Molecular Cell Biology 5068 Bob Mercer, rmercer@wustl.edu 362-6924; 991-1243

Goals For MCB 5068 1. Obtain a solid foundation of knowledge in cell biology 2. Obtain a working knowledge of available techniques. 3. Be able to critically read and evaluate the scientific literature. 4. Be able to define and investigate a biological problem.

TA’s: Victoria Ismail rogersv@wustl.edu Aisha Lee aishalee@wustl.edu Brittany Townley townleyba@wustl.edu

Lectures: Tuesday and Thursday, 8:30-10:00 AM, Erlanger Auditorium Molecular Cell Biology 5068 http://mcb5068.wustl.edu Lectures: Tuesday and Thursday, 8:30-10:00 AM, Erlanger Auditorium 9/18 & 10/11 in Holden Auditorium Discussion Section: Wednesday, 3-4 PM Papers assigned One-page summary/critique expected

Molecular Cell Biology 5068 To Do: Visit website: www.mcb5068.wustl.edu Sign up for course Check out Self Assessment homework Visit Discussion Sections: Read “Official” Instructions

Molecular Cell Biology 5068

Discussion Section Techniques Protein purification using GST Fusion CRISPER/Cas 9 gene editing SDS-PAGE/Immunoblotting/Immunoprecipitation Immunofluorescence Sanger Sequencing Real time PCR Proliferation Assay/BrdU In vivo intracellular pH determination/pHluorin Size exclusion chromatography

Grading: 3 in class closed book exams accounting for 75% of grade 25% of final grade based on performance in Discussion Section Percentage final grades last 2 years: 36% A 28% A- 21% B+ 14% B 0.5% B- 0.5% C+