Volume 10, Issue 11, Pages (November 2017)

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Volume 10, Issue 11, Pages 1465-1468 (November 2017) PacBio Sequencing Reveals Transposable Elements as a Key Contributor to Genomic Plasticity and Virulence Variation in Magnaporthe oryzae  Jiandong Bao, Meilian Chen, Zhenhui Zhong, Wei Tang, Lianyu Lin, Xingtan Zhang, Haolang Jiang, Deyu Zhang, Chenyong Miao, Haibao Tang, Jisen Zhang, Guodong Lu, Ray Ming, Justice Norvienyeku, Baohua Wang, Zonghua Wang  Molecular Plant  Volume 10, Issue 11, Pages 1465-1468 (November 2017) DOI: 10.1016/j.molp.2017.08.008 Copyright © 2017 The Authors Terms and Conditions

Figure 1 Whole-Genome Characterization of TE, Gene, and Chromosomal Translocation in Magnaporthe oryzae. (A) Chromosomes/contigs. Long contigs more than 50 kb were sorted and orientated according to chromosomes of 70-15. (B) Secreted proteins (SPs) frequency calculated in 10 kb non-overlapping sliding windows. (C) Gene distribution. Presence/absence of variation of genes was analyzed with an additional 60 isolates. Blue lines indicate core genes present in all of the additional 60 isolates and green lines indicate lineage-specific (LS) genes, which have presence/absence of polymorphism in the 60 isolates. (D) Distribution of transposable elements (TEs). (E) Inter-chromosomal translocation. Different colored lines from different chromosome linked to same contig indicate translocation events. (F) LS regions but not isolate-specific regions contain more SP genes. Genes absent in any other isolate are defined as a “unique gene” marked in red. (G) Proportion of TE-associated genes that encode SPs is higher than non-SPs. Genes with a 1 kb flanking region inserted by TE are defined as TE-associated genes. (H) Large-scale inter-chromosomal translocation events detected by whole-genome alignments using Murasaki. The chromosome name and length of 70-15 (denoted as 7015) are shown on the x axis; contig name (number) and length of FJ81278 (FJ, green) and Guy11 (G, red) are shown on the y axis. Gray lines on the x axis represent the chromosome end of 70-15, while green and red lines on the y axis represent FJ81278 and Guy11 contigs, respectively. (I) TE mediated the Chr1/Chr6 inter-chromosome rearrangement in FJ81278, validated by long-read mapping with Sniffles. This event was also observed in the third contig of FJ81278 (A; marked with an asterisk) and shown as a green broken anti-diagonal line in (H). Molecular Plant 2017 10, 1465-1468DOI: (10.1016/j.molp.2017.08.008) Copyright © 2017 The Authors Terms and Conditions