ECM 29 and Kaplan–Meier survival curves for additional datasets

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ECM 29 and Kaplan–Meier survival curves for additional datasets ECM 29 and Kaplan–Meier survival curves for additional datasets Proteasome activity levels, as measured by Me4BodipyFL‐Ahx3Leu3VS probe, after siRNA‐mediated knockdown of ECM29 and subsequent 6‐h treatment with palbociclib (1 μM) or Torin‐1 (0.1 μM) in HeLa cells (n = 4).MCF7 (left) and T47D (right) proliferation after ECM29 or control siRNAs transfection [n = 4 (MCF7) or 3 (T47D)]. Western blots display the knockdown efficiency after 48 h.ECM29 expression in GSE37751 dataset (Terunuma et al, 2014) (left) and ECM29 expression in TCGA breast cancer data. The survival data are from PROGgeneV2—Pan Cancer Prognostics Database (http://watson.compbio.iupui.edu/chirayu/proggene/database/index.php). Statistical analysis of the Kaplan–Meier (KM) plots has been described (Goswami & Nakshatri, 2014).Data information: In panels (A and B), data are presented as means ± SD; each n represents an individual biological replicate; P‐values were determined by ANOVA and two‐tailed Student's t‐test with Holm–Sidak post hoc test. Teemu P Miettinen et al. EMBO J. 2018;embj.201798359 © as stated in the article, figure or figure legend