Marguerite Stas, Hugo Degreef Journal of Investigative Dermatology

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Analysis of N- and K-Ras Mutations in the Distinctive Tumor Progression Phases of Melanoma Marguerite Stas, Hugo Degreef Journal of Investigative Dermatology Volume 117, Issue 6, Pages 1483-1489 (December 2001) DOI: 10.1046/j.0022-202x.2001.01601.x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Microdissection of the distinctive growth phases of MM. Frozen section of a SSMM Clark level IV showing the various growth phases, i.e. ‘‘pure’’ RGP (1), the ‘‘invasive’’ RGP (2) and the VGP (3) before (A) and after (B) microdissection Journal of Investigative Dermatology 2001 117, 1483-1489DOI: (10.1046/j.0022-202x.2001.01601.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Sensitivity of the DGGE mutation detection assay. (A) To demonstrate the sensitivity of the DGGE, a mutant sample (lane 2) (codon 18 mutation) was mixed with a wild type sample (lane 3) to generate heteroduplexes (lane 1). Mutations were clearly resolved by showing a band pattern deviant from that of the wild type. (B) DNA from MOLT-4 cell line (heterozygous for mutation in N-RAS codon 12 position 1) was serially diluted with normal human spleen and revealed a detection sensitivity of 2.5% mutant DNA in wild type DNA. Lane 1 to 9 represents the dilution of the MOLT-4 cell line (100%, 80%, 60%, 40%, 20%, 10%, 5%, 2.5%, and 0% in lane 9). Lane 10 shows a wild type pattern of a wild type sample. Journal of Investigative Dermatology 2001 117, 1483-1489DOI: (10.1046/j.0022-202x.2001.01601.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 N-ras gene point mutations observed on a DGGE gel. A 20-50% DGGE gel showing the migration pattern for 9 samples in N-ras exon 2 (lanes 1 to 9) and 2 samples with aberrations in N-ras exon 1 (lane 10 and 11). Case 1 to 4 showing a transversion from CAA (glutamine) to CTA (leucine) in codon 61 of N-ras exon 2. Lanes 5 and 8 exhibit a change from CAA (glutamine) to AAA (lysine) in codon 61 of N-ras exon 2. Lanes 6, 7 and 9 contain wild type samples. Lanes 10 and 11 show mutations in codon 12 of N-ras exon 1 (GGT (glycine) to GAT (asparagine) transition). Journal of Investigative Dermatology 2001 117, 1483-1489DOI: (10.1046/j.0022-202x.2001.01601.x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions