Detection of cross-transmission of multiresistant Gram-negative bacilli and Staphylococcus aureus in adult intensive care units by routine typing of clinical.

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Presentation transcript:

Detection of cross-transmission of multiresistant Gram-negative bacilli and Staphylococcus aureus in adult intensive care units by routine typing of clinical isolates Hajo Grundmann, Axel Hahn, Boris Ehrenstein, Klaus Geiger, Hansjörg Just, Franz D. Daschner Clinical Microbiology and Infection Volume 5, Issue 6, Pages 355-363 (June 1999) DOI: 10.1111/j.1469-0691.1999.tb00154.x Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 Dendrogram for 48 isolates of Pseudomonas aeruginosa obtained from patients in two ICUs over a 12-month investigation period, including replicate analysis of a ‘repro’ strain. Illustrated are the degree of correlation between isolates from different patients (r × 100), the sample number, type of ICU, and date of the first and last isolation of possible cross-transmitted pathogens. Clinical Microbiology and Infection 1999 5, 355-363DOI: (10.1111/j.1469-0691.1999.tb00154.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 Representative fluorescence densitogram of MR GNB from clinical specimens isolated from patients during a 3-week interval (21 July to 12 August 1994) in two ICUs. Lanes 2–6, isolates from MICU; Lanes 7–13, isolates from SICU. Lane 1: 100-bp-ladder size marker. Lane 2: Serratia marcescens from tracheal secretion of patient F.R., isolated on 24 July. Lane 3: Serratia marcescens from tracheal secretion of patient B.H., isolated on 1 August, same clone as lane 2. Lane 4: Pseudomonas aeruginosa cultured from the same tracheal secretion as Serratia marcescens (patient F.R.) in lane 2. Lane 5: Pseudomonas aeruginosa from tracheal secretion of patient U.W., isolated on 7 August, same clone as in lane 4. Lane 6: Pseudomonas aeruginosa from tracheal secretion of patient S.B., isolated on 6 August, unrelated to the transmission clone. Lanes 7 and 8: two isolates of Pseudomonas aeruginosa which differed by colony morphology, from the same tracheal secretion of patient M.E., isolated on 5 August. Lane 9: Pseudomonas aeruginosa from wound swab of patient A.H., isolated on 4 August, same clone as lanes 7 and 8. Lane 10: Pseudomonas aeruginosa from tracheal secretion of patient K.V., isolated on 12 August, unrelated to transmission clone. Lane 11: A. calcoaceticus/baumannii from tracheal secretion of patient B.J., isolated on 21 July. Lane 12: A. calcoaceticus/baumannii from tracheal secretion of patient S.A., isolated on 27 July, same clone as lane 11. Lane 13: A. calcoaceticus/baumannii cultured from the same tracheal secretion as Pseudomonas aeruginosa (patient M.E.) in lanes 7 and 8 but bearing no relation to the transmission clone in lanes 11 and 12. All isolates had been produced in parallel and PCR products were run in the same gel. Lanes with repro strains and other size marker lanes have been omitted. Clinical Microbiology and Infection 1999 5, 355-363DOI: (10.1111/j.1469-0691.1999.tb00154.x) Copyright © 1999 European Society of Clinical Infectious Diseases Terms and Conditions