Identification of Functional Patterns of Androgenetic Alopecia Using Transcriptome Profiling in Distinct Locations of Hair Follicles Yong Miao, Qian.

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Identification of Functional Patterns of Androgenetic Alopecia Using Transcriptome Profiling in Distinct Locations of Hair Follicles Yong Miao, Qian Qu, MD, Wei Jiang, Xiao-Min Liu, Pan-Li Shi, Zhe-Xiang Fan, Li-Juan Du, Gao-Feng Wang, Xiao-Nan Liu, Zhen-He Guo, Yang Liu, Fang Liu, Ya-Rui Liu, Zhi-Qi Hu Journal of Investigative Dermatology Volume 138, Issue 4, Pages 972-975 (April 2018) DOI: 10.1016/j.jid.2017.10.027 Copyright © 2017 The Authors Terms and Conditions

Figure 1 Dissection diagram of hair follicle and basic analysis of differentially expressed genes. Representative images of hair follicle (a) harvested by follicular unit extraction and (b) after dissection. (c) Schematic illustration of the hair follicle indicates the two main parts in this transcriptome analysis (blue arrows indicate part I [bugle-containing portion] and part II [DP-containing portion]. A large grid represents 1 mm. (d) Heat map of inflammation-related genes with significant changes in six groups. Genes expressed at low levels are in grey; those expressed at high levels are in red. (e) Abnormal expression of target genes mediating hair follicle stem cell pools in the ABB and ANB groups. Dash indicates FDR > 0.1. DP, dermal papilla; FDR, false discovery rate. Journal of Investigative Dermatology 2018 138, 972-975DOI: (10.1016/j.jid.2017.10.027) Copyright © 2017 The Authors Terms and Conditions

Figure 2 Validation of some selected genes by quantitative real-time reverse transcriptase–PCR, immunofluorescence, Western blot, and network analysis. (a) Validation of selected genes (inflammation/stress/fibrosis related: JUN, FOSB, EGR1, KRT16, CDSN, COL1A1, COL3A1; psoriasis related: SERPINA3, S100A7, S100A3, PSORS1C2; hair disorders related: TCHH) by quantitative real-time reverse transcriptase–PCR. (b) Western blot proved increased level of psoriasin (S100a7, highly expressed in psoriasis), FOSB, and EGR1 in AGA groups. Immunostaining images showed up-regulation of (c) FOSB (red) and (d) psoriasin (green) in DP and bulge-containing area in AGA. Scale bars = 100 μm. (e) Average gray value represented signal intensity of FOSB and psoriasin. (f) Network analysis of 14 psoriasis-related genes and five AGA-related genes. Psoriasis-related genes are marked by green circles, and other AGA-related genes are marked by yellow circles. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001, ∗∗∗∗P < .0001. ABD: AGA, balding area, DP-containing part; ABB: AGA, balding area, bugle-containing part; AGA: androgenetic alopecia; AND: AGA, nonbalding area, DP-containing part; ANB: AGA, nonbalding area, bugle-containing part; CND: control, nonbalding area, DP-containing part; CNB: control, nonbalding area, bugle-containing part; DP: dermal papilla. Journal of Investigative Dermatology 2018 138, 972-975DOI: (10.1016/j.jid.2017.10.027) Copyright © 2017 The Authors Terms and Conditions