Isoflurane and sevoflurane increase interleukin-6 levels through the nuclear factor- kappa B pathway in neuroglioma cells L. Zhang, J. Zhang, L. Yang,

Slides:

Advertisements

Similar presentations

Glucocorticoids Augment the Chemically Induced Production and Gene Expression of Interleukin-1α through NF-κB and AP-1 Activation in Murine Epidermal.

Advertisements

EGCG prevents PCB-126-induced endothelial cell inflammation via epigenetic modifications of NF-κB target genes in human endothelial cells Dandan Liu,

Sevoflurane suppresses tumour necrosis factor-α-induced inflammatory responses in small airway epithelial cells after anoxia/reoxygenation K. Watanabe,

High-mobility group box-1 protein induces osteogenic phenotype changes in aortic valve interstitial cells Bo Wang, PhD, Fei Li, MD, Chao Zhang, PhD,

Volume 59, Issue 3, Pages (March 2001)

NF-κB suppresses the expression of ATP-binding cassette transporter A1/G1 by regulating SREBP-2 and miR-33a in mice Guo-Jun Zhao, Shi-Lin Tang, Yun-Cheng.

H. Wang, Y. Dong, J. Zhang, Z. Xu, G. Wang, C. A. Swain, Y. Zhang, Z

Volume 118, Issue 4, Pages (April 2000)

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation Rosa M. Ten, MD, PhDa,

Ketamine and depression

Immunomodulation in the critically ill

Sevoflurane therapy for life-threatening asthma in children

Repression of contexual fear memory induced by isoflurane is accompanied by reduction in histone acetylation and rescued by sodium butyrate T. Zhong,

L-type calcium channels are involved in mediating the anti-inflammatory effects of magnesium sulphate C.Y. Lin, P.S. Tsai, Y.C. Hung, C.J. Huang British.

Suppression of experimental abdominal aortic aneurysms in mice by treatment with pyrrolidine dithiocarbamate, an antioxidant inhibitor of nuclear factor-κB

Potentiation of GABAA receptor activity by volatile anaesthetics is reduced by α5GABAA receptor-preferring inverse agonists I. Lecker, Y. Yin, D.S. Wang,

Characteristics of pain in hospitalized medical patients, surgical patients, and outpatients attending a pain management centre M.P. Rockett, G. Simpson,

J.R. Liu, C. Baek, X.H. Han, P. Shoureshi, S.G. Soriano

Α7 Nicotinic acetylcholine receptor agonist GTS-21 attenuates ventilator-induced tumour necrosis factor-α production and lung injury M. Kox, J.C. Pompe,

Combined treatment with celecoxib and sevoflurane after global cerebral ischaemia has no additive neuroprotective effects in rats J.H. Seo, H.P. Park,

Parkinson's disease and anaesthesia

L. Tong, M. Cai, Y. Huang, H. Zhang, B. Su, Z. Li, H. Dong

Sevoflurane promotes the expansion of glioma stem cells through activation of hypoxia- inducible factors in vitro Q.Y. Shi, S.J. Zhang, L. Liu, Q.S. Chen,

G. Xu, H. Lu, Y. Dong, D. Shapoval, S. G. Soriano, X. Liu, Y. Zhang, Z

Inhibition of mammalian target of rapamycin: Two goals with one shot?

Isoflurane affects the cytoskeleton but not survival, proliferation, or synaptogenic properties of rat astrocytes in vitro D.J. Culley, E.K. Cotran,

Isoflurane enhances the malignant potential of glioblastoma stem cells by promoting their viability, mobility in vitro and migratory capacity in vivo

Daniel R. Meldrum, MD, Brian D

Gemifloxacin inhibits cytokine secretion by lipopolysaccharide stimulated human monocytes at the post-transcriptional level F. Araujo, T. Slifer, S.

Differential effects of serum from patients administered distinct anaesthetic techniques on apoptosis in breast cancer cells in vitro: a pilot study

General anaesthetics do not impair developmental expression of the KCC2 potassium- chloride cotransporter in neonatal rats during the brain growth spurt

Fan Zhang, Yaw L. Siow, Karmin O Kidney International

S. Hagihira British Journal of Anaesthesia

Volume 70, Issue 9, Pages (November 2006)

Acupuncture for treatment of therapy-resistant post-dural puncture headache: a retrospective case series J. Dietzel, T. Witstruck, S. Adler, T.I. Usichenko

Estrogen receptor β agonist diarylpropionitrile inhibits lipopolysaccharide-induced regulated on activation normal T cell expressed and secreted (RANTES)

Inflating LMA with normal saline: 14 yr after

T Emerick, D Metro, R Patel, T Sakai British Journal of Anaesthesia

Differential diagnosis of facial pain and guidelines for management

Editorial I British Journal of Anaesthesia

Expertise in practice: an ethnographic study exploring acquisition and use of knowledge in anaesthesia A. Smith, D. Goodwin, M. Mort, C. Pope British.

Which is most pungent: isoflurane, sevoflurane or desflurane?

Cysteinyl leukotriene upregulates IL-11 expression in allergic airway disease of mice Kyung Sun Lee, PhD, So Ri Kim, MD, PhD, Hee Sun Park, MD, PhD, Seoung.

SIRT1, a Class III Histone Deacetylase, Regulates LPS-Induced Inflammation in Human Keratinocytes and Mediates the Anti-Inflammatory Effects of Hinokitiol

Comparative effects of IL-1β and hydrogen peroxide (H2O2) on catabolic and anabolic gene expression in juvenile bovine chondrocytes G. Martin, Ph.D.,

NF-κB/Rel/IκB: Implications in gastrointestinal diseases

Inflammation-induced up-regulation of ionotropic glutamate receptor expression in human skin P.-H. Tan, L.-C. Yang, P.-T. Chiang, J.S.-C. Jang, H.-C.

C. Price, J. Lee, A.M. Taylor, A.P. Baranowski

Prasun K. Datta, Elias A. Lianos Kidney International

Effects of different remifentanil target concentrations on MAC BAR of sevoflurane in gynaecological patients with CO2 pneumoperitoneum stimulus Z.-Y.

Efficacy of dexmedetomidine compared with midazolam for sedation in adult intensive care patients: a systematic review R. Adams, G.T. Brown, M. Davidson,

Lawrence M. Pfeffer, Andrzej T. Slominski

Aged erythrocytes: a fine wine or sour grapes?

J Lee, S Gupta, C Price, A.P. Baranowski

Chronic widespread pain, including fibromyalgia: a pathway for care developed by the British Pain Society J. Lee, B. Ellis, C. Price, A.P. Baranowski

R Hofmeyr, A Myburgh British Journal of Anaesthesia

A thiol antioxidant regulates IgE isotype switching by inhibiting activation of nuclear factor-κB Yukiyoshi Yanagihara, PhD, Yuji Basaki, MSc, Keiichi.

Impact of anaesthetics and surgery on neurodevelopment: an update

Pharmacological perioperative brain neuroprotection: a qualitative review of randomized clinical trials F. Bilotta, A.W. Gelb, E. Stazi, L. Titi, F.P.

International practices of organ donation

IV. History of anaesthesia in Colombia: periods of development

C.M. Chalmers, A.M. Bal British Journal of Anaesthesia

Influence of perioperative anaesthetic and analgesic interventions on oncological outcomes: a narrative review T. Wall, A. Sherwin, D. Ma, D.J. Buggy

Editorial III British Journal of Anaesthesia

Anti-inflammatory and corticosteroid-enhancing actions of vitamin D in monocytes of patients with steroid-resistant and those with steroid-sensitive asthma

Effect of concentration of local anaesthetic solution on the ED50 of bupivacaine for supraclavicular brachial plexus block† P.K. Gupta, P.M. Hopkins

Anaesthetic drugs and survival: a Bayesian network meta-analysis of randomized trials in cardiac surgery G. Landoni, T. Greco, G. Biondi-Zoccai, C. Nigro.

R. Parke, S. McGuinness, R. Dixon, A. Jull

Toll-like receptors, adapter proteins, and signaling molecules.

Activated protein C inhibits chemotaxis and interleukin-6 release by human neutrophils without affecting other neutrophil functions H.F. Galley, N.E.

Presentation transcript:

Isoflurane and sevoflurane increase interleukin-6 levels through the nuclear factor- kappa B pathway in neuroglioma cells L. Zhang, J. Zhang, L. Yang, Y. Dong, Y. Zhang, Z. Xie British Journal of Anaesthesia Volume 110, Pages i82-i91 (June 2013) DOI: 10.1093/bja/aet115 Copyright © 2013 The Author(s) Terms and Conditions

Fig 1 Isoflurane increases IL-6 in H4 cells. (a) Isoflurane (lanes 4–6) increases IL-6 levels compared with control (lanes 1–3) normalized to β-actin. IL, interleukin. British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions

Fig 2 Isoflurane increases nuclear NF-κB levels and the transcription binding activity of NF-κB. (a) Isoflurane (lanes 4–6) increases NF-κB levels in nuclear extracts from H4 cells relative to control (lanes 1–3). (b) Isoflurane does not change total NF-κB levels in H4 cells. (c) Isoflurane enhances the transcription binding activity of NF-κB in H4 cells. (d) Isoflurane enhances the transcription binding activity of NF-κB in microglia from mice. PDTC attenuates the isoflurane-induced enhancement of the transcription binding activity of NF-κB in mouse microglia. (e) Isoflurane does not enhance the transcription binding activity of NF-κB in primary neurones from mice. NF-κB, nuclear factor-kappa B; LPS, lipopolysaccharide; PDTC, pyrrolidine dithiocarbamate. British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions

Fig 3 PDTC attenuates isoflurane-induced increases in the mRNA and protein levels of IL-6 in H4 cells. (a) Isoflurane increases IL-6 mRNA level in the H4 cells, and PDTC attenuates this increase. (b) Isoflurane increases protein levels of IL-6, and PDTC attenuates this increase. (c) Quantification of the immunoblots shows that PDTC attenuates the isoflurane-induced increase in the protein levels of IL-6. IL, interleukin; PDTC, pyrrolidine dithiocarbamate (n=9). British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions

Fig 4 PDTC attenuates the isoflurane-induced changes in NF-κB in H4 cells. (a) Isoflurane increases nuclear levels of NF-κB, and PDTC attenuates the increase. (b) Quantification of the immunoblots shows that PDTC attenuates the isoflurane-induced increase in nuclear NF-κB levels. (c) PDTC does not alter total NF-κB levels. (d) Quantification of the immunoblots shows that PDTC does not alter total NF-κB levels. (e) PDTC inhibits the isoflurane-induced activation of the transcription binding activity of NF-κB. PTDC, pyrrolidine dithiocarbamate; NF-κB, nuclear factor-kappa B (n=9); LPS, lipopolysaccharide. British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions

Fig 5 Sevoflurane increases IL-6 levels in H4 cells. (a) Sevoflurane treatment (lanes 4–6) increases IL-6 levels. (b) Quantification of the immunoblots shows that sevoflurane increases IL-6 levels. IL, interleukin (n=9). British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions

Fig 6 Sevoflurane increases nuclear NF-κB levels and the transcription binding activity of NF-κB in H4 cells. (a) Sevoflurane treatment (lanes 4–6) increases NF-κB levels in nuclear extracts. (b) Quantification of the immunoblots shows that sevoflurane increases NF-κB levels in nuclear extracts. (c) Sevoflurane does not change total NF-κB levels. (d) Quantification of the immunoblots shows that sevoflurane does not change total NF-κB levels. (e) Sevoflurane enhances the transcription binding activity of NF-κB, and PDTC attenuates the sevoflurane-induced enhancement of the transcription binding activity of NF-κB. NF-κB, nuclear factor-kappa B; LPS, lipopolysaccharide; PDTC, pyrrolidine dithiocarbamate (n=9). British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions

Fig 7 2-DG reduces the isoflurane-induced increase of IL-6 in H4 cells. (a) 2-DG attenuates the isoflurane-induced increase in IL-6 levels. (b) Quantification of the immunoblots shows that 2-DG attenuates the isoflurane-induced increase in IL-6 levels. 2-DG, 2-deoxy-d-glucose; IL, interleukin (n=9). British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions

Fig 8 2-DG reduces the isoflurane-induced increases in nuclear levels of NF-κB in H4 cells. (a) 2-DG inhibits the isoflurane-induced increase in nuclear NF-κB. (b) Quantification of the immunoblots shows that 2-DG inhibits the isoflurane-induced increase in nuclear levels of NF-κB. (c) 2-DG does not alter total NF-κB levels. (d) Quantification of the immunoblots shows that 2-DG treatment does not alter total NF-κB levels. NF-κB, nuclear factor-kappa B; 2-DG, 2-deoxy-d-glucose (n=9). British Journal of Anaesthesia 2013 110, i82-i91DOI: (10.1093/bja/aet115) Copyright © 2013 The Author(s) Terms and Conditions