by T. G. Willis, I. R. Zalcberg, L. J. A. Coignet, I. Wlodarska, M

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Molecular Cloning of Translocation t(1;14)(q21;q32) Defines a Novel Gene (BCL9) at Chromosome 1q21 by T.G. Willis, I.R. Zalcberg, L.J.A. Coignet, I. Wlodarska, M. Stul, D.M. Jadayel, C. Bastard, J.G. Treleaven, D. Catovsky, M.L.M. Silva, and M.J.S. Dyer Blood Volume 91(6):1873-1881 March 15, 1998 ©1998 by American Society of Hematology

T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology

T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology

FISH results for BCL9 probes. FISH results for BCL9 probes. (A) Localization of PAC containing BCL9 sequences to chromosome 1q21 on normal metaphases. (B) Rearrangement of CEPH YAC 882B3 in case no. 2 with t(1;22)(q21;q11), showing hybridization signals at the breakpoints of both der(1) and der(22) chromosomes. (C) Metaphase of case no. 7 showing YAC 882B3 hybridization signals on normal 1 and der(19) chromosomes. Amplification of hybridization signal on the add(1) is shown by a solid arrow. Note the extra unidentified material between centromere and YAC signal. T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology

Northern analysis of BCL9 expression in a variety of normal human tissues. Northern analysis of BCL9 expression in a variety of normal human tissues. A multiple tissue Northern blot was hybridized with the BCL9 probe X665. Reprobing with a GAPDH probe showed equal RNA loading in all samples (data not shown). T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology

T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology

T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology

Northern blot analysis of 10 μg total RNA from various cell lines of B-cell lineage. Northern blot analysis of 10 μg total RNA from various cell lines of B-cell lineage. ASLCL, an EBV-transformed normal B-lymphoblastoid cell line acted as negative control. Both a truncated 6.3-kb and the 4.2-kb BCL9 transcript were overexpressed in the CEMO-1 cell line but not in any other B-cell lines with 1q21 abnormalities (Table 1). T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology

Southern blot analysis of DNA from case no Southern blot analysis of DNA from case no. 6 and normal control using probe X665. Southern blot analysis of DNA from case no. 6 and normal control using probe X665. Two rearranged signals were seen inBgl II and Xba I digests, indicating that the breakpoint fell within the region of this probe in the 3′ UTR ofBCL9. T.G. Willis et al. Blood 1998;91:1873-1881 ©1998 by American Society of Hematology