Breadth of binding and HAI activity of serum Ab from i. n. + i. p

Slides:



Advertisements
Similar presentations
Figure 1. Antibody responses against homologous 2013 H7 virus in H7N9-infected patients. A, Serum samples from H7N9-infected patients were collected at.
Advertisements

Serum IgG (A–D) and IgA (E–H) titer from BALB/c mice (n = 5/group) at day 0 or following COBRA P1 virus i.n., i.p., or i.m. prime at day 21 or following.
CD154 Expression Is Associated with Neutralizing Antibody Titer Levels Postinfluenza Vaccination in Stem Cell Transplant Patients and Healthy Adults 
Isabella Maiellaro, Martin J. Lohse, Robert J. Kittel, Davide Calebiro 
Blocking antibodies induced by immunization with a hypoallergenic parvalbumin mutant reduce allergic symptoms in a mouse model of fish allergy  Raphaela.
by Clemens B. Caspar, Shoshana Levy, and Ronald Levy
Volume 16, Issue 5, Pages (August 2016)
(A) NK cell cytotoxicity assays against K562, H9, and Raji target cell lines in the presence (black) and absence (white) of an anti-NKp30 antibody, clone.
Volume 138, Issue 4, Pages (August 2009)
Early CMV Viremia Is Associated with Impaired Viral Control following Nonmyeloablative Hematopoietic Cell Transplantation with a Total Lymphoid Irradiation.
Immunogenicity and Safety of Seasonal Influenza Vaccination in Patients with Classic Kaposi's Sarcoma  Monica Cappelletti, Adriano Taddeo, Elena Colombo,
M. Koopmans, E. de Bruin, G. -J. Godeke, I. Friesema, R
(A) Weight loss curves following intranasal infection of different mouse groups with COBRA, seasonal, or pandemic influenza viruses. (A) Weight loss curves.
Longitudinal analysis of cross-group HA stem–binding lineages.
Volume 20, Issue 7, Pages (August 2017)
HA-specific Abs in serum 14 d after a single vaccination with the various targeted DNA vaccines. HA-specific Abs in serum 14 d after a single vaccination.
A single DNA vaccination of C57BL/6 mice induced Ab responses of IgG1 and/or IgG2c subclass. A single DNA vaccination of C57BL/6 mice induced Ab responses.
Sialylated IgG1 gp120-specific Abs are increased in neutralizers.
Replacing mouse BAFF with human BAFF does not improve B-cell maturation in hematopoietic humanized mice by Julie Lang, Bicheng Zhang, Margot Kelly, Jacob.
Volume 15, Issue 4, Pages (April 2014)
Volume 3, Issue 4, Pages (April 2013)
Serum IgG subclass representation from BALB/c mice (n = 5/group) following COBRA P1 virus i.n., i.p., or i.m. prime followed by i.p. or i.m. boost. Serum.
Colonization with N. musculi results in Ab production.
Protection from influenza virus challenge.
B-1a and B-1b Cells Exhibit Distinct Developmental Requirements and Have Unique Functional Roles in Innate and Adaptive Immunity to S. pneumoniae  Karen.
Volume 43, Issue 3, Pages (September 2015)
Notes Over 8.3 Simplifying Natural Base Expressions
Volume 17, Issue 4, Pages (April 2010)
Volume 43, Issue 6, Pages (December 2015)
STAT3 regulates IgE class switching in a Th2-type response.
Modified mRNA Vaccines Protect against Zika Virus Infection
Volume 16, Issue 6, Pages (August 2016)
Adapted viruses replicate in mismatched miR-122-expressing cells.
MMV has fungicidal activity at lower cell concentrations.
Volume 23, Issue 3, Pages (April 2018)
Volume 16, Issue 5, Pages (August 2016)
Volume 25, Issue 5, Pages (October 2018)
Volume 19, Issue 3, Pages (March 2011)
Volume 25, Issue 12, Pages e3 (December 2018)
Favorable safety profile of LV‐mediated intracerebral gene therapy
IL-27 contributes to IR expression by lung T cells during toxoplasmosis. IL-27 contributes to IR expression by lung T cells during toxoplasmosis. (A) Il27p28-GFP.
Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp for each of 13 serotypes. Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp.
Volume 24, Issue 7, Pages (August 2018)
Patient and control ONS cells had very large differences in gene expression. Patient and control ONS cells had very large differences in gene expression.
NKTfh cell–driven class switch against polysaccharide Ag is IL-21-influenced. NKTfh cell–driven class switch against polysaccharide Ag is IL-21-influenced.
Severity and Phenotype of Bullous Pemphigoid Relate to Autoantibody Profile Against the NH2- and COOH-Terminal Regions of the BP180 Ectodomain  Silke.
Superspacers shared between viral strains.
Sialylated IgG1 gp120-specific Abs are increased in neutralizers.
Ligands eluted from hpMR1+EC and hpMR1+MS contain both shared and unique ions. Ligands eluted from hpMR1+EC and hpMR1+MS contain both shared and unique.
Schematic representation of the immunization regimens used in this study. Schematic representation of the immunization regimens used in this study. BALB/c.
CD27+ expression correlates with robust Ag-specific responses to M
IL-27 induces expression of multiple IR by CD8+ T cells.
Anti-DNA Abs were not affected by the deletion of GCs in pristane-induced lupus model. Anti-DNA Abs were not affected by the deletion of GCs in pristane-induced.
Increased anti-DNA Abs in 2KO-Bcl6TC mice.
Volume 15, Issue 4, Pages (April 2014)
Responses measured by BOLD fMRI in areas V1, V2, V3, and MT+ as two subjects (S2, gray; S3, black) switched between the speed and the contrast discrimination.
Antibody titers against surface antigens of B
Atsushi Yamanaka, Eiji Konishi
Functional characterization of multidonor class antibodies.
Scattergrams showing IgG- and IgA-specific OD490 values measured with the Campylobacter-specific P18/P39 ELISA in sera from healthy controls and from GBS.
Fig. 6 Multivalent DNA vaccine and protein antigen delivery by T4-AAV nanoparticles. Multivalent DNA vaccine and protein antigen delivery by T4-AAV nanoparticles.
Fig. 2 NDR2-deficient mice are more vulnerable to RNA viral infection.
Antibody titers against surface antigens of M
Changes in autoantibody titers and GADA IgG1–4 subclass distribution.
Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus tissues after stimulation in vivo with poly(I:C). Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus.
Fig. 1 Induction of IgM and IgG following controlled human P
Fig. 2 Induction and waning kinetics of merozoite-specific IgM during naturally acquired P. falciparum malaria. Induction and waning kinetics of merozoite-specific.
Lkt antibody titers of vaccinated and control groups of BHS
Fig. 3 MCA-mediated neutrophil recruitment accelerates bacterial clearance in the skin. MCA-mediated neutrophil recruitment accelerates bacterial clearance.
Active Alp1 protease induces both AHR and eosinophilic inflammation.
Presentation transcript:

Breadth of binding and HAI activity of serum Ab from i. n. + i. p Breadth of binding and HAI activity of serum Ab from i.n. + i.p. vaccinated groups (COBRA: P1 [n = 15], X3 [n = 15], X6 [n = 15]; seasonal: Chile/83 [n = 10], Sing/86 [n = 5], NC/99 [n = 10], and Brisb/07 [n = 10], and pandemic H1N1: CA/09 [n = 6]), using COBRA, seasonal, or pandemic H1N1 viral strains. Breadth of binding and HAI activity of serum Ab from i.n. + i.p. vaccinated groups (COBRA: P1 [n = 15], X3 [n = 15], X6 [n = 15]; seasonal: Chile/83 [n = 10], Sing/86 [n = 5], NC/99 [n = 10], and Brisb/07 [n = 10], and pandemic H1N1: CA/09 [n = 6]), using COBRA, seasonal, or pandemic H1N1 viral strains. White dots indicate the IgG binding titer against COBRA (P1, X3, and X6) and multiple H1N1 rHA expressed as area under curve (AUC) of the obtained OD414 values from 3-fold serial diluted sera on the different rHA proteins. The y-axes on the right of each graph are expressed as antilog of Log10. The continuous black lines indicate the AUC values obtained on cH6/1 rHA protein and approximately representing the contribution of stem-binding Abs. Black bars indicate the HAI titers (left y-axis) against COBRA (P1, X3, and X6) and multiple H1N1 viruses (x-axis). Titers below the limit of detection were assigned a value of 5 for the purpose of calculating the geometric mean titer and are not visible on this scale. The y-axes along the left of each graph are expressed as Log2. Values are reported as absolute mean values plus SEM obtained in a single experiment and using sera from different mouse groups (A–H). Data are representative of three independent experiments evaluating the breadth of binding and HAI activity of sera from the same mouse groups. For each independent experiment, mouse sera were assayed in duplicate. Black dotted lines delimiting the gray area indicate the 1:40–1:80 HAI titer range. Giuseppe A. Sautto et al. ImmunoHorizons 2018;2:226-237 Copyright © 2018 The Authors