UVA and UVB Induce Different Sets of Long Noncoding RNAs Kazuyuki Yo, Thomas M. Rünger  Journal of Investigative Dermatology  Volume 137, Issue 3, Pages 769-772 (March 2017) DOI: 10.1016/j.jid.2016.10.041 Copyright © 2016 The Authors Terms and Conditions

Figure 1 UVA and UVB induce different sets of long noncoding RNAs. Microarray profiles of lncRNA expression in primary human neonatal fibroblasts irradiated with UVA (200 kJ/m2; 100 kJ/m2 with repeated exposures on three consecutive days) or UVB (500 J/m2; 200 J/m2 for repeated exposures on three consecutive days) were determined using Human LncRNA Array v3.0. (a) Heat maps were drawn for lncRNAs whose expression was up- or downregulated more than twofold with a P value < 0.05: The right three columns of each panel show relative expression (z-score) of unirradiated control fibroblasts, and the left two columns the relative expression of irradiated fibroblasts. Because the functions of most lncRNAs (>99%) are not known, heat maps of lncRNAs were organized according to the similarity of their expression pattern. (b) Venn diagrams demonstrating the number of lncRNAs affected by both UVA and UVB (upper two diagrams), by the three different irradiation conditions with UVA (middle two diagrams), or UVB (lower two diagrams). The overlaps of lncRNA genes upregulated by the different conditions are shown on the left, and those of downregulated ones on the right. lncRNA, long noncoding RNA. Journal of Investigative Dermatology 2017 137, 769-772DOI: (10.1016/j.jid.2016.10.041) Copyright © 2016 The Authors Terms and Conditions

Figure 2 Changes in the expression of UVA- or UVB-altered long noncoding RNAs, as confirmed by qPCR and FISH. The change in the expression of the lncRNAs GS1-600G8.5 (a), RP13-631K18.2 (b), LOC389641 (c), and IMMP2L-IT1 (d), as shown by microarray (left column) and qPCR (right column) in primary human neonatal fibroblasts. The microarray data show means of duplicate irradiated samples and of triplicate, unirradiated controls. The qPCR data show results of triplicate controls and irradiated samples, mean ± SD (*P < 0.05, **P < 0.01 compared with sham controls). (e) Representative examples of confocal microscopy of fibroblasts stained with FITC-labeled probes for LOC389641, scale bar = 20 μm. FISH, fluorescence in situ hybridization; lncRNA, long noncoding RNA; qPCR, quantitative real-time polymerase chain reaction. Journal of Investigative Dermatology 2017 137, 769-772DOI: (10.1016/j.jid.2016.10.041) Copyright © 2016 The Authors Terms and Conditions