Volume 72, Issue 7, Pages 853-864 (October 2007) Leukocyte gene expression signatures in antineutrophil cytoplasmic autoantibody and lupus glomerulonephritis  D.A. Alcorta, D.A. Barnes, M.A. Dooley, P. Sullivan, B. Jonas, Y. Liu, S. Lionaki, C.B. Reddy, H. Chin, A.A. Dempsey, J.C. Jennette, R.J. Falk  Kidney International  Volume 72, Issue 7, Pages 853-864 (October 2007) DOI: 10.1038/sj.ki.5002371 Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 1 Hierarchical clusters of ANOVA-selected differentially expressed genes in SLE, ANCA, RA, and healthy volunteer leukocyte samples. The Eisen clustering data are visualized with TreeView and shown as a heat map. Graphical representation of gene expression differences are shown with green corresponding to down-regulated and red with up-regulated expression with the maximum intensity corresponding to a change of 0.16 based upon log10. Individual patient samples span the horizontal axis while individual genes are divided along the vertical axis. SLE, ANCA, and healthy donor gene signatures are labeled. At the top of the figure is the dendrogram showing the relationship of the donor samples to one another. The white boxes contain genes that enhance differentiation of SLE and ANCA gene signatures. Kidney International 2007 72, 853-864DOI: (10.1038/sj.ki.5002371) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 2 Expression analysis of the ANCA BVAS genes in in vitro stimulated healthy donor leukocyte cell subtypes (see Table 6). (a) Expression levels of these genes (Table 4) were determined in stimulated monocytes, T cells, B cells, neutrophils and Natural Killer cells from healthy donors. (b and c) Predominant sample clusters of neutrophils and T lymphocytes are boxed and are shown. Kidney International 2007 72, 853-864DOI: (10.1038/sj.ki.5002371) Copyright © 2007 International Society of Nephrology Terms and Conditions

Figure 3 Expression analysis of the SLE signature genes in in vitro stimulated healthy donor leukocyte cell subtypes (see Table 6). (a) Dendrogram of expression levels of these genes as determined in stimulated monocytes, T cells, B cells, and neutrophils from healthy donors. The predominant sample cluster of activated monocytes and T lymphocytes is boxed and the cells and stimuli used are shown in b. Kidney International 2007 72, 853-864DOI: (10.1038/sj.ki.5002371) Copyright © 2007 International Society of Nephrology Terms and Conditions