Boulder Electron Microscopy Services

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Boulder Electron Microscopy Services Serving University of Colorado labs, other universities and corporate researchers for 30+ years Provides full range of transmission EM services Conventional TEM, electron tomography and cryo-electron microscopy Training: specimen preparation, microscope use, 3D software. this will be illustrated by the published work displayed as the background of the slides I’ll show today Kang et al., Traffic 12:313-329, 2011

Boulder Electron Microscopy Services Ueda et al., PNAS 108:17099-104, 2011.

Boulder Electron Microscopy Services: Conventional EM Specimen preparation: HPF and freeze substitution Thin section electron microscopy Negative stain Protein localization with immuno-gold Erickson et al., PlosPathog 8:e1002630, 2012

Boulder Electron Microscopy Services: 3D cellular tomography Automated image acquisition using IVEMs Software training for computing and modeling 3D reconstructions O’Toole and Dutcher, Cytoskeleton, 71:108-118, 2013

Boulder Electron Microscopy Services: Cryo-electron microscopy Specimen preparation: Manual and Vitrobot plunge freezers. Cryo-electron microscopy/tomography 3D volume averaging software Cope et al., J Struct Biol. 170:257-265, 2010

Boulder Electron Microscopy Services https://mcdb.colorado.edu/facilities/ems Who we are and where we live: Tom Giddings, Director Mary Morphew Courtney Ozzello Eileen O’Toole Department of MCD Biology, Porter Biosciences (303) 492-8402 BoulderEM@colorado.edu West et al., J. Cell Biol. 193:333-46, 2011.