Molecular Diagnosis of Cystoisosporiasis Using Extended-Range PCR Screening  Sean C. Murphy, Daniel R. Hoogestraat, Dhruba J. SenGupta, Jennifer Prentice, Andrea Chakrapani, Brad T. Cookson  The Journal of Molecular Diagnostics  Volume 13, Issue 3, Pages 359-362 (May 2011) DOI: 10.1016/j.jmoldx.2011.01.007 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Molecular diagnosis of cystoisosporiasis using molecular methods and retrospective confirmation by histologic examination. A: PCR-based detection of C. belli using primers directed against the 28S rRNA gene and ITS regions 1 and 2. PCR reactions were performed on extracted DNA, and PCR products were separated by electrophoresis using a 1% agarose gel and stained. Reaction products from primer sets are shown with the patient template (Pt), positive control (+), and negative control (−), as shown. A 100-bp DNA ladder is also shown. B: C. belli parasites demonstrated in the ileal mucosa in a biopsy specimen from the described patient. Multinucleated asexual schizonts (arrow), sexual macrogametocyte (wide arrowhead), and microgametocytes (narrow arrowheads) reside within enterocytes. The gastrointestinal epithelium demonstrated a normal-appearing epithelial layer with intact lamina propria lacking significant inflammation. Hematoxylin and eosin stain; original magnification ×40. The Journal of Molecular Diagnostics 2011 13, 359-362DOI: (10.1016/j.jmoldx.2011.01.007) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions