Enhanced In Vivo Function of Bioartificial Lungs in Rats

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Presentation transcript:

Enhanced In Vivo Function of Bioartificial Lungs in Rats Jeremy J. Song, BS, Sam S. Kim, MD, Zhilin Liu, PhD, Joren C. Madsen, MD, DPhil, Douglas J. Mathisen, MD, Joseph P. Vacanti, MD, Harald C. Ott, MD The Annals of Thoracic Surgery Volume 92, Issue 3, Pages 998-1006 (September 2011) DOI: 10.1016/j.athoracsur.2011.05.018 Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Function of cadaveric and bioartificial lung transplant recipients in vivo. (A) Partial arterial oxygen pressure (PaO2) in femoral artery blood samples in pneumonectomized controls (black), and cadaveric (dark gray), and bioartificial (light gray) lung transplant recipients on postoperative (po) day (POD) 1, 7, and 14 at 100% fraction of inspired oxygen (FiO2). (B) Corresponding PaO2 values at 21% FiO2. (C) Dynamic compliance measured in vivo at a peak inspiratory pressure of 25 cmH2O in controls (black), and cadaveric (dark gray), and bioartificial (light gray) lung transplant recipients on POD 1, 7, and 14. (D) The PaO2 values of cadaveric (dark gray) and bioartificial (dark gray) lung transplant recipients on POD 14 after clamping of the right pulmonary artery at 100% FiO2. (RA = room air; TX = transplantation.) The Annals of Thoracic Surgery 2011 92, 998-1006DOI: (10.1016/j.athoracsur.2011.05.018) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Bioartificial lung explants after transplantation. (A) Isolated heart and lung en bloc resection of a bioartificial lung transplant recipient, ventilated through trachea, on inflation (left) and deflation (right) 24 hours after transplantation. Black arrow marks the transplanted bioartificial left lung. (B) Isolated heart and lung en bloc resection 14 days after transplantation, inflated (left), and deflated (right), black arrowhead marking the transplanted bioartificial left lung. The Annals of Thoracic Surgery 2011 92, 998-1006DOI: (10.1016/j.athoracsur.2011.05.018) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Fluoroscopy images of transplant recipients. Representative fluoroscopy images of cadaveric transplant recipients (upper panel) and bioartificial lung recipients (lower panel) 24 hours, 7 days, and 14 days after lung transplantation (LTX) on maximal spontaneous inspiration. Bioartificial lung recipients show a gradual increase in radiographic density of the transplanted lungs compared with cadaveric transplant recipients. (POD = postoperative day.) The Annals of Thoracic Surgery 2011 92, 998-1006DOI: (10.1016/j.athoracsur.2011.05.018) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Histology of bioartificial lungs before and after transplantation. (A) Immunohistochemical stains of bioartificial lung tissue before transplantation confirming engraftment of type II pneumocytes (thyroid transcription factor [TTF-1], prosurfactant protein C [pro-SPC]), Clara cells (CC-10), and mesenchymal cells (Vimentin). Black arrowheads mark a zone of transition from cuboidal distal airway epithelium to an area of larger diameter and narrower epithelium similar to a bronchoalveolar duct junction. (B) Hematoxylin-eosin stain and immunohistochemical stains of bioartificial lung tissue 24 hours after transplantation (left). Immunohistochemical stains for pro-SPC, Vimentin, and ED-1 (CD 68) (right). Black arrowheads mark an alveolar duct. (C) Hematoxylin-eosin stain (left) and immunohistochemical stains for pro-SPC, Vimentin, and ED-1 of bioartificial lung tissue 14 days after transplantation (right). Black arrowheads mark a zone of increased density and cellularity similar to a fibrous pleural scar. (D) TTF-1, pro-SPC, CC-10, and Vimentin stains of native rat lung served as positive controls. (Original magnification: A and D, 40X; B and C, 20X.) The Annals of Thoracic Surgery 2011 92, 998-1006DOI: (10.1016/j.athoracsur.2011.05.018) Copyright © 2011 The Society of Thoracic Surgeons Terms and Conditions