A Consensus on Fungal Polymerase Chain Reaction Diagnosis?

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A Consensus on Fungal Polymerase Chain Reaction Diagnosis? P. Lewis White, Richard Barton, Malcolm Guiver, Christopher J. Linton, Steve Wilson, Melvyn Smith, Beatriz L. Gomez, Michael J. Carr, Patrick T. Kimmitt, Shila Seaton, Kumar Rajakumar, Tessa Holyoake, Chris C. Kibbler, Elizabeth Johnson, Richard P. Hobson, Brian Jones, Rosemary A. Barnes The Journal of Molecular Diagnostics Volume 8, Issue 3, Pages 376-384 (July 2006) DOI: 10.2353/jmoldx.2006.050120 Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Sensitivities of the Aspergillus PCR assays using different PCR platforms with different fungal loads and sample types. a: Roche LightCycler, b: Corbett Rotor–Gene, and c: Applied Biosystems TaqMan. The Journal of Molecular Diagnostics 2006 8, 376-384DOI: (10.2353/jmoldx.2006.050120) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Detection limits for the Aspergillus assays using the Roche LightCycler platform and Corbett Rotor–Gene with DNA extracted from EDTA whole blood spiked with Aspergillus conidia. a: 2Asp assay and b: 4Asp assay. The Journal of Molecular Diagnostics 2006 8, 376-384DOI: (10.2353/jmoldx.2006.050120) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Sequence similarity between a: 4Asp forward primer; b: 4Asp probe; c: A. fumigatus 18S rRNA gene partial sequence (accession no. AF548063, bp 1212 to 1367); d: Homo sapiens 18S rRNA gene partial sequence (accession no. X03205, bp 1346 to 1508); e–h: 4Asp LC products; and i: 4Asp reverse primer. The Journal of Molecular Diagnostics 2006 8, 376-384DOI: (10.2353/jmoldx.2006.050120) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Schematic proposing a structure for a pan-fungal PCR using the current methods as described and assessed. The Journal of Molecular Diagnostics 2006 8, 376-384DOI: (10.2353/jmoldx.2006.050120) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions