Rapid detection of enterobacteriaceae producing extended spectrum beta-lactamases directly from positive blood cultures by matrix-assisted laser desorption.

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Presentation transcript:

Rapid detection of enterobacteriaceae producing extended spectrum beta-lactamases directly from positive blood cultures by matrix-assisted laser desorption ionization-time of flight mass spectrometry M. Oviaño, B. Fernández, A. Fernández, M.J. Barba, C. Mouriño, G. Bou Clinical Microbiology and Infection Volume 20, Issue 11, Pages 1146-1157 (November 2014) DOI: 10.1111/1469-0691.12729 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 (a) Mass spectra of cefotaxime at 60 min (A), 90 min (B), 120 min (C) and 180 min (D) and ceftazidime at 60 min (E), 90 min (F), 120 min (G) and 180 min (H) after exposure to the bacterial lysate of one isogenic Escherichia coli control strain expressing blaCTX-M-32. (1b) Mass spectra of cefotaxime at 60 min (A), 90 min (B), 120 min (C) and 180 min (D) and ceftazidime at 60 min (E), 90 min (F), 120 min (G) and 180 min (H) after exposure to the bacterial lysate of one isogenic E. coli control strain expressing blaCMY-2. a Indicates the hydrolized peaks of the antibiotic. b→Indicates the antibiotic mass peaks (no hydrolyzed form). Clinical Microbiology and Infection 2014 20, 1146-1157DOI: (10.1111/1469-0691.12729) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 (a) Mass spectra of cefotaxime at 60 min (A), 90 min (B), 120 min (C) and 180 min (D) and ceftazidime at 60 min (E), 90 min (F), 120 min (G) and 180 min (H) after exposure to the bacterial lysate of one isogenic Escherichia coli control strain expressing blaCTX-M-32. (1b) Mass spectra of cefotaxime at 60 min (A), 90 min (B), 120 min (C) and 180 min (D) and ceftazidime at 60 min (E), 90 min (F), 120 min (G) and 180 min (H) after exposure to the bacterial lysate of one isogenic E. coli control strain expressing blaCMY-2. a Indicates the hydrolized peaks of the antibiotic. b→Indicates the antibiotic mass peaks (no hydrolyzed form). Clinical Microbiology and Infection 2014 20, 1146-1157DOI: (10.1111/1469-0691.12729) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 (a) Mass spectra of cefotaxime (A), cefotaxime plus clavulanic acid (B), ceftazidime (C) and ceftazidime plus clavulanic acid (D) after exposure to the bacterial lysate at the time when the hydrolysis is higher. β-lactamase-type and time studied is indicated in the top right corner. β-lactamases belong to control strains are as follows: TEM-1 (non-ESBL), SHV-12 (ESBL), TEM-29 (ESBL with higher activity toward ceftazidime), CTX-M-14, -15, and -32 (ESBL), FOX-4, CMY-2, DHA-1, -6 and -7 (plasmidic AmpC-type enzymes) a Indicates the hydrolized peaks of the antibiotic. b→Indicates the antibiotic mass peaks (no hydrolyzed form). Clinical Microbiology and Infection 2014 20, 1146-1157DOI: (10.1111/1469-0691.12729) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 (a) Mass spectra of cefotaxime (A), cefotaxime plus clavulanic acid (B), ceftazidime (C) and ceftazidime plus clavulanic acid (D) after exposure to the bacterial lysate at the time when the hydrolysis is higher. β-lactamase-type and time studied is indicated in the top right corner. β-lactamases belong to control strains are as follows: TEM-1 (non-ESBL), SHV-12 (ESBL), TEM-29 (ESBL with higher activity toward ceftazidime), CTX-M-14, -15, and -32 (ESBL), FOX-4, CMY-2, DHA-1, -6 and -7 (plasmidic AmpC-type enzymes) a Indicates the hydrolized peaks of the antibiotic. b→Indicates the antibiotic mass peaks (no hydrolyzed form). Clinical Microbiology and Infection 2014 20, 1146-1157DOI: (10.1111/1469-0691.12729) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 (a) Mass spectra of cefotaxime (A), cefotaxime plus clavulanic acid (B), ceftazidime (C) and ceftazidime plus clavulanic acid (D) after exposure to the bacterial lysate at the time when the hydrolysis is higher. β-lactamase-type and time studied is indicated in the top right corner. β-lactamases belong to control strains are as follows: TEM-1 (non-ESBL), SHV-12 (ESBL), TEM-29 (ESBL with higher activity toward ceftazidime), CTX-M-14, -15, and -32 (ESBL), FOX-4, CMY-2, DHA-1, -6 and -7 (plasmidic AmpC-type enzymes) a Indicates the hydrolized peaks of the antibiotic. b→Indicates the antibiotic mass peaks (no hydrolyzed form). Clinical Microbiology and Infection 2014 20, 1146-1157DOI: (10.1111/1469-0691.12729) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 (a) Mass spectra of cefotaxime (A), cefotaxime plus clavulanic acid (B), ceftazidime (C) and ceftazidime plus clavulanic acid (D) after exposure to the bacterial lysate at the time when the hydrolysis is higher. β-lactamase-type and time studied is indicated in the top right corner. β-lactamases belong to control strains are as follows: TEM-1 (non-ESBL), SHV-12 (ESBL), TEM-29 (ESBL with higher activity toward ceftazidime), CTX-M-14, -15, and -32 (ESBL), FOX-4, CMY-2, DHA-1, -6 and -7 (plasmidic AmpC-type enzymes) a Indicates the hydrolized peaks of the antibiotic. b→Indicates the antibiotic mass peaks (no hydrolyzed form). Clinical Microbiology and Infection 2014 20, 1146-1157DOI: (10.1111/1469-0691.12729) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions