Volume 19, Issue 3, Pages 205-214 (June 2012) HIF-3α mRNA expression changes in different tissues and their role in adaptation to intermittent hypoxia and physical exercise  T. Drevytska, B. Gavenauskas, S. Drozdovska, V. Nosar, V. Dosenko, I. Mankovska  Pathophysiology  Volume 19, Issue 3, Pages 205-214 (June 2012) DOI: 10.1016/j.pathophys.2012.06.002 Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Relative level of all HIF subunits mRNA expression in different tissues at acute hypoxia (12% O2 during 2h). A, HIF-1α; B, HIF-1β; C, HIF-2α; D, HIF-3α. White-filled bar, control level; dark-filled bar, acute hypoxia. *P<0.05 compared to control. Pathophysiology 2012 19, 205-214DOI: (10.1016/j.pathophys.2012.06.002) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Relative level of mRNA HIF-3α expression in different tissues in control (□), acute hypoxia (), intermittent hypoxic training () and acute hypoxia after intermittent hypoxic training (■). *P<0.05 compared to control; #P<0.05 compared to acute hypoxia without IHT. Pathophysiology 2012 19, 205-214DOI: (10.1016/j.pathophys.2012.06.002) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Relative level of mRNA HIF-1α (A), HIF-2α (B) and HIF-3α (C) expression in different tissues at control (□), endurance training (), endurance training with IHT (■). *P<0.05 compared to control; #P<0.05 compared to endurance training without IHT. Pathophysiology 2012 19, 205-214DOI: (10.1016/j.pathophys.2012.06.002) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 Influence of endurance training and intermittent hypoxic training on functional parameters of endurance. (A) the time of swimming to exhaustion (min). Meaning of the groups is indicated in Section 2. *Significant difference (P<0.05) between control group (group 1) and group with swimming training (group 2) or with swimming training+IHT (group 3). #Significant difference (P<0.05) between group 2 and group 3. (B) maximal oxygen consumption (ml/min×100g). *Significantly different (P<0.05) when compared with the control group. Pathophysiology 2012 19, 205-214DOI: (10.1016/j.pathophys.2012.06.002) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

Fig. 5 Influence of endurance training and intermittent hypoxic training on oxygen tension in m. gastrocnemius. (□) Before standard testing intensive physical workload; (■) after standard testing intensive physical loading. *Significantly different (P<0.05) when compared with initial level in control group; #Significantly different (P<0.05) between before and after testing intensive physical workload. Pathophysiology 2012 19, 205-214DOI: (10.1016/j.pathophys.2012.06.002) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

Fig. 6 Influence of antiHIF-3α siRNA injection on gene expression: A, HIF-3α; B, IGF-1; C, GLUT-4; D, PDK-1. *Significantly different (P<0.05) compared to scrRNA injection. Pathophysiology 2012 19, 205-214DOI: (10.1016/j.pathophys.2012.06.002) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions

Fig. 7 Endurance (min) in rats with endurance training and siRNA. Control (□); endurance training+scrRNA, 3 weeks (); endurance training+siRNA, 3 weeks (), endurance training+scrRNA, 5weeks (), endurance training+siRNA, 5weeks (■). *Significant difference (P<0.05) between control group (group 1) and group with endurance training with scrRNA (group 2) or endurance training with siRNA (group 3); #Significantly different (P<0.05) between group 2 and group 3. Pathophysiology 2012 19, 205-214DOI: (10.1016/j.pathophys.2012.06.002) Copyright © 2012 Elsevier Ireland Ltd Terms and Conditions