Radiation Sources Providing Increased UVA/UVB Ratios Attenuate the Apoptotic Effects of the UVB Waveband UVA-Dose-Dependently in Hairless Mouse Skin

Slides:

Advertisements

Similar presentations

Photobiologic and Photoimmunologic Characteristics of XPA Gene-Deficient Mice Takeshi Horio, Hiroko Miyauchi-Hashimoto, Kazue Kuwamoto, Satoshi Horiki,

Advertisements

Inactivation of the Vitamin D Receptor Enhances Susceptibility of Murine Skin to UV- Induced Tumorigenesis Tara I. Ellison, Molly K. Smith, Anita C. Gilliam,

Direct Detection of Singlet Oxygen Generated by UVA Irradiation in Human Cells and Skin Jürgen Baier, Tim Maisch, Max Maier, Michael Landthaler, Wolfgang.

Systemic Photodynamic Therapy with Aminolevulinic Acid Induces Apoptosis in Lesional T Lymphocytes of Psoriatic Plaques Robert Bissonnette, Dr., Jean-François.

Ultraviolet A (320–400 nm) Modulation of Ultraviolet B (290–320 nm)-Induced Immune Suppression Is Mediated by Carbon Monoxide Munif Allanson, Vivienne.

Topical Treatment with Liposomes Containing T4 Endonuclease V Protects Human Skin In Vivo from Ultraviolet-Induced Upregulation of Interleukin-10 and.

Topical Treatment with Liposomes Containing T4 Endonuclease V Protects Human Skin In Vivo from Ultraviolet-Induced Upregulation of Interleukin-10 and.

De Novo Ceramide Synthesis Participates in the Ultraviolet B Irradiation-Induced Apoptosis in Undifferentiated Cultured Human Keratinocytes Yoshikazu.

Molecular Regulation of UVB-Induced Cutaneous Angiogenesis

[Nle4-D-Phe7]-α-Melanocyte-Stimulating Hormone Significantly Increased Pigmentation and Decreased UV Damage in Fair-Skinned Caucasian Volunteers Ross.

Treatment with 5-Fluorouracil and Celecoxib Displays Synergistic Regression of Ultraviolet Light B-Induced Skin Tumors Traci A. Wilgus, Thomas S. Breza,

Non-Coherent Near Infrared Radiation Protects Normal Human Dermal Fibroblasts from Solar Ultraviolet Toxicity Salatiel Menezes Journal of Investigative.

Delphinidin, an Anthocyanidin in Pigmented Fruits and Vegetables, Protects Human HaCaT Keratinocytes and Mouse Skin Against UVB-Mediated Oxidative Stress.

Reduction of Intrafollicular Apoptosis in Chemotherapy-Induced Alopecia by Topical Calcitriol-Analogs Markus B. Schilli, Ralf Paus Journal of Investigative.

Studies on Epidermis Reconstructed with and without Melanocytes: Melanocytes Prevent Sunburn Cell Formation but not Appearance of DNA Damaged Cells in.

Epidermal COX-2 Induction Following Ultraviolet Irradiation: Suggested Mechanism for the Role of COX-2 Inhibition in Photoprotection Catherine S. Tripp,

Modeling Atopic Dermatitis with Increasingly Complex Mouse Models

Robert S. Stern Journal of Investigative Dermatology

Responses of Black and White Skin to Solar-Simulating Radiation: Differences in DNA Photodamage, Infiltrating Neutrophils, Proteolytic Enzymes Induced,

P38 Mitogen-Activated Protein Kinase Inhibitor Protects the Epidermis Against the Acute Damaging Effects of Ultraviolet Irradiation by Blocking Apoptosis.

UVA/B-Induced Apoptosis in Human Melanocytes Involves Translocation of Cathepsins and Bcl-2 Family Members Cecilia A. Bivik, Petra K. Larsson, Katarina.

Vitamin A Exerts a Photoprotective Action in Skin by Absorbing Ultraviolet B Radiation Christophe Antille, Christian Tran, Olivier Sorg, Pierre Carraux,

Deletion of Prostaglandin E2 EP2 Receptor Protects against Ultraviolet-Induced Carcinogenesis, but Increases Tumor Aggressiveness Sabine Brouxhon, Raymond.

Inhibitory Effect of β-Thujaplicin on Ultraviolet B-Induced Apoptosis in Mouse Keratinocytes Takako Baba, Hajime Nakano, Katsuto Tamai, Daisuke Sawamura,

XPA Gene-Deficient, SCF-Transgenic Mice with Epidermal Melanin Are Resistant to UV- Induced Carcinogenesis Fumikazu Yamazaki, Hiroyuki Okamoto, Hiroko.

Bassel H. Mahmoud, Eduardo Ruvolo, Camile L

Supranuclear Melanin Caps Reduce Ultraviolet Induced DNA Photoproducts in Human Epidermis Nobuhiko Kobayashi, Akemi Nakagawa, Tsutomu Muramatsu, Yukio.

Increased Sensitivity of Histidinemic Mice to UVB Radiation Suggests a Crucial Role of Endogenous Urocanic Acid in Photoprotection Caterina Barresi,

Jean Cadet, Thierry Douki Journal of Investigative Dermatology

Protection against UVR Involves MC1R-Mediated Non-Pigmentary and Pigmentary Mechanisms In Vivo Samantha Robinson, Sandra Dixon, Suzannah August, Brian.

The UV Waveband Dependencies in Mice Differ for the Suppression of Contact Hypersensitivity, Delayed-Type Hypersensitivity and Cis-Urocanic Acid Formation

Differential Regulation of P53 and Bcl-2 Expression by Ultraviolet A and B Yan Wang, Barry Rosenstein, Shira Goldwyn, Xueshu Zhang, Mark Lebwohl, Huachen.

Tej Pratap Singh, Gerlinde Mayer, Peter Wolf

Narrow-Band UVB Induces More Carcinogenic Skin Tumors than Broad-Band UVB through the Formation of Cyclobutane Pyrimidine Dimer Makoto Kunisada, Hiroshi.

Molecular Responses to Photogenotoxic Stress Induced by the Antibiotic Lomefloxacin in Human Skin Cells: From DNA Damage to Apoptosis Laurent Marrot,

Involvement of Photooxidation of Melanogenic Precursors in Prolonged Pigmentation Induced by Ultraviolet A Kazuhisa Maeda, Masato Hatao Journal of Investigative.

Attenuation of UVB-Induced Sunburn Reaction and Oxidative DNA Damage with no Alterations in UVB-Induced Skin Carcinogenesis in Nrf2 Gene-Deficient Mice

Yoshiharu Kawaguchi Journal of Investigative Dermatology

Syed M. Meeran, Thejass Punathil, Santosh K. Katiyar

A Bio-Mimetic Approach to DNA Photoprotection

Society for Investigative Dermatology 2010 Meeting Minutes

Interdependence between Heme Oxygenase-1 Induction and Estrogen-Receptor-β Signaling Mediates Photoimmune Protection by UVA Radiation in Mice Vivienne.

Sitarina Widyarini, Munif Allanson, Nerida L

Research Snippets Journal of Investigative Dermatology

Clinical Snippets Journal of Investigative Dermatology

Journal of Investigative Dermatology

Davina A. Lewis, Dan F. Spandau Journal of Investigative Dermatology

Protective Effect of α-Tocopherol-6-O-Phosphate Against Ultraviolet B-Induced Damage in Cultured Mouse Skin Satomi Nakayama, Shizuko Kobayashi, Ph.D.

Infrared Radiation Confers Resistance to UV-Induced Apoptosis Via Reduction of DNA Damage and Upregulation of Antiapoptotic Proteins Christian Jantschitsch,

Sunlight-Induced Immunosuppression in Humans Is Initially Because of UVB, Then UVA, Followed by Interactive Effects Terence S.C. Poon, Ross St C. Barnetson,

The Repair Enzyme Peptide Methionine-S-Sulfoxide Reductase Is Expressed in Human Epidermis and Upregulated by UVA Radiation Fumihide Ogawa, Christina.

Protective Effect of Hyperpigmented Skin on UV-Mediated Cutaneous Cancer Development Masashi Kato, Nobutaka Ohgami, Yoshiyuki Kawamoto, Toyonori Tsuzuki,

RXRα Ablation in Epidermal Keratinocytes Enhances UVR-Induced DNA Damage, Apoptosis, and Proliferation of Keratinocytes and Melanocytes Zhixing Wang,

Journal of Investigative Dermatology

Immunoprotective UVA (320–400 nm) Irradiation Upregulates Heme Oxygenase-1 in the Dermis and Epidermis of Hairless Mouse Skin Munif Allanson, Vivienne.

UV Light from 290 to 325 nm, but not Broad-Band UVA or Visible Light, Augments the Formation of Melanocytic Nevi in a Guinea-Pig Model for Human Nevi

An Important Role of Lymphatic Vessels in the Control of UVB-Induced Edema Formation and Inflammation Kentaro Kajiya, Michael Detmar Journal of Investigative.

1,25(OH)2D3 and JN reduce UVR-induced CPD, SBCs, and immunosuppression in Skh:hr1 mouse skin. 1,25(OH)2D3 and JN reduce UVR-induced CPD, SBCs, and immunosuppression.

Ultraviolet A Irradiation of C57BL/6 Mice Suppresses Systemic Contact Hypersensitivity or Enhances Secondary Immunity Depending on Dose Scott N. Byrne,

Protection Against Pyrimidine Dimers, p53, and 8-hydroxy-2′-Deoxyguanosine Expression in Ultraviolet-Irradiated Human Skin by Sunscreens: Difference Between.

Evaluation of Apoptotic Cells Induced by Ultraviolet Light B Radiation in Epidermal Sheets Stained by the TUNEL Technique Hiroyuki Okamoto, Kana Mizuno,

Galectin-3 Protects Keratinocytes from UVB-Induced Apoptosis by Enhancing AKT Activation and Suppressing ERK Activation Jun Saegusa, Daniel K. Hsu, Wei.

Induction of Interleukin-6 Production by Ultraviolet Radiation in Normal Human Epidermal Keratinocytes and in a Human Keratinocyte Cell Line is Mediated.

The Spectrum of Mitochondrial DNA Deletions is a Ubiquitous Marker of Ultraviolet Radiation Exposure in Human Skin Amanda J. Ray, Richard Turner, Osamu.

Journal of Investigative Dermatology

Ultraviolet B Radiation Upregulates the Production of Macrophage Migration Inhibitory Factor (MIF) in Human Epidermal Keratinocytes Tadamichi Shimizu,

Photoimmunoprotection by UVA (320–400nm) Radiation Is Determined by UVA Dose and Is Associated with Cutaneous Cyclic Guanosine Monophosphate Munif Allanson,

UVA Radiation Induces Suppression of Perforin-Granule Release from CD8hi+ Cytotoxic T Lymphocytes Ex Vivo Andreas Ambach, Bernd Bonnekoh, Harald Gollnick

Modulation of IL-10, IL-12, and IFN-γ in the Epidermis of Hairless Mice by UVA (320– 400 nm) and UVB (280–320 nm) Radiation Jie Shen, Shisan Bao, Vivienne.

Ulrike P. Kappes, Dan Luo, Marisa Potter, Karl Schulmeister, Thomas M

Presentation transcript:

Radiation Sources Providing Increased UVA/UVB Ratios Attenuate the Apoptotic Effects of the UVB Waveband UVA-Dose-Dependently in Hairless Mouse Skin Yuko Ibuki, Munif Allanson, Katie M. Dixon, Vivienne E. Reeve Journal of Investigative Dermatology Volume 127, Issue 9, Pages 2236-2244 (September 2007) DOI: 10.1038/sj.jid.5700856 Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Formation of sunburn cells after exposure to SSUV. (a) Light microscopy of histochemical identification (H&E stain) of sunburn cells. Vertical paraffin sections of normal (Nil) or irradiated skin at 24hours post-exposure to SSUV (240V). Sunburn cells are shown by arrows. (b) Average number of sunburn cells/linear cm of skin at 0, 24, and 72hours post-exposure to SSUV (240V) and UVA-enhanced radiation (UVB at 180V). Error bars=SD. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Increased UVA/UVB ratio reduces sunburn cell formation. Average number of sunburn cells/cm at 24hours after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. Symbols indicate statistically significantly different treatments. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Increased UVA/UVB ratio decreases epidermal DNA fragmentation. Appearance of DNA fragmentation after exposure to SSUV (240V) and decreased fragmentation with increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Formation of caspase-3-positive cells after exposure to UV. (a) Light microscopy of immunohistochemical identification of caspase-3 positivity. Vertical paraffin sections of normal (Nil) or irradiated skin at 24hours post-exposure to 3MEdD of SSUV (240V). Positive cells are stained brown in the epidermal layer. (b) Average number of caspase-3-positive cells/linear cm of skin after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. Symbol indicates statistically significantly different treatments. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Effect of attenuation of SSUV fluence rate on sunburn cell formation. Average number of sunburn cells/linear cm of skin at 24hours post-exposure to 3MEdD of SSUV delivered without spectral change at 240, 180, or 160V. Error bars=SD. No significant difference between treatments. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 6 Effect of attenuation of SSUV fluence rate on caspase-3-positive cell formation. Average number of caspase-3-positive cells/linear cm of skin at 24hours after exposure to 3MEdD of SSUV delivered without spectral change at 240, 180, or 160V. Error bars=SD. No significant difference between treatments. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 7 Reduced UVB fluence rate has no effect on epidermal thymine dimer formation. Effect of attenuation of the fluence rate of UVB irradiation delivered alone on formation of epidermal thymine dimers at 24hours post-exposure to 3MEdD of UVB. Immunohistochemical detection of DNA dimers in groups of three mice, with UVB voltage reduction from 240 to 180 and 160V, compared with unirradiated skin (Nil), and semiquantitation of average positive nuclei using image analysis. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 8 Inhibition of heme oxygenase activity abrogates UVA protection against sunburn cell formation. Effect of SnPP treatment on average number of sunburn cells/linear cm of skin at 24hours after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. No significant difference between treatments. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 9 Inhibition of heme oxygenase activity decreases UVA protection against caspase-3 positive cell formation. Effect of SnPP treatment on average number of caspase-positive cells/linear cm of skin at 24hours after exposure to increased ratios of UVA/UVB (as UVB voltage was reduced from 240 to 180 and 160V). Error bars=SD. Journal of Investigative Dermatology 2007 127, 2236-2244DOI: (10.1038/sj.jid.5700856) Copyright © 2007 The Society for Investigative Dermatology, Inc Terms and Conditions