Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI Vanesa Y. Rawe, Heydy Uriondo Boudri, Cristian Alvarez Sedó,

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Healthy baby born after reduction of sperm DNA fragmentation using cell sorting before ICSI Vanesa Y. Rawe, Heydy Uriondo Boudri, Cristian Alvarez Sedó, Martina Carro, Sergio Papier, Florencia Nodar Reproductive BioMedicine Online Volume 20, Issue 3, Pages 320-323 (March 2010) DOI: 10.1016/j.rbmo.2009.12.004 Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 (A and B) Phase contrast and epifluorescent photographs of apoptotic spermatozoa during the diagnostic phase: (A) fragmented DNA, bright TUNEL-positive cells) and (B) cleaved caspase 3 (arrow heads: mid-piece). (C–E) TUNEL analysis during MACS and ICSI procedure in different fractions: (C) density gradient centrifugation/pre-MACS; (D) annexin V-negative; (E) annexin V-positive. (F) Good-quality transferred embryo 48h after MACS and ICSI. T=percentage of spermatozoa with fragmented DNA measured by TUNEL assay. Bar=10μm (A–E) and 20μm (F). Reproductive BioMedicine Online 2010 20, 320-323DOI: (10.1016/j.rbmo.2009.12.004) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions