Suppression of antigen-specific T- and B-cell responses by intranasal or oral administration of recombinant Bet v 1, the major birch pollen allergen,

Slides:

Advertisements

Similar presentations

Yvan Boutin, PhD, Jacques Hébert, MD

Advertisements

Immunomodulatory effects of aqueous birch pollen extracts and phytoprostanes on primary immune responses in vivo Jan Gutermuth, MD, Mayte Bewersdorff,

Heat-killed Lactobacillus plantarum L-137 suppresses naturally fed antigen–specific IgE production by stimulation of IL-12 production in mice Shinji.

CD4 T-helper cells engineered to produce IL-10 prevent allergen-induced airway hyperreactivity and inflammation Jae-Won Oh, MD, PhD, Christine M. Seroogy,

Designing hypoallergenic derivatives for allergy treatment by means of in silico mutation and screening Theresa Thalhamer, PhD, Heidi Dobias, MSc, Tatjana.

Identification of allergens in fruits and vegetables: IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin)

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma Tobias Polte, PhD, Christian.

Frank Kirstein, PhD, Natalie E

Induction of IgE antibodies with predefined specificity in rhesus monkeys with recombinant birch pollen allergens, Bet v 1 and Bet v 2 Fatima D. Ferreira,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

Group 2 innate lymphoid cells facilitate sensitization to local, but not systemic, TH2- inducing allergen exposures Matthew J. Gold, BSc, Frann Antignano,

Allergen-specific IgE production of committed B cells from allergic patients in vitro Peter Steinberger, MSca, Barbara Bohlea, Franco di Padova, MDb,

Rush immunotherapy results in allergen-specific alterations in lymphocyte function and interferon-γ production in CD4+ T cells Gideon Lack, MD, Harold.

Grass pollen immunotherapy inhibits allergen-induced infiltration of CD4+ T lymphocytes and eosinophils in the nasal mucosa and increases the number of.

Notch signaling in T cells is essential for allergic airway inflammation, but expression of the Notch ligands Jagged 1 and Jagged 2 on dendritic cells.

Histamine in the immune regulation of allergic inflammation

Forkhead box protein 3 demethylation is associated with tolerance induction in peanut- induced intestinal allergy Meiqin Wang, MD, PhD, Ivana V. Yang,

Induction of IgE antibodies in mice and rhesus monkeys with recombinant birch pollen allergens: Different allergenicity of Bet v 1 and Bet v 2 Susanne.

Frank Kirstein, PhD, Natalie E

Update on Immune Mechanisms Associated with Sublingual Immunotherapy: Practical Implications for the Clinician Philippe Moingeon, PhD The Journal of.

Specific epicutaneous immunotherapy prevents sensitization to new allergens in a murine model Lucie Mondoulet, PhD, Vincent Dioszeghy, PhD, Emilie Puteaux,

Persistent protective effect of heat-killed Escherichia coli producing “engineered,” recombinant peanut proteins in a murine model of peanut allergy

Culture medium from TNF-α–stimulated mesenchymal stem cells attenuates allergic conjunctivitis through multiple antiallergic mechanisms Wenru Su, MD,

Ellen Mueller Fox, PhD, Marina N

Severe oral allergy syndrome and anaphylactic reactions caused by a Bet v 1– related PR-10 protein in soybean, SAM22 Jörg Kleine-Tebbe, MDa, Andrea Wangorsch,

Mucosal Immunology of Food Allergy

Efficacy of local nasal immunotherapy for Dp2-induced airway inflammation in mice: Using Dp2 peptide and fungal immunomodulatory peptide Yi-Hsia Liu,

Takao Kobayashi, PhD, Koji Iijima, PhD, Alexander L

A fusion protein of flagellin and ovalbumin suppresses the TH2 response and prevents murine intestinal allergy Stefan Schülke, PhD, Manja Burggraf, MSc,

Serum IgE response to orally ingested antigen: A novel IgE response model with allergen-specific T-cell receptor transgenic mice Kan Shida a,b, Satoshi.

Iván López-Expósito, PhD, Ying Song, MD, Kirsi M

Peanut-induced intestinal allergy is mediated through a mast cell–IgE–FcεRI–IL-13 pathway Meiqin Wang, MD, PhD, Katsuyuki Takeda, MD, PhD, Yoshiki Shiraishi,

Yanli Ouyang, MD, PhDa, Nisha Virasch, BSa, Ping Hao, MDa, Michael T

Orally administered TGF-β is biologically active in the intestinal mucosa and enhances oral tolerance Takashi Ando, MD, PhD, Kyosuke Hatsushika, MD,

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Mukesh Kumar, PhDa, Aruna K. Behera, PhDa, Jianan Hu, MDa, Richard F

Ana Belén Blázquez, Lloyd Mayer, M. Cecilia Berin Gastroenterology

Kirthana Ganeshan, BS, Colleen V

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

IFN-τ inhibits IgE production in a murine model of allergy and in an IgE-producing human myeloma cell line Mustafa G. Mujtaba, PhDa, Lorelie Villarete,

Protease-dependent activation of epithelial cells by fungal allergens leads to morphologic changes and cytokine production Henk F. Kauffman, PhD, J.F.Chris.

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Increased expression of the CD80 accessory molecule by alveolar macrophages in asthmatic subjects and its functional involvement in allergen presentation.

Allergen-specific sublingual immunotherapy is dose and duration dependent in a murine allergic rhinitis model Soichi Tofukuji, PhD, Kazufumi Katayama,

Genetic susceptibility to food allergy is linked to differential TH2-TH1 responses in C3H/HeJ and BALB/c mice Vivian Morafo, PhD*, Kamal Srivastava,

Mast cells regulate IFN-γ expression in the skin and circulating IgE levels in allergen- induced skin inflammation Harri Alenius, PhD, Dhafer Laouini,

TH1-promoting DNA immunization against allergens modulates the ratio of IgG1/IgG2a but does not affect the anaphylactic potential of IgG1 antibodies

Mohamed H. Shamji, PhD, FAAAI, Stephen R. Durham, MD, FRCP

A major allergen gene-fusion protein for potential usage in allergen-specific immunotherapy Fatimah Kussebi, MD, Fariba Karamloo, PhD, Claudio Rhyner,

Joaquim Mullol, MD, Joan C

TH1/TH2 cytokines and inflammatory cells in skin biopsy specimens from patients with chronic idiopathic urticaria: Comparison with the allergen-induced.

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Ligation of IgE receptors causes an anaphylactic response and neutrophil infiltration but does not induce eosinophilic inflammation in mice Masayuki.

Ellen Mueller Fox, PhD, Marina N

Toll-Like Receptor Activation during Cutaneous Allergen Sensitization Blocks Development of Asthma through IFN-Gamma-Dependent Mechanisms Rita Haapakoski,

The steroidogenic enzyme Cyp11a1 is essential for development of peanut-induced intestinal anaphylaxis Meiqin Wang, MD, PhD, Julita Ramirez, DVM, PhD,

Antigen coupled with Lewis-x trisaccharides elicits potent immune responses in mice Shih-Chang Hsu, BS, Tsung-Hsien Tsai, MD, Hirokazu Kawasaki, PhD,

DNA methylation of TH1/TH2 cytokine genes affects sensitization and progress of experimental asthma Stephanie Brand, PhD, Dörthe Andrea Kesper, PhD,

Inhibition of allergic airways inflammation and airway hyperresponsiveness in mice by dexamethasone: Role of eosinophils, IL-5, eotaxin, and IL-13 Seok-Yong.

Grass pollen immunotherapy: IL-10 induction and suppression of late responses precedes IgG4 inhibitory antibody activity James N. Francis, PhD, Louisa.

Suplatast tosilate inhibits goblet-cell metaplasia of airway epithelium in sensitized mice Jae Jeong Shim, MD, Karim Dabbagh, PhD, Kiyoshi Takeyama, MD,

Cytokine profile in minor salivary glands from patients with bronchial asthma Anne Tsicopoulos, MDa, b, Anne Janin, MDc, Hikmat Akoum, PhDa, Catherine.

Lymphocytes in Peyer patches regulate clinical tolerance in a murine model of food allergy Christophe P. Frossard, MS, Laurence Tropia, Conrad Hauser,

IL-4– and IL-5–positive T lymphocytes, eosinophils, and mast cells in allergen-induced late-phase cutaneous reactions in atopic subjects Luis T. Barata,

Allergen-specific T-cell tolerance induction with allergen-derived long synthetic peptides: Results of a phase I trial Jean-Marc Fellrath, MDa, Alexander.

Oral administration of a dominant T-cell determinant peptide inhibits allergen-specific TH1 and TH2 cell responses in Cry j 2–primed mice Kazuki Hirahara,

Ovalbumin-specific IgE modulates ovalbumin-specific T-cell response after repetitive oral antigen administration Nemuko Omata, MD, Yusei Ohshima, MD,

Reciprocal regulation of cultured human mast cell cytokine production by IL-4 and IFN-γ Hiroshi Tachimoto, MD, PhDa, Motohiro Ebisawa, MD, PhDa,b, Tomohide.

Local treatment with IL-12 is an effective inhibitor of airway hyperresponsiveness and lung eosinophilia after airway challenge in sensitized mice Jürgen.

Allergy multivaccines created by DNA shuffling of tree pollen allergens Michael Wallner, PhD, Angelika Stöcklinger, MSc, Theresa Thalhamer, MSc, Barbara.

Presentation transcript:

Suppression of antigen-specific T- and B-cell responses by intranasal or oral administration of recombinant Bet v 1, the major birch pollen allergen, in a murine model of type I allergy Ursula Wiedermann, MD, PhDa, Beatrice Jahn-Schmid, PhDa,b, Barbara Bohle, MSca, Andreas Repa a, Harald Renz, MDc, Dietrich Kraft, MDa, Christof Ebner, MDa Journal of Allergy and Clinical Immunology Volume 103, Issue 6, Pages 1202-1210 (June 1999) DOI: 10.1016/S0091-6749(99)70200-9 Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 1 Serum IgG1, IgG2a and IgE antibody levels against BP and recombinant Bet v 1 (rBet) . Open bars indicate mean optical density (OD) of 6 mice immunized intraperitoneally with rBet v 1/Al(OH)3 and subsequently aerosolized with BP extract (group 3). Hatched bars indicate mean OD of 6 mice orally treated with rBet v 1 before sensitization (group 2), and filled bars indicate mean OD of 6 mice intranasally administered rBet v 1 before sensitization (group 1). Error bars indicate SEMs. *P < .05, **P < .01 as determined by the Mann-Whitney U test. Journal of Allergy and Clinical Immunology 1999 103, 1202-1210DOI: (10.1016/S0091-6749(99)70200-9) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 2 Total serum IgG and IgE immunoglobulin levels. Open bars indicate mean optical density (OD) of 6 mice immunized intraperitoneally with rBet v 1/Al(OH)3 and subsequently aerosolized with BP extract (group 3). Hatched bars indicate mean OD of 6 mice orally treated with rBet v 1 before sensitization (group 2), and filled bars show mean OD of 6 mice intranasally administered rBet v 1 before sensitization (group 1). Error bars indicate SEMs. n.s., Nonsignificant as determined by the Mann-Whitney U test. Journal of Allergy and Clinical Immunology 1999 103, 1202-1210DOI: (10.1016/S0091-6749(99)70200-9) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 3 Representative skin sections of type I skin tests of mice sensitized with rBet v1/Al(OH)3 intraperitoneally (i.p.) before BP aerosol exposure (group 3), mice treated 3 times orally with 100 μg rBet v1 (group 2), and mice treated 3 times intranasally (i.n.) with 10 μg rBet v 1 before sensitization (group 1). Skin reactions to BP (2.5 μg/mL) and rBet v 1 (2.5 μg/mL), histamine-releasing compound 48/80 (20 μg/mL), and PBS were measured at the inside of the abdominal skin 20 minutes after intradermal injection. Journal of Allergy and Clinical Immunology 1999 103, 1202-1210DOI: (10.1016/S0091-6749(99)70200-9) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 4 Cytokine production in spleen cell cultures. IL-4 and IL-5 levels were measured after 24 hours, and IFN-γ and IL-10 levels were measured 48 hours after stimulation with BP. Open bars indicate mean values (pg/mL) of 6 mice immunized intraperitoneally with rBet v 1/Al(OH)3 and subsequently aerosolized with BP extract (group 3). Hatched bars indicate mean values (pg/mL) of 6 mice orally treated with rBet v 1 before sensitization (group 2), and black bars show mean values (pg/mL) of 6 mice intranasally administered rBet v1 before sensitization (group 1). Error bars indicate SEMs. *P < .05, **P < .001 as determined by the Mann-Whitney U test. Journal of Allergy and Clinical Immunology 1999 103, 1202-1210DOI: (10.1016/S0091-6749(99)70200-9) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 5 TGF-β mRNA expression in spleen cells of 5 sensitized (group 3) and 5 nasally tolerized mice (group 1). Bars indicate ratio of β-actin/TGF-β and are expressed as mean values of 4 individual PCR experiments. Error bars indicate SEMs. *P < .05 as determined by the Mann-Whitney U test. Journal of Allergy and Clinical Immunology 1999 103, 1202-1210DOI: (10.1016/S0091-6749(99)70200-9) Copyright © 1999 Mosby, Inc. Terms and Conditions

Fig. 6 Proposed mechanism of mucosal tolerance induction in a TH2 model. Mucosal (oral or nasal) administration of low doses of soluble antigen/allergen may lead to induction of a third subset of T-helper cells, termed TH3 (or Tr) cells, in the mucosa-associated lymphoid tissues. These regulatory T cells, which may also migrate to the systemic sites, produce inhibitory cytokines, such as TGF-β.9,23 The preferential production of this cytokine may then lead to an active suppression of antigen-specific TH2 and TH1 cells and consequently no antibody production to the respective antigen/allergen at the effector sites of the immune system. PP, Peyer’s patch; LP, lamina propria; APC, antigen-presenting cells. Journal of Allergy and Clinical Immunology 1999 103, 1202-1210DOI: (10.1016/S0091-6749(99)70200-9) Copyright © 1999 Mosby, Inc. Terms and Conditions