European Urology Oncology Individual Comparison of Cholesterol Metabolism in Normal and Tumour Areas in Radical Prostatectomy Specimens from Patients with Prostate Cancer: Results of the CHOMECAP Study  Olivier Celhay, Laura Bousset, Laurent Guy, Jean-Louis Kemeny, Valerio Leoni, Claudio Caccia, Amalia Trousson, Christelle Damon- Soubeyrant, Angélique De Haze, Laura Sabourin, Catherine Godfraind, Cyrille de Joussineau, Bruno Pereira, Laurent Morel, Jean Marc Lobaccaro, Silvère Baron  European Urology Oncology  DOI: 10.1016/j.euo.2018.08.001 Copyright © 2018 Terms and Conditions

Fig. 1 After radical prostatectomy, samples of normal and tumour tissues were collected from each organ and frozen. Prostate specimens were retrospectively fixed, paraffin-embedded, and stained with haematoxylin and eosin. Three examples are shown. Normal tissue (blue) and carcinoma (red) areas in samples were confirmed by a pathologist to validate the sample collection. Tissues were then processed for gene analysis, sterol measurement, and immunohistochemistry. European Urology Oncology DOI: (10.1016/j.euo.2018.08.001) Copyright © 2018 Terms and Conditions

Fig. 2 Graphical summary of individual comparisons of tumour and normal tissues. The cholesterol pathway is represented by three boxes: the mevalonate pathway, cholesterol homeostasis, and cholesterol oxidation. Gene expression and metabolites showing significant differences are indicated in green for downregulation and red for upregulation. The magnitude of changes is indicated as a percentage. European Urology Oncology DOI: (10.1016/j.euo.2018.08.001) Copyright © 2018 Terms and Conditions

Fig. 3 Haematoxylin and eosin (left) and SCARB1 immunostaining (right) for two examples of normal and tumour prostate tissues pairs. The boxplot shows the H-scores for SCARB1 staining in both tissues. ** p≤0.01. The histogram depicts the difference in SCARB1 H-score between tumour and normal tissues for each individual patient. The red line indicates the median H-score. European Urology Oncology DOI: (10.1016/j.euo.2018.08.001) Copyright © 2018 Terms and Conditions

Fig. 4 Principal component analysis for gene expression results. Global vectors are shown for tumour and normal tissues using red and blue areas, respectively, and the heading for each vector denotes genes with the most deregulated expression in the tissue pairs. Deep blue/red circles indicate genes involved in de novo cholesterol synthesis that form an aggregated cluster. European Urology Oncology DOI: (10.1016/j.euo.2018.08.001) Copyright © 2018 Terms and Conditions