and in vivo Bai Wei, Se-Yeoun Cha, Min Kang, Young Jin Kim, Chang-Won Cho,

Characterization of the targeting units within the vaccine proteins.

In vivo prophylactic and therapeutic efficacy of C12G6 in mice

Long-lasting protection against nasal challenge with influenza virus 252 d after a single DNA vaccination. Long-lasting protection against nasal challenge.

HA-specific Abs in serum 14 d after a single vaccination with the various targeted DNA vaccines. HA-specific Abs in serum 14 d after a single vaccination.

A single DNA vaccination of C57BL/6 mice induced Ab responses of IgG1 and/or IgG2c subclass. A single DNA vaccination of C57BL/6 mice induced Ab responses.

Fig. 7 Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors. Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors.

Virus-specific serum antibody response of BALB/c mice to DNA prime followed by VRP boost. Virus-specific serum antibody response of BALB/c mice to DNA.

Volume 26, Issue 2, Pages (February 2018)

Type 1 immunity drives metabolic disease but protects against NAFLD

Protection from influenza virus challenge.

Prf1−/− mice exhibit increased immunopathology with prior CD8 T cell memory secondary to immunization. Prf1−/− mice exhibit increased immunopathology with.

Viral Diseases How do vaccines work?.

Virus-specific serum antibody response of BALB/c mice to DNA prime followed by VRP boost. Virus-specific serum antibody response of BALB/c mice to DNA.

GM-CSF–producing γδT cells are involved in the development of EAM

Survival of BALB/c mice after HK/Syd challenge.

Therapeutic effects of TIP60 allosteric modification in DSS-induced colitis. Therapeutic effects of TIP60 allosteric modification in DSS-induced colitis.

Expression of NCoR1∆ID in PAX8−/− mice does not reverse repression of T3-positive target genes. qPCR on the indicated pituitary (A) and neuronal (B) genes.

IL-21 is involved in the pathogenesis of EAM

Serum neutralizing antibody response of BALB/c mice to DNA prime followed by VRP boost. Serum neutralizing antibody response of BALB/c mice to DNA prime.

Indomethacin worsens the effects of C. difficile infection in mice.

The psoriatic phenotype of DKO

Volume 25, Issue 9, Pages (September 2017)

Comparison of therapeutic efficacies of C12G6 and other bnAbs in mice

Inflammatory APC show highest expression of TNF superfamily ligands in the lung after LCMV infection. Inflammatory APC show highest expression of TNF superfamily.

Romain Gallet et al. BTS 2016;1:14-28

Volume 19, Issue 3, Pages (March 2011)

Fig. 4. Local application of FR provides prolonged protection against Gq-dependent airway constriction in normal and OVA-sensitized mice in vivo. Local.

Volume 25, Issue 12, Pages e3 (December 2018)

Elevated androgens in global ERα−/− mice decrease lung ILC2 numbers and diminish the KLRG1− ILC2 subset. Elevated androgens in global ERα−/− mice decrease.

Ag 85A–specific immune responses.

Sildenafil suppresses polyp formation in the AOM/DSS model of colon cancer. Sildenafil suppresses polyp formation in the AOM/DSS model of colon cancer.

Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. In utero injection of inflammatory.

Unchanged worm burden and microfilaremia in basophil-deficient mice compared with their basophil-competent littermates. Unchanged worm burden and microfilaremia.

Chimeric HA-based universal influenza virus vaccine concept.

NKTfh cell–driven class switch against polysaccharide Ag is IL-21-influenced. NKTfh cell–driven class switch against polysaccharide Ag is IL-21-influenced.

Volume 9, Issue 6, Pages (June 2011)

Pulmonary vaccination induces a long-term immune memory response to antigen challenge. Pulmonary vaccination induces a long-term immune memory response.

Nrf2−/− mice suffered greater renal damage by STZ compared with Nrf2+/+ mice. Nrf2−/− mice suffered greater renal damage by STZ compared with Nrf2+/+ mice.

Anti-hOX40L treatment prevents aGVHD development in NOG mice transplanted with human PBMCs. NOG mice received human PBMCs (1 × 107) via tail vein. animals.

Schematic representation of the immunization regimens used in this study. Schematic representation of the immunization regimens used in this study. BALB/c.

Slc7a5 deficiency stimulates osteoclastogenesis in vitro.

Prime and boosts with PBK001 vaccine provided 100% protection with low bacterial burden in organs. Prime and boosts with PBK001 vaccine provided 100% protection.

Fig. 1. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy.

Fig. 5 Treatment with BMS (PO BID) protects from wasting and colitis in two SCID mouse models. Treatment with BMS (PO BID) protects from.

Intranasal immunization of mice with S

Functional characterization of multidonor class antibodies.

Fig. 4 In vivo FF results for MMA mice.

Fig. 1. The HCN channel blocker ivabradine (IVA) is analgesic in a mouse model of type 1 diabetes. The HCN channel blocker ivabradine (IVA) is analgesic.

Fig. 6 Multivalent DNA vaccine and protein antigen delivery by T4-AAV nanoparticles. Multivalent DNA vaccine and protein antigen delivery by T4-AAV nanoparticles.

Fig. 4 Alternate-day ocular dosing with DECON curbs HSV-1 in a murine model of ocular infection. Alternate-day ocular dosing with DECON curbs HSV-1 in.

Sang Kyun Ahn, Vanessa Tran, Andrea Leung, Mark Ng, Ming Li, Jun Liu

LAIV and WT influenza virus infection similarly enhance 19F pneumococcal carriage density and duration of colonization. LAIV and WT influenza virus infection.

B- and T-cell activity induced by immunization.

Subodh Verma et al. BTS 2018;3:

In vivo prophylactic and therapeutic efficacy of C12G6 in mice

Expression of 20 genes significantly associated with reduced survivability in GBM is shown across 33 TCGA diseases. Expression of 20 genes significantly.

IL-17A−/− mice are more susceptible to intravaginal HSV-2 challenge following intranasal immunization. IL-17A−/− mice are more susceptible to intravaginal.

The PI3Kβ inhibitor enhances the antitumor activity of T cell–mediated immunotherapy in mice bearing PTEN loss tumors. The PI3Kβ inhibitor enhances the.

Moderate-affinity vaccine antigens elicited greatest antitumor response. Moderate-affinity vaccine antigens elicited greatest antitumor response. Wild-type.

ARQ 531 improves survival in the Eμ-TCL1 engraftment model compared with ibrutinib. ARQ 531 improves survival in the Eμ-TCL1 engraftment model compared.

In the absence of TLR5 and inflammasome recognition, FliC’s D2/D3 domains are essential for secondary IgG1 and IgG2c anti-flagellin responses. In the absence.

Fig. 2. In vivo local CD25-targeted NIR-PIT induces regression of treated LL/2-luc tumors. In vivo local CD25-targeted NIR-PIT induces regression of treated.

Presentation transcript:

Targeted DNA vaccination induced protection against nasal challenge with influenza virus 14 d after DNA vaccination. Targeted DNA vaccination induced protection against nasal challenge with influenza virus 14 d after DNA vaccination. (A) Mice (n = 10 per group) were vaccinated with 25 μg DNA i.m. in each quadriceps and 14 d later challenged with 5xLD50 of influenza virus A (PR8/H1N1). Weight was monitored for 10 d as an indication of disease progression. Weight loss of 20% was defined as humane endpoint at which the mice were euthanized (*p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA). For reasons of clarity only some groups are shown, see (B) for results of all groups. (B) Body weight (%) on day 6 for all the different groups. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the nontargeted αNIP-HA group by Mann–Whitney two-tailed t test. (C) Mean weight on day 6 versus % survival after challenge with influenza A (PR8/H1N1). Correlation analyzed using Pearson correlation two-tailed test. Color coding as in Fig. 3. Ranveig Braathen et al. ImmunoHorizons 2018;2:38-53 Copyright © 2018 The Authors