Bone Marrow Transplant Engraftment Analysis with Loss of an Informative Allele Steven A. Schichman, Pei Lin, Lori J. Gilbrech, Pamela S. Gray, Carla S.

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Presentation transcript:

Bone Marrow Transplant Engraftment Analysis with Loss of an Informative Allele Steven A. Schichman, Pei Lin, Lori J. Gilbrech, Pamela S. Gray, Carla S. Wilson, Jeffrey R. Sawyer The Journal of Molecular Diagnostics Volume 4, Issue 4, Pages 230-232 (November 2002) DOI: 10.1016/S1525-1578(10)60708-8 Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Karyotype of blasts shows t(9;22)(q34;q11) and partial loss of one copy of chromosome 3p at(3)(p21)(arrow). The Journal of Molecular Diagnostics 2002 4, 230-232DOI: (10.1016/S1525-1578(10)60708-8) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 STR genotypes at the D3S1358 and vWA loci for pre- and post-transplant peripheral blood samples. A:Donor. B: Recipient pre-transplant. C: Recipient at 3 months post-transplantation shows genotype identical to the donor indicating 100% donor DNA. D: Recipient at 6 months post-transplantation shows donor and recipient alleles corresponding to a mixture of donor and recipient DNA. Arrow indicates loss of the recipient informative allele D3S1358 (16). Small peak beneath arrow is a stutter peak derived from the donor D3S1358 (17) allele. The Journal of Molecular Diagnostics 2002 4, 230-232DOI: (10.1016/S1525-1578(10)60708-8) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Correspondence of D3S1358 chromosome locus with STR genotype. The D3S1358 locus is located on the short arm of chromosome 3 at (3)(p21). A: Schematic representation of chromosome 3 pair. The short arm and long arm of chromosome 3 are indicated by the letters p and q, respectively. Heterozygous STR genotype is shown beneath the corresponding chromosomes. One copy of chromosome 3 has the D3S1358 (16) allele. One copy of chromosome 3 has the D3S1358 (18) allele. B: Partial deletion of one copy of chromosome 3p at (3)(p21) corresponds to loss of the D3S1358 (16) allele. The Journal of Molecular Diagnostics 2002 4, 230-232DOI: (10.1016/S1525-1578(10)60708-8) Copyright © 2002 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions