Deficient expression of JMJD1A histone demethylase in patients with round spermatid maturation arrest  Zahra Eelaminejad, Raha Favaedi, Niloofar Sodeifi, Mohammad Ali Sadighi Gilani, Maryam Shahhoseini  Reproductive BioMedicine Online  Volume 34, Issue 1, Pages 82-89 (January 2017) DOI: 10.1016/j.rbmo.2016.09.005 Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 (A) Relative mRNA expression of the JMJD1A gene normalized to β-actin in samples with OA (n = 10), SMA (n = 11) and SCOS (n = 10). (B) Total chromatin incorporation of JMJD1A protein normalized to core histone H2A content in OA (n = 5), SMA (n = 7) and SCOS (n = 6) groups. OA = obstructive azoospermia; SCOS = Sertoli cell only syndrome; SMA = round spermatid maturation arrest. Data are shown as mean ± SEM and were analysed using a Kruskal−Wallis test with Dunn's post-hoc test. Asterisks indicate significant differences compared with control (OA), **P < 0.005. Reproductive BioMedicine Online 2017 34, 82-89DOI: (10.1016/j.rbmo.2016.09.005) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Immunohistochemistry of JMJD1A in human testicular sections. (A, B) Testicular sections of samples with OA (obstructive azoospermia) stained with anti-JMJD1A antibody. (C) Testicular section of a sample with OA without anti-JMJD1A antibody. (D, E) Testicular sections of samples with SMA (round spermatid maturation arrest) stained with anti-JMJD1A antibody. (F) Testicular section of a sample with SMA without anti-JMJD1A antibody. (G, H) Testicular sections of samples with SCOS (Sertoli cell only syndrome) stained with anti-JMJD1A antibody. (I) Testicular section of a sample with SCOS without anti-JMJD1A antibody. CJF = cytoplasmic JMJD1A foci; ES = elongating spermatids; MS = mature spermatids; pSC = pachytene spermatocytes; RS = round spermatids; SC = Sertoli cells; SG = spermatogonia. Scale bar = 20 µm. Reproductive BioMedicine Online 2017 34, 82-89DOI: (10.1016/j.rbmo.2016.09.005) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 (A) Chromatin immunoprecipitation of JMJD1A protein on the promoter regions of TNP1, TNP2, PRM1 and PRM2 genes in patients with OA (n = 12), SMA (n = 12) and SCOS (n = 10). (B) Relative mRNA expression normalized to β-actin of TNP1, TNP2, PRM1 and PRM2 genes in OA (n = 10), SMA (n = 11) and SCOS (n = 10) groups. OA = obstructive azoospermia; SMA = round spermatid maturation arrest; SCOS = Sertoli cell only syndrome. Statistical significance for multiple comparisons was determined by a Kruskal−Wallis test with Dunn's post-hoc test (mean ± SEM). Asterisks indicate significant differences compared with control (OA), **P < 0.005, *P < 0.05. Hash shows significant difference between SMA and SCOS groups, #P < 0.05. Reproductive BioMedicine Online 2017 34, 82-89DOI: (10.1016/j.rbmo.2016.09.005) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions