Analysis of WHITE COLLAR 2 isoforms.

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Analysis of WHITE COLLAR 2 isoforms. Analysis of WHITE COLLAR 2 isoforms. (A) Expression of full‐length, medium (asterisk) and short (s) WC2 isoforms in wild type (wt), Δfrq and Δwc1 at DD24 (constant darkness for 24h). (B) WC2 isoforms in LL in Δwc2 expressing WC2 with a carboxy‐terminal DHFR tag under the control of the wc2 promoter P 2.2. Tagged and non‐tagged WC2 isoforms are schematically outlined. Grey box: DHFR (D). (C) WC2 isoforms in Δwc2 expressing WC2 with an amino‐terminal TAP tag under the control of the inducible qa2 promoter in the presence (+) or absence (−) of quinic acid (QA). Cultures were grown in LL. Tagged and non‐tagged WC2 isoforms are schematically outlined. Black box: TAP tag (T). (D) Transcription start sites of Pint‐wc2 identified by 5′ RNA ligase‐mediated rapid amplification of complementary DNA ends at DD16. Distances from +1 are indicated. Grey bars: putative ATG start codons for sWC2. (E) Expression of WC2 isoforms in LL in Δwc2 transformed with P 0.6 and P 1.2–0.6. (F) Ratio of wc2 RNA in Δwc1 versus Δfrq measured by quantitative real‐time reverse transcription–PCR at DD24 using probes specific to exon 1 (E1) and exon 2 (E2). DD, constant darkness; FRQ, FREQUENCY; LL, constant light; WC, WHITE COLLAR. Andrea Neiss et al. EMBO Rep. 2008;9:788-794 © as stated in the article, figure or figure legend