In vitro effect of unacylated ghrelin and obestatin on human luteal cell function  Federica Romani, M.D., Antonio Lanzone, M.D., Anna Tropea, M.D., Alessandra Familiari, M.D., Elisa Scarinci, M.D., Michela Sali, B.S., Giovanni Delogu, M.D., Stefania Catino, M.L.T., Rosanna Apa, M.D.  Fertility and Sterility  Volume 97, Issue 4, Pages 991-996 (April 2012) DOI: 10.1016/j.fertnstert.2012.01.096 Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Effect of unacylated ghrelin and obestatin on basal P release by human luteal cells. Luteal cells were cultured for 24 hours in medium alone (CTR), with hGC (100 ng/mL), with unacylated ghrelin (10−10 to 10−7 M), or with obestatin (10−10 to 10−7 M). Each value represents the mean ± SEM of 11 independent experiments, each done in triplicate. Results are expressed as percentage of CTR set equal to 100. ∗∗∗P<.001, ∗∗P<.01 vs. CTR values. Fertility and Sterility 2012 97, 991-996DOI: (10.1016/j.fertnstert.2012.01.096) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Effect of unacylated ghrelin and obestatin on basal PGE2 release by human luteal cells. Luteal cells were cultured for 24 hours in medium alone (CTR), with unacylated ghrelin (10−10 to 10−7 M), or with obestatin (10−10 to 10−7 M). Each value represents the mean ± SEM of nine independent experiments, each done in triplicate. Results are expressed as percentage of CTR set equal to 100. ∗∗P<.01 vs. CTR values. Fertility and Sterility 2012 97, 991-996DOI: (10.1016/j.fertnstert.2012.01.096) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Effect of unacylated ghrelin and obestatin on basal PGF2α release by human luteal cells. Luteal cells were cultured for 24 hours in medium alone (CTR), with unacylated ghrelin (10−10 to 10−7 M), or with obestatin (10−10 to 10−7 M). Each value represents the mean ± SEM of nine independent experiments, each done in triplicate. Results are expressed as percentage of CTR set equal to 100. ∗∗P<.01 vs. CTR values. Fertility and Sterility 2012 97, 991-996DOI: (10.1016/j.fertnstert.2012.01.096) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Effect of unacylated ghrelin and obestatin on basal VEGF release by human luteal cells. Luteal cells were cultured for 24 hours in medium alone (CTR), with CoCl2 (10 μM), with unacylated ghrelin (10−10 to 10−7 M), or with obestatin (10−10 to 10−7 M). Each value represents the mean ± SEM of 13 independent experiments, each done in triplicate. Results are expressed as percentage of CTR set equal to 100. ∗∗∗P<.001, ∗∗P<.01, ∗P<.05 vs. CTR values. Fertility and Sterility 2012 97, 991-996DOI: (10.1016/j.fertnstert.2012.01.096) Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions