Evaluation of Nanofluidics Technology for High-Throughput SNP Genotyping in a Clinical Setting Maurice Chan, Mei Wen Chan, Ting Wei Loh, Hai Yang Law,

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Evaluation of Nanofluidics Technology for High-Throughput SNP Genotyping in a Clinical Setting Maurice Chan, Mei Wen Chan, Ting Wei Loh, Hai Yang Law, Chui Sheun Yoon, Sint Sint Than, Jia Mei Chua, Chow Yin Wong, Wei Sean Yong, Yoon Sim Yap, Gay Hui Ho, Peter Ang, Ann Siew Gek Lee The Journal of Molecular Diagnostics Volume 13, Issue 3, Pages 305-312 (May 2011) DOI: 10.1016/j.jmoldx.2010.12.001 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 A: Plot of call rates (average of duplicate wells from two Dynamic Array chips) for 90 samples (from CH-2) against 20 SNP assays. B: Comparison of the allelic discrimination plots between the standard TaqMan Assay and the Dynamic Array for one run of CH-2 samples (47 samples in duplicate). The Journal of Molecular Diagnostics 2011 13, 305-312DOI: (10.1016/j.jmoldx.2010.12.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Plot of call rates for samples from CH-1 (n = 20), CH-2 (n = 47), and KK (n = 47) against 24 SNP assays. The Journal of Molecular Diagnostics 2011 13, 305-312DOI: (10.1016/j.jmoldx.2010.12.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Call map from a 48.48 Dynamic Array using non-column-purified KK samples, column-purified KK samples, and CH-1 samples, run at working concentrations as indicated. The columns correspond to 24 SNP assays loaded in duplicate and the rows correspond to DNA samples (47 samples) and one non-template control (NTC). The red, blue, green, and gray dots correspond to FAM, FAM+VIC (ie, heterozygote), VIC, and no call, respectively. The concentration for sample group D, marked with an asterisk, was derived by dilution from non-column-purified DNA samples at 100 ng/μL. The Journal of Molecular Diagnostics 2011 13, 305-312DOI: (10.1016/j.jmoldx.2010.12.001) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions