Molecular characterization and susceptibility of methicillin-resistant and methicillin- susceptible Staphylococcus aureus isolates from hospitals and the.

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Molecular characterization and susceptibility of methicillin-resistant and methicillin- susceptible Staphylococcus aureus isolates from hospitals and the community in Vladivostok, Russia T. Baranovich, H. Zaraket, I.I. Shabana, V. Nevzorova, V. Turcutyuicov, H. Suzuki Clinical Microbiology and Infection Volume 16, Issue 6, Pages 575-582 (June 2010) DOI: 10.1111/j.1469-0691.2009.02891.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 SmaI macrorestriction patterns of 63 Staphylococcus aureus isolates from four hospital laboratories in Vladivostok, Russia and their genetic types. The pulsed-field gel electrophoresis (PFGE) types (PFTs) were defined by ≥80% similarity (UPMAG, Dice). Isolates with PFGE patterns with similarity greater than 95% were considered to belong to the same PFT. PFGE patterns of one representative isolate from each PFT are shown. The number of isolates in each PFT cluster is shown on the left side of the dendogram. The molecular characteristics of each bacterial strain are listed in Tables 2 and 3. *The novel spa type. The novel sequence type (ST). MRSA, methicillin-resistant Staphylococcus aureus; MSSA, methicillin-susceptible S. aureus. Clinical Microbiology and Infection 2010 16, 575-582DOI: (10.1111/j.1469-0691.2009.02891.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Schematic figure of the proposed genetic relationship among spa types associated with Panton--Valentine leukocidin (PVL)--positive ST121 methicillin-susceptible Staphylococcus aureus (MSSA) isolates. These spa types shared a common genetic background (similar order of the repeats and 100% homology among shared repeats), but differed in the deletion and/or insertion of some repeats or point mutations within the repeat. spa type 287 was unique for PVL--positive ST121 MSSA isolates (n = 4) that were associated with fatal community-acquired MSSA pneumonia in this study. Asterisks indicate the terminal M repeat. Clinical Microbiology and Infection 2010 16, 575-582DOI: (10.1111/j.1469-0691.2009.02891.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions