Basophils, high-affinity IgE receptors, and CCL2 in human anaphylaxis

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Presentation transcript:

Basophils, high-affinity IgE receptors, and CCL2 in human anaphylaxis Peter Korosec, PhD, Paul J. Turner, FRACP, PhD, Mira Silar, BSc, Peter Kopac, MD, Mitja Kosnik, MD, PhD, Bernhard F. Gibbs, PhD, Mohamed H. Shamji, PhD, Adnan Custovic, MD, PhD, Matija Rijavec, PhD Journal of Allergy and Clinical Immunology Volume 140, Issue 3, Pages 750-758.e15 (September 2017) DOI: 10.1016/j.jaci.2016.12.989 Copyright © 2017 The Authors Terms and Conditions

Fig 1 Basophil CD63 activation (A); absolute basophil counts (B); whole-blood FCER1A (C), CPA3 (D) and HDC (E) expression; serum tryptase levels (F); and PMN (G) and lymphocyte (H) absolute counts in ED patients during acute anaphylactic reactions to Hymenoptera venom and 7 and 30 days after the anaphylactic episode. Horizontal lines represent median values with interquartile ranges. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig 2 Comparison of basophil CD63 activation (A), absolute basophil counts (B), whole-blood FCER1A gene expression (C), CCL2 serum concentrations (D), and serum tryptase levels (E) between patients with acute anaphylactic reactions to Hymenoptera venom on ED presentation and patients with venom allergy or healthy control subjects. Horizontal lines represent median values with interquartile ranges. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig 3 Correlation between absolute basophil counts and whole-blood FCER1A (A), CPA3 (B), and HDC (C) gene expression and serum CCL2 concentrations (D) in patients with acute anaphylactic reactions presenting to the ED. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig 4 Serum CCL2 (A), CCL5 (B), CCL11 (C), IL-3 (D), and TSLP (E) levels in ED patients during acute anaphylactic reactions to Hymenoptera venom and 7 and 30 days after the anaphylactic episode. Horizontal lines represent median values with interquartile ranges. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig 5 Absolute basophil counts (A) and whole-blood FCER1A gene expression (B) in patients with peanut allergy undergoing DBPCFCs to peanut. Horizontal lines represent median values with interquartile ranges. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig 6 Serum CCL2 levels in allergic patients undergoing controlled DBPCFCs to peanut: A, absolute CCL2 levels; B, percentage change in CCL2 from baseline. Horizontal lines represent median values with interquartile ranges. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E1 Time between symptom onset and blood sample collection in ED patients with acute anaphylactic reactions. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E2 Correlation between basophil CD63 and CD203c activation in ED patients with acute anaphylactic reactions. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E3 Absolute basophil counts (A), whole-blood FCER1A gene expression (B), serum tryptase levels (C), and CCL2 serum concentrations (D) in ED patients divided according to the severity of acute allergic reactions (Mueller grade I-II vs grade III-IV) and then 7 and 30 days after the episode. The threshold for diagnostically positive tryptase measurement was set at 11.2 μg/L. Data are presented as a person-to-person scatter plot. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E4 Basophil CD63 activation (A), basophil absolute count (B), whole-blood FCER1A gene expression (C), and lymphocyte (D), and PMN (E) absolute counts in healthy control subjects 2.5 to 3, 5, and 24 hours after the single dose of oral methylprednisolone (64 mg). Horizontal lines represent median values with interquartile ranges. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E5 Serum concentrations of CCL2 (A), CCL5 (B), CCL11 (C), and IL-3 (D) in healthy control subjects 2.5 to 3, 5, and 24 hours after the single dose of oral methylprednisolone (64 mg). Horizontal lines represent median values with interquartile ranges. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E6 Basophil CD63 activation, absolute basophil counts, serum tryptase levels, PMN and lymphocyte absolute counts, and CCL2 serum concentrations in 2 ED patients (nos. 14 and 15, Table E1) sampled 1.5 hours, 24 hours, 7 days, and 1 month after the onset of symptoms. Both patients were treated with methylprednisolone. Data are presented as a before/after scatter plots. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E7 Basophil absolute counts, whole-blood FCER1A gene expression, serum tryptase levels, and CCL2 serum concentrations during acute anaphylactic reactions to Hymenoptera venom and 7 and 30 days after anaphylactic episodes in ED patients divided according to methylprednisolone treatment (patients 20 and 29 were not treated with methylprednisolone, Table E1). Data are presented as a person-to-person scatter plot. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions

Fig E8 ROC curve analysis of basophil CD63 activation, absolute basophil counts, whole-blood FCER1A gene expression, CCL2 concentrations, and serum tryptase levels between patients with acute anaphylactic reactions to insect venoms on ED presentation and patients with venom allergy or healthy control subjects. AUC, Area under the curve. Journal of Allergy and Clinical Immunology 2017 140, 750-758.e15DOI: (10.1016/j.jaci.2016.12.989) Copyright © 2017 The Authors Terms and Conditions