Increasing the accuracy of peanut allergy diagnosis by using Ara h 2

Slides:

Advertisements

Similar presentations

A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay Jing Lin, PhD, Francesca M. Bruni,

Advertisements

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Characteristics of childhood peanut allergy in the Australian Capital Territory, 1995 to 2007 Raymond James Mullins, MBBS, PhD, FRACP, FRCPA, Keith B.G.

Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity Abena S. Amoah, MSc, Benedicta.

Does eczema in infancy cause hay fever, asthma, or both in childhood

Polymorphisms affecting vitamin D–binding protein modify the relationship between serum vitamin D (25[OH]D3) and food allergy Jennifer J. Koplin, PhD,

Skin prick test responses and allergen-specific IgE levels as predictors of peanut, egg, and sesame allergy in infants Rachel L. Peters, MPH, Katrina.

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

A randomized, double-blind, placebo-controlled pilot study of sublingual versus oral immunotherapy for the treatment of peanut allergy Satya D. Narisety,

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD

Ann-Marie M. Schoos, MD, PhD, Jacob D

Stephen J. Fowler, MD, Gaël Tavernier, PhD, Robert Niven, MD

Katrina J. Allen, MBBS, BMedSc, FRACP, PhD, Jennifer J

Diagnosis of red meat allergy with antigen-specific IgE tests in serum

Is 9 more than 2 also in allergic airway inflammation?

Breast-feeding and atopic disease: A cohort study from childhood to middle age Melanie Claire Matheson, BSc, MAppSc, PhD, Bircan Erbas, BSc, PhD, Aindralal.

Dean Tey, MBBS, FRACP, Katrina J

Blood DNA methylation biomarkers predict clinical reactivity in food-sensitized infants David Martino, PhD, Thanh Dang, PhD, Alexandra Sexton-Oates, BSc,

Loss of allergenic proteins during boiling explains tolerance to boiled peanut in peanut allergy Paul J. Turner, FRACP, PhD, Sam Mehr, FRACP, Rebekah.

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

Allergy testing in predicting outcome of open food challenge to peanut

Understanding the feasibility and implications of implementing early peanut introduction for prevention of peanut allergy Jennifer J. Koplin, PhD, Rachel.

Morten J. Christensen, MD, Per Stahl Skov, MD, DMSci, Lars K

Peter M. Wolfgram, MD, David B. Allen, MD

Etiology of asthma exacerbations

Skin prick test responses and allergen-specific IgE levels as predictors of peanut, egg, and sesame allergy in infants Rachel L. Peters, MPH, Katrina.

Can early introduction of egg prevent egg allergy in infants

Administration of a probiotic with peanut oral immunotherapy: A randomized trial Mimi L.K. Tang, PhD, Anne-Louise Ponsonby, PhD, Francesca Orsini, MSc,

Joseph A. Odhiambo, MMed, Hywel C. Williams, PhD, Tadd O

Polymorphisms affecting vitamin D–binding protein modify the relationship between serum vitamin D (25[OH]D3) and food allergy Jennifer J. Koplin, PhD,

Basophil activation test discriminates between allergy and tolerance in peanut- sensitized children Alexandra F. Santos, MD, MSc, Abdel Douiri, PhD, Natalia.

A randomized, double-blind, placebo-controlled pilot study of sublingual versus oral immunotherapy for the treatment of peanut allergy Satya D. Narisety,

Reply Journal of Allergy and Clinical Immunology

The prevalence of food allergy and other allergic diseases in early childhood in a population-based study: HealthNuts age 4-year follow-up Rachel L.

Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency.

Allergen reference doses for precautionary labeling (VITAL 2

Kerstin Bauermeister, MSc, Barbara K

Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management Scott H. Sicherer, MD, Hugh A. Sampson, MD Journal.

Biosimilars and drug development in allergic and immunologic diseases

Anaphylaxis fatalities and admissions in Australia

Effect of ingested H1 antihistamines on methacholine challenge

What is an “eosinophilic phenotype” of asthma?

Audrey DunnGalvin, PhD, L. M

Skin test evaluation of a novel peptide carrier–based vaccine, BM32, in grass pollen– allergic patients Verena Niederberger, MD, Katharina Marth, MD,

H. William Kelly, PharmD Journal of Allergy and Clinical Immunology

Autophagy: Nobel Prize 2016 and allergy and asthma research

Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment

Anaphylaxis to apple and orange seed

Advances in the approach to the patient with food allergy

The Utility of Peanut Components in the Diagnosis of IgE-Mediated Peanut Allergy Among Distinct Populations Jay A. Lieberman, MD, Susanne Glaumann, MD,

Paul V. Licciardi, PhD, Anne Balloch, MSc, Fiona M

Martin Brasholt, MD, Florent Baty, PhD, Hans Bisgaard, MD, DMSci

What can urban/rural differences in food allergy prevalence tell us about the drivers of food allergy? Katrina J. Allen, MBBS, BMedSc, FRACP, FAAAI,

Time trends in Australian hospital anaphylaxis admissions in to

Innate immune responses to infection

A bioinformatics approach to identify patients with symptomatic peanut allergy using peptide microarray immunoassay Jing Lin, PhD, Francesca M. Bruni,

Reply Journal of Allergy and Clinical Immunology

Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity Abena S. Amoah, MSc, Benedicta.

Macrolide antibiotics and asthma treatment

Statistical issues in clinical trials that involve the double-blind, placebo-controlled food challenge Vernon M. Chinchilli, PhD, Laura Fisher, MD, Timothy.

Asthma: The past, future, environment, and costs

Food allergy is associated with Staphylococcus aureus colonization in children with atopic dermatitis Andrea L. Jones, MD, Douglas Curran-Everett, PhD,

Functional assessment of pathogenic IgG subclasses in chronic autoimmune urticaria Sridharan Soundararajan, PhD, Yoko Kikuchi, DVM, Kusumam Joseph, PhD,

Probiotic supplementation for the first 6 months of life fails to reduce the risk of atopic dermatitis and increases the risk of allergen sensitization.

Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity Abena S. Amoah, MSc, Benedicta.

The use of serum-specific IgE measurements for the diagnosis of peanut, tree nut, and seed allergy Jennifer M. Maloney, MD, Magnus Rudengren, BSc, Staffan.

Does eczema in infancy cause hay fever, asthma, or both in childhood

Natural history of cow’s milk allergy

Traffic-related air pollution exposure is associated with allergic sensitization, asthma, and poor lung function in middle age Gayan Bowatte, BSc, Caroline.

Presentation transcript:

Increasing the accuracy of peanut allergy diagnosis by using Ara h 2 Thanh D. Dang, BBiomedSc (Hons), Mimi Tang, MBBS, PhD, FRACP, FRCPA, FAAAAI, Sharon Choo, MBBS, FRACP, FRCPA, Paul V. Licciardi, PhD, Jennifer J. Koplin, PhD, Pamela E. Martin, BBiomedSc (Hons), Tina Tan, BSc, Lyle C. Gurrin, PhD, Anne-Louise Ponsonby, BMedSc, MBBS, PhD, FAFPHM, FRACP, Dean Tey, MBBS, FRACP, Marnie Robinson, MBBS, FRACP, Shyamali C. Dharmage, MBBS, MD, PhD, Katrina J. Allen, BMedSc, MBBS, FRACP, PhD Journal of Allergy and Clinical Immunology Volume 129, Issue 4, Pages 1056-1063 (April 2012) DOI: 10.1016/j.jaci.2012.01.056 Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Selection of subjects for Ara h 2 testing. Journal of Allergy and Clinical Immunology 2012 129, 1056-1063DOI: (10.1016/j.jaci.2012.01.056) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 ROC curves showing true-positive rates (sensitivity) plotted against the false-positive rate (specificity) for different cutoff points of the quantified components of Ara h 2 (red squares) and whole peanut extract (blue circles). The points highlighted are the current conventional SPT (3 and 8 mm) and whole peanut sIgE (0.35 and 15 kUA/L) thresholds. The points highlighted for Ara h 2 indicate putative levels for determining sensitization (0.1 kUA/L) and peanut allergy (1.0 kUA/L). The area under the curve is 0.95, 0.94, and 0.89 for Ara h 2 sIgE, peanut SPT, and peanut sIgE, respectively. Journal of Allergy and Clinical Immunology 2012 129, 1056-1063DOI: (10.1016/j.jaci.2012.01.056) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Ara h 1 or Ara h 3 concentrations of 0.35 kUA/L or greater in patients with peanut allergy with levels of less than 0.35 kUA/L for Ara h 2 (n = 19). The whole peanut concentrations are shown at the end of the 4 columns for each patient. Journal of Allergy and Clinical Immunology 2012 129, 1056-1063DOI: (10.1016/j.jaci.2012.01.056) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Various alternatives of diagnosing peanut allergy with peanut sIgE measurements (A), peanut SPTs (B), or Ara h 2 sIgE measurements (C) followed by an OFC. Patients from this study were examined by using the current conventional cutoffs for peanut sIgE measurements and SPTs to determine the stringency of each test. PA, Patients with peanut allergy; PT, peanut-tolerant subjects. Journal of Allergy and Clinical Immunology 2012 129, 1056-1063DOI: (10.1016/j.jaci.2012.01.056) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Various clinical scenarios for diagnosing peanut allergy by using a 3-step model in a community setting (A) or an allergy clinic setting (B). Peanut sIgE measurements or peanut SPTs were assessed as the first-line test followed by Ara h 2 sIgE measurements to help improve the diagnosis of peanut allergy when either peanut sIgE or peanut SPT test results fall into the respective cutoffs of either 0.35 to 15 kUA/L or 3 to 8 mm, respectively. PA, Patients with peanut allergy who fall into the designated ranges; PT, peanut-tolerant subjects who fall into the designated ranges. Journal of Allergy and Clinical Immunology 2012 129, 1056-1063DOI: (10.1016/j.jaci.2012.01.056) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions