Volume 133, Issue 1, Pages 24-33 (July 2007) The Fecal Microbiota of Irritable Bowel Syndrome Patients Differs Significantly From That of Healthy Subjects  Anna Kassinen, Lotta Krogius-Kurikka, Harri Mäkivuokko, Teemu Rinttilä, Lars Paulin, Jukka Corander, Erja Malinen, Juha Apajalahti, Airi Palva  Gastroenterology  Volume 133, Issue 1, Pages 24-33 (July 2007) DOI: 10.1053/j.gastro.2007.04.005 Copyright © 2007 AGA Institute Terms and Conditions

Figure 1 %G+C profiles of intestinal microbial genomic DNA samples from IBS subtypes and control subjects. These included from left to right the fractions 7, 10, and 13, consisting of bacterial genomes with %G+C of 25–30, 40–45, and 55–60, respectively. Gastroenterology 2007 133, 24-33DOI: (10.1053/j.gastro.2007.04.005) Copyright © 2007 AGA Institute Terms and Conditions

Figure 2 Results of the Bayesian population structure analysis on samples from fraction (A) 7, (B) 10, and (C) 13. Each dot represents an identified homogeneous subgroup of bacterial sequences, which is projected into a 2-dimensional space using multidimensional scaling of average relative sequence dissimilarities between the subgroups. In the presence of systematic differences between the subgroup compositions for the controls and the IBS symptom subtypes, the dots appear as widely separated groups. The coloring used here is as follows: control, black; IBS-D, red; IBS-C, green; IBS-M, blue. Gastroenterology 2007 133, 24-33DOI: (10.1053/j.gastro.2007.04.005) Copyright © 2007 AGA Institute Terms and Conditions

Figure 3 qPCR results of fecal samples from 24 patients with IBS and 22 control subjects. For visualization, detection limits of the assays were set to 10416S ribosomal RNA gene copies except for the universal qPCR assay. Symbols are as follows: Control, tick; IBS-C, open circle; IBS-D, cross; IBS-M, triangle. Gastroenterology 2007 133, 24-33DOI: (10.1053/j.gastro.2007.04.005) Copyright © 2007 AGA Institute Terms and Conditions