Serum Androgens and Genetic Linkage Analysis in Early Onset Androgenetic Alopecia  J. Pardinas, D. Whiting, H.I. Katz, V. Price, A. Zlotogorski, J. Roberts, B.C. Clark  Journal of Investigative Dermatology  Volume 113, Issue 2, Pages 277-279 (August 1999) DOI: 10.1046/j.1523-1747.1999.00659.x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Serum androgen levels in the three families with EE-AGA. ID#, subject's identification number; Age, the age at the time of blood draw and photo documentation; Type, the Norwood or Ludwig patterns (females) at the time of blood draw; age-o/e, age of onset/age of establishment of a particular AGA pattern. T (Testosterone) and D (Dihydrotestosterone) values are presented as pg per ml. The serum androgens were assayed by published methods (Abraham 1981;Walker et al. 1996). Black squares represent EE-AGA male individuals. Journal of Investigative Dermatology 1999 113, 277-279DOI: (10.1046/j.1523-1747.1999.00659.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Serum androgens and genetic linkage analysis. (A) DHT/T ratios in three families. The analysis of variance (anova) was conducted using StatView software, Version 4 (Abacus Concepts, Berkeley, CA) for combined sex including all individuals (with and without EE-AGA). p values of significance in the DHT/T ratio between the three families reported in the text were based on Bonferroni/Dunn test. (B) DHT/T ratio between males with and without EE-AGA. p value of significance reported in the text was calculated by anova using SAS/JMP software, version 3.1.5 (SAS Institute, Carey, NC). Ten severely affected males with EE-AGA whose serum samples were available and used for comparative androgen analysis are indicated by an asterisk below their black square symbols in Figure 1 One individual (F-11 #23), who had residual hair on the frontal scalp, was included as unaffected for comparative androgen analysis along with 19 males without EE-AGA (white squares). Error bars indicate the standard error. (C) Multipoint LOD and NPL statistic from the GENEHUNTER analysis of chromosome-2 markers. Multipoint LOD score (dominant affected only model) and NPL values are shown by solid and knotted lines, respectively, with the marker names (from p to q arm) on the x-axis. An aliquot of PCR genotyping reactions with fluorescence tagged primers were analyzed on an Applied Biosystems machine with Genescan 672 and Genotyper 1.1 software. Linkage analysis was performed using GENEHUNTER computer package (Kruglyak et al. 1996) on a SUN Ultra-1 workstation, and MLINK program of the LINKAGE package, version 5.1 (Terwilliger & Ott 1994). Journal of Investigative Dermatology 1999 113, 277-279DOI: (10.1046/j.1523-1747.1999.00659.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions